Volume 5, Issue 3 pp. 263-269
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Stereoselective hydrolysis of optically active isomeric isopulegyl acetates by rhodotorula mucilaginosa and bacillus sp.

A. Monkiewicz

A. Monkiewicz

Institute of General Food Chemistry, Technical University of Lódź Stefanowskiego 4/10, 90–924 Lódź

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J. Góra

J. Góra

Institute of General Food Chemistry, Technical University of Lódź Stefanowskiego 4/10, 90–924 Lódź

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Abstract

A method for isolation of optically pure l-isopulegol from a mixture of its optically active isomers using the microorganisms Rhodotorula mucilaginosa and Bacillus sp. is described.

Microorganisms hydrolyzed l-isopulegyl acetate (26–40%) and in a small degree d-isopulegol acetate whereas the d-neoisopulegol acetate remained non-hydrolyzed.

The optical purity of the chromatographie pure l-isopulegol was 97.6%.

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