Volume 2, Issue 12 1870111
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Dynamic Biomaterials: Cyclic Stiffness Modulation of Cell-Laden Protein–Polymer Hydrogels in Response to User-Specified Stimuli Including Light (Adv. Biosys. 12/2018)

Luman Liu

Luman Liu

Department of Chemical Engineering, University of Washington, 3781 Okanogan Lane NE, Seattle, WA, 98195 USA

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Jared A. Shadish

Jared A. Shadish

Department of Chemical Engineering, University of Washington, 3781 Okanogan Lane NE, Seattle, WA, 98195 USA

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Christopher K. Arakawa

Christopher K. Arakawa

Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA, 98105 USA

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Kevin Shi

Kevin Shi

Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA, 98105 USA

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Jennifer Davis

Jennifer Davis

Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA, 98105 USA

Department of Pathology, University of Washington, 1959 NE Pacific St., Seattle, WA, 98195 USA

Institute for Stem Cell & Regenerative Medicine, University of Washington, 850 Republican St., Seattle, WA, 98109 USA

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Cole A. DeForest

Cole A. DeForest

Department of Chemical Engineering, University of Washington, 3781 Okanogan Lane NE, Seattle, WA, 98195 USA

Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA, 98105 USA

Institute for Stem Cell & Regenerative Medicine, University of Washington, 850 Republican St., Seattle, WA, 98109 USA

Molecular Engineering & Sciences Institute, University of Washington, 3946 W Stevens Way NE, Seattle, WA, 98195 USA

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First published: 07 December 2018
Citations: 3

Graphical Abstract

In article number 1800240, Cole A. DeForest and co-workers demonstrate that hydrogel biomaterials crosslinked with stimuli-responsive fusion proteins can undergo fully reversible stiffening upon exposure to user-specified environmental cues. Gels built upon optogenetic proteins LOV2 and Jα soften near-instantaneously under blue light, enabling spatiotemporal control of hydrogel compliance in the presence of living cells.

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