Stereoselective hydrolysis of optically active isomeric isopulegyl acetates by rhodotorula mucilaginosa and bacillus sp.
Abstract
A method for isolation of optically pure l-isopulegol from a mixture of its optically active isomers using the microorganisms Rhodotorula mucilaginosa and Bacillus sp. is described.
Microorganisms hydrolyzed l-isopulegyl acetate (26–40%) and in a small degree d-isopulegol acetate whereas the d-neoisopulegol acetate remained non-hydrolyzed.
The optical purity of the chromatographie pure l-isopulegol was 97.6%.