Chapter 5
Bioscaffolding: A New Innovative Fabrication Process
Rania Abdelgaber,
David Kilian,
Hendrik Fiehn,
Rania Abdelgaber
Technische Universität Dresden, Centre for Translational Bone, Joint and Soft Tissue Research, Medical Faculty Carl Gustav Carus, Fetscherstrasse 74, 01307, Dresden, Germany
Search for more papers by this authorDavid Kilian
Technische Universität Dresden, Centre for Translational Bone, Joint and Soft Tissue Research, Medical Faculty Carl Gustav Carus, Fetscherstrasse 74, 01307, Dresden, Germany
Search for more papers by this authorRania Abdelgaber,
David Kilian,
Hendrik Fiehn,
Rania Abdelgaber
Technische Universität Dresden, Centre for Translational Bone, Joint and Soft Tissue Research, Medical Faculty Carl Gustav Carus, Fetscherstrasse 74, 01307, Dresden, Germany
Search for more papers by this authorDavid Kilian
Technische Universität Dresden, Centre for Translational Bone, Joint and Soft Tissue Research, Medical Faculty Carl Gustav Carus, Fetscherstrasse 74, 01307, Dresden, Germany
Search for more papers by this authorBook Editor(s):Mohammed Maniruzzaman,
Mohammed Maniruzzaman
School of Life Sciences, University of Sussex, Brighton, UK, BN1 9QG
Search for more papers by this authorFirst published: 14 December 2018
Summary
Biofabrication refers to the automated generation of constructs that can closely mimic the complex heterogenous nature and geometry of tissues and organs more than the current regenerative therapies do. This chapter presents an overview about the possibilities of mainly extrusion-based manufacturing technologies to create scaffolds for three-dimensional (3D) tissue models and techniques to approach a simulated natively heterogenous tissue structure. Scaffolds are designed with the aim of mimicking the in vivo microenvironment of the cells where cell-cell and cell-extracellular matrix (ECM) interactions occur, triggering cellular behavior and tissue function. Macroarchitecture describes the three-dimensional geometry of the scaffolds that has the ability to promote cell proliferation and differentiation, designed to mimic the native ECM of the cells in an optimal way. Currently, 3D bioscaffolds are mostly printed to study the cell growth or for performing the basic material and viability tests. Multimaterial printing describes the capability to print 3D objects from materials with very different properties.
References
-
De Deyne, P.G. and Kladakis, S.M. (2005). Bioscaffolds in tissue engineering: a rationale for use in the reconstruction of musculoskeletal soft tissues. Clinics in Podiatric Medicine and Surgery
22 (4): 521–532. https://doi.org/10.1016/j.cpm.2005.07.006.
10.1016/j.cpm.2005.07.006 Google Scholar
- Malda, J., Visser, J., Melchels, F.P. et al. (2013). 25th anniversary article: engineering hydrogels for biofabrication. Advanced Materials 25 (36): 5011–5028. https://doi.org/10.1002/adma.201302042.
- Langer, R. and Vacanti, J.P. (1993). Tissue engineering. Science 260 (May): 920–926. https://doi.org/10.1126/science.8493529.
- Do, A., Khorsand, B., Geary, S.M., and Salem, A.K. (2015). 3D printing of scaffolds for tissue regeneration applications. Advanced Healthcare Materials 4 (12): 1742–1762. https://doi.org/10.1002/adhm.201500168.3D.
- Groll, J., Boland, T., Blunk, T. et al. (2016). Biofabrication: reappraising the definition of an evolving field. Biofabrication 8 (1): https://doi.org/10.1088/1758-5090/8/1/013001.
-
Gurtner, G.C., Callaghan, M.J., and Longaker, M.T. (2007). Progress and potential for regenerative medicine. Annual Review of Medicine
58 (1): 299–312. https://doi.org/10.1146/annurev.med.58.082405.095329.
10.1146/annurev.med.58.082405.095329 Google Scholar
- Chen, F.M. and Liu, X. (2016). Advancing biomaterials of human origin for tissue engineering. Progress in Polymer Science 53: 86–168. https://doi.org/10.1016/j.progpolymsci.2015.02.004.
- Singh, D., Singh, D., and Han, S.S. (2016). 3D printing of scaffold for cells delivery: advances in skin tissue engineering. Polymer 8 (1): 1–17. https://doi.org/10.3390/polym8010019.
- Kang, H.W., Lee, S.J., Ko, I.K. et al. (2016). A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nature Biotechnology 34 (3): 312–319. https://doi.org/10.1038/nbt.3413.
- Yang, S., Leong, K.-F., Du, Z., and Chua, C.-K. (2001). The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Engineering 7 (6): 679–689. https://doi.org/10.1089/107632701753337645.
- Chia, H.N. and Wu, B.M. (2015). Recent advances in 3D printing of biomaterials. Journal of Biological Engineering 9 (1): 1–14. https://doi.org/10.1186/s13036-015-0001-4.
- Sydney Gladman, A., Matsumoto, E.A., Nuzzo, R.G. et al. (2016). Biomimetic 4D printing. Nature Materials 15 (4): 413–418. https://doi.org/10.1038/nmat4544.
- Loh, Q.L. and Choong, C. (2013). Three-dimensional scaffolds for tissue engineering applications: role of porosity and pore size. Tissue Engineering Part B: Reviews 19 (6): 485–502. https://doi.org/10.1089/ten.teb.2012.0437.
- Leong, K.F., Cheah, C.M., and Chua, C.K. (2003). Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs. Biomaterials 24 (13): 2363–2378. https://doi.org/10.1016/S0142-9612(03)00030-9.
- Laronda, M.M., Rutz, A.L., Xiao, S. et al. (2017). A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice. Nature Communications 8 (May): 1–10. https://doi.org/10.1038/ncomms15261.
- Chan, B.P. and Leong, K.W. (2008). Scaffolding in tissue engineering: general approaches and tissue-specific considerations. European Spine Journal 17: suppl. 4). https://doi.org/10.1007/s00586-008-0745-3.
- O'Brien, F.J. (2011). Biomaterials & scaffolds for tissue engineering. Materials Today 14 (3): 88–95. https://doi.org/10.1016/S1369-7021(11)70058-X.
- Gariboldi, M.I. and Best, S.M. (2015). Effect of ceramic scaffold architectural parameters on biological response. Frontiers in Bioengineering and Biotechnology 3 (October): 1–11. https://doi.org/10.3389/fbioe.2015.00151.
- Murphy, S.V. and Atala, A. (2014). 3D bioprinting of tissues and organs. Nature Biotechnology 32 (8): 773–785. https://doi.org/10.1038/nbt.2958.
- Chung, J.H.Y., Naficy, S., Yue, Z. et al. (2013). Bio-ink properties and printability for extrusion printing living cells. Biomaterials Science 1 (7): 763. https://doi.org/10.1039/c3bm00012e.
- He, Y., Yang, F., Zhao, H. et al. (2016). Research on the printability of hydrogels in 3D bioprinting. Scientific Reports 6: 1–13. https://doi.org/10.1038/srep29977.
- Nicodemus, G.D. and Bryant, S.J. (2008). Cell encapsulation in biodegradable hydrogels for tissue engineering applications. Tissue Engineering Part B: Reviews 14 (2): 149–165. https://doi.org/10.1089/ten.teb.2007.0332.
- Baumann, B., Jungst, T., Stichler, S. et al. (2017). Control of nanoparticle release kinetics from 3D printed hydrogel scaffolds. Angewandte Chemie – International Edition 56 (16): 4623–4628. https://doi.org/10.1002/anie.201700153.
- Axpe, E. and Oyen, M.L. (2016). Applications of alginate-based bioinks in 3D bioprinting. International Journal of Molecular Sciences 17.
- Niklason, L.E. and Langer, R.S. (1997). Advances in tissue engineering of blood vessels and other tissues. Transplant Immunology 5 (4): 303–306. https://doi.org/10.1016/S0966-3274(97)80013-5.
- Lode, A., Meyer, M., Brüggemeier, S. et al. (2016). Additive manufacturing of collagen scaffolds by three-dimensional plotting of highly viscous dispersions. Biofabrication 8 (1): https://doi.org/10.1088/1758-5090/8/1/015015.
-
Ahlfeld, T., Akkineni, A.R., Förster, Y. et al. (2017). Design and fabrication of complex scaffolds for bone defect healing: combined 3D plotting of a calcium phosphate cement and a growth factor-loaded hydrogel. Annals of Biomedical Engineering
45 (1): 224–236. https://doi.org/10.1007/s10439-016-1685-4.
10.1007/s10439-016-1685-4 Google Scholar
- Tu, X., Wang, L., Wei, J. et al. (2016). 3D printed PEGDA microstructures for gelatin scaffold integration and neuron differentiation. Microelectronic Engineering 158: 30–34. https://doi.org/10.1016/j.mee.2016.03.007.
- Shipley, R.J., Jones, G.W., Dyson, R.J. et al. (2009). Design criteria for a printed tissue engineering construct: a mathematical homogenization approach. Journal of Theoretical Biology 259 (3): 489–502. https://doi.org/10.1016/j.jtbi.2009.03.037.
- Luo, Y., Lode, A., Akkineni, A.R., and Gelinsky, M. (2015). Concentrated gelatin/alginate composites for fabrication of predesigned scaffolds with a favorable cell response by 3D plotting. RSC Advances 5 (54): 43480–43488. https://doi.org/10.1039/C5RA04308E.
- Moroni, L., Boland, T., Burdick, J.A. et al. (2017). Biofabrication: a guide to technology and terminology. Trends in Biotechnology https://doi.org/10.1016/j.tibtech.2017.10.015.
- Kilian, D., Ahlfeld, T., Akkineni, A.R. et al. (2017). Three-dimensional bioprinting of volumetric tissues and organs. MRS Bulletin 42 (8): 585–592.
- Markstedt, K., Mantas, A., Tournier, I. et al. (2015). 3D bioprinting human chondrocytes with nanocellulose-alginate bioink for cartilage tissue engineering applications. Biomacromolecules 16 (5): 1489–1496.
- Schütz, K., Placht, A.M., Paul, B. et al. (2017). Three-dimensional plotting of a cell-laden alginate/methylcellulose blend: towards biofabrication of tissue engineering constructs with clinically relevant dimensions. Journal of Tissue Engineering and Regenerative Medicine 11 (5): 1574–1587. https://doi.org/10.1002/term.2058.
- Müller, M., Becher, J., Schnabelrauch, M., and Zenobi-Wong, M. (2015). Nanostructured pluronic hydrogels as bioinks for 3D bioprinting. Biofabrication 7 (3): 35006. https://doi.org/10.1088/1758-5090/7/3/035006.
- Jia, J., Richards, D.J., Pollard, S. et al. (2014). Engineering alginate as bioink for bioprinting. Acta Biomaterialia 10 (10): 4323–4331. https://doi.org/10.1016/j.actbio.2014.06.034.
- Duarte Campos, D.F., Blaeser, A., Weber, M. et al. (2013). Three-dimensional printing of stem cell-laden hydrogels submerged in a hydrophobic high-density fluid. Biofabrication 5 (1): https://doi.org/10.1088/1758-5082/5/1/015003.
- Wu, Z., Su, X., Xu, Y. et al. (2016). Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation. Scientific Reports 6 (1): 24474. https://doi.org/10.1038/srep24474.
- Köpf, M., Campos, D.F.D., Blaeser, A. et al. (2016). A tailored three-dimensionally printable agarose – collagen blend allows encapsulation, spreading, and attachment of human umbilical artery smooth muscle cells. Biofabrication 8 (2): 1–15.
- Yeo, M.G. and Kim, G.H. (2017). A cell-printing approach for obtaining hASC-laden scaffolds by using a collagen/polyphenol bioink. Biofabrication 9: https://doi.org/https://doi.org/10.1088/1758-5090/aa6997.
- Kim, B.S., Kim, H., Gao, G. et al. (2017). Decellularized extracellular matrix: a step towards the next generation source for bioink manufacturing. Biofabrication 9 (3): https://doi.org/10.1088/1758-5090/aa7e98.
- Ahlfeld, T., Cidonio, G., Kilian, D. et al. (2017). Development of a clay based bioink for 3D cell printing for skeletal application. Biofabrication 9 (3): https://doi.org/10.1088/1758-5090/aa7e96.
-
Stichler, S., Jungst, T., Schamel, M. et al. (2017). Thiol–ene clickable poly(glycidol) hydrogels for biofabrication. Annals of Biomedical Engineering
45 (1): 273–285. https://doi.org/10.1007/s10439-016-1633-3.
10.1007/s10439-016-1633-3 Google Scholar
- Kesti, M., Eberhardt, C., Pagliccia, G. et al. (2015). Bioprinting complex cartilaginous structures with clinically compliant biomaterials. Advanced Functional Materials 25 (48): 7406–7417. https://doi.org/10.1002/adfm.201503423.
- Freeman, F.E. and Kelly, D.J. (2017). Tuning alginate bioink stiffness and composition for controlled growth factor delivery and to spatially direct MSC Fate within bioprinted tissues. Scientific Reports 7 (1): 1–12. https://doi.org/10.1038/s41598-017-17286-1.
- Di Luca, A., Klein-Gunnewiek, M., Vancso, J.G. et al. (2017). Covalent binding of bone morphogenetic protein-2 and transforming growth factor-β3 to 3D plotted scaffolds for osteochondral tissue regeneration. Biotechnology Journal 12 (12): 1–9. https://doi.org/10.1002/biot.201700072.
- Schuurman, W., Khristov, V., Pot, M.W. et al. (2011). Bioprinting of hybrid tissue constructs with tailorable mechanical properties. Biofabrication 3 (2): https://doi.org/10.1088/1758-5082/3/2/021001.
- Zehnder, T., Freund, T., Demir, M. et al. (2016). Fabrication of cell-loaded two-phase 3D constructs for tissue engineering. Materials 9 (11): https://doi.org/10.3390/ma9110887.
- Kolesky, D.B., Homan, K.A., Skylar-Scott, M.A., and Lewis, J.A. (2016). Three-dimensional bioprinting of thick vascularized tissues. Proceedings of the National Academy of Sciences 113 (12): 3179–3184. https://doi.org/10.1073/pnas.1521342113.
- Detsch, R., Sarker, B., Zehnder, T. et al. (2014). Additive manufacturing of cell-loaded alginate enriched with alkaline phosphatase for bone tissue engineering application. BioNanoMaterials 15 (3–4): 79–87. https://doi.org/10.1515/bnm-2014-0007.
- Criscenti, G., Longoni, A., Di Luca, A. et al. (2016). Triphasic scaffolds for the regeneration of the bone ligament interface. Biofabrication 8 (1): 015009.
- Brown, T.D., Dalton, P.D., and Hutmacher, D.W. (2011). Direct writing by way of melt electrospinning. Advanced Materials 23 (47): 5651–5657. https://doi.org/10.1002/adma.201103482.
- Perez, R.A. and Kim, H.W. (2015). Core–shell designed scaffolds for drug delivery and tissue engineering. Acta Biomaterialia 21: 2–19. https://doi.org/10.1016/j.actbio.2015.03.013.
- Akkineni, A.R., Ahlfeld, T., Lode, A., and Gelinsky, M. (2016). A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs. Biofabrication 8 (4): 1–15. https://doi.org/10.1088/1758-5090/8/4/045001.
- Luo, Y., Lode, A., Wu, C. et al. (2015). Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering. ACS Applied Materials and Interfaces 7 (12): 6541–6549. https://doi.org/10.1021/am508469h.
- Mistry, P., Aied, A., Alexander, M. et al. (2017). Bioprinting using mechanically robust core–shell cell-laden hydrogel strands. Macromolecular Bioscience 17 (6): 1–8. https://doi.org/10.1002/mabi.201600472.
- Onoe, H., Okitsu, T., Itou, A. et al. (2013). Metre-long cell-laden microfibres exhibit tissue morphologies and functions. Nature Materials 12 (6): 584–590. https://doi.org/10.1038/nmat3606.
-
Ahn, S., Lee, H., and Kim, G. (2013). Functional cell-laden alginate scaffolds consisting of core/shell struts for tissue regeneration. Carbohydrate Polymers
98 (1): 936–942. https://doi.org/10.1016/j.carbpol.2013.07.008.
10.1016/j.carbpol.2013.07.008 Google Scholar
- Tabriz, G., Ayala, H., and Nicholas, R. (2015). Three-dimensional bioprinting of complex cell laden alginate hydrogel structures. Biofabrication https://doi.org/10.1088/1758-5090/7/4/045012.
- Roth, E.A., Xu, T., Das, M. et al. (2004). Inkjet printing for high-throughput cell patterning. Biomaterials 25 (17): 3707–3715. https://doi.org/10.1016/j.biomaterials.2003.10.052.
- Okamoto, T., Suzuki, T., and Yamamoto, N. (2000). Microarray fabrication with covalent attachment of DNA using Bubble Jet technology. Nature Biotechnology 18 (4): 438–441. https://doi.org/10.1038/74507.
- Xu, T., Jin, J., Gregory, C. et al. (2005). Inkjet printing of viable mammalian cells. Biomaterials 26 (1): 93–99. https://doi.org/10.1016/j.biomaterials.2004.04.011.
-
Wilson, W.C. and Boland, T. (2003). Cell and organ printing 1: protein and cell printers. The Anatomical Record
272A (2): 491–496. https://doi.org/10.1002/ar.a.10057.
10.1002/ar.a.10057 Google Scholar
- Cui, X. and Boland, T. (2009). Human microvasculature fabrication using thermal inkjet printing technology. Biomaterials 30 (31): 6221–6227. https://doi.org/10.1016/j.biomaterials.2009.07.056.
- Saunders, R.E. and Derby, B. (2014). Inkjet printing biomaterials for tissue engineering: bioprinting. International Materials Reviews 59 (8): 430–448. https://doi.org/10.1179/1743280414Y.0000000040.
- Hoch, E., Hirth, T., Tovar, G.E.M., and Borchers, K. (2013). Chemical tailoring of gelatin to adjust its chemical and physical properties for functional bioprinting. Journal of Materials Chemistry B 1 (41): 5675. https://doi.org/10.1039/c3tb20745e.
- Xu, C., Chai, W., Huang, Y., and Markwald, R.R. (2012). Scaffold-free inkjet printing of three-dimensional zigzag cellular tubes. Biotechnology and Bioengineering 109 (12): 3152–3160. https://doi.org/10.1002/bit.24591.
- Arai, K., Iwanaga, S., Toda, H. et al. (2011). Three-dimensional inkjet biofabrication based on designed images. Biofabrication 3 (3): https://doi.org/10.1088/1758-5082/3/3/034113.
- Yamaguchi, S., Ueno, A., Akiyama, Y., and Morishima, K. (2012). Cell patterning through inkjet printing of one cell per droplet. Biofabrication 4 (4): https://doi.org/10.1088/1758-5082/4/4/045005.
- Christensen, K., Xu, C., Chai, W. et al. (2015). Freeform inkjet printing of cellular structures with bifurcations. Biotechnology and Bioengineering 112 (5): 1047–1055. https://doi.org/10.1002/bit.25501.
- Giron, S., Lode, A., and Gelinsky, M. (2016). In situ functionalization of scaffolds during extrusion-based 3D plotting using a piezoelectric nanoliter pipette. Journal of 3D Printing in Medicine 1 (1).
- Detsch, R., Blob, S., Zehnder, T., and Boccaccini, A.R. (2016). Evaluation of cell inkjet printing technique for biofabrication. BioNanoMaterials 17 (3–4): 185–191. https://doi.org/10.1515/bnm-2016-0007.
- Hutmacher, D.W., Sittinger, M., and Risbud, M.V. (2004). Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems. Trends in Biotechnology 22 (7): 354–362. https://doi.org/10.1016/j.tibtech.2004.05.005.
- Heller, M., Bauer, H.-K., Goetze, E. et al. (2016). Materials and scaffolds in medical 3D printing and bioprinting in the context of bone regeneration. International Journal of Computerized Dentistry 19 (4): 301–321. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/28008428.
- Hinton, T.J., Jallerat, Q., Palchesko, R.N. et al. (2015). Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels. Science Advances 1 (9): e1500758. https://doi.org/10.1126/sciadv.1500758.
- Krujatz, F., Lode, A., Seidel, J. et al. (2017). Additive Biotech—chances, challenges, and recent applications of additive manufacturing technologies in biotechnology. New Biotechnology 39 (September): 222–231.
- Lode, A., Krujatz, F., Brüggemeier, S. et al. (2015). Green bioprinting: fabrication of photosynthetic algae-laden hydrogel scaffolds for biotechnological and medical applications. Engineering in Life Sciences 15 (2): 177–183. https://doi.org/10.1002/elsc.201400205.
- Seidel, J., Ahlfeld, T., Adolph, M. et al. (2017). Green bioprinting: extrusion-based fabrication of plant cell-laden biopolymer hydrogel scaffolds. Biofabrication 9 (4): https://doi.org/10.1088/1758-5090/aa8854.
- Hong, N., Yang, G.-H., Lee, J., and Kim, G. (2017). 3D bioprinting and its in vivo applications. Journal of Biomedical Materials Research Part B: Applied Biomaterials 444–459. https://doi.org/10.1002/jbm.b.33826.
- Shim, J.H., Won, J.Y., Park, J.H. et al. (2017). Effects of 3D-printed polycaprolactone/β-tricalcium phosphate membranes on guided bone regeneration. International Journal of Molecular Sciences 18 (5): https://doi.org/10.3390/ijms18050899.
- Cubo, N., Garcia, M., Cañizo, J.F. et al. (2016). 3D bioprinting of functional human skin: production and in vivo analysis. Biofabrication 9 (1): 15006. https://doi.org/10.1088/1758-5090/9/1/015006.
- Chhaya, M.P., Melchels, F.P.W., Holzapfel, B.M. et al. (2015). Sustained regeneration of high-volume adipose tissue for breast reconstruction using computer aided design and biomanufacturing. Biomaterials 52 (1): 551–560. https://doi.org/10.1016/j.biomaterials.2015.01.025.
- Jia, W., Gungor-ozkerim, P.S., Zhang, Y.S. et al. (2016). Direct 3D bioprinting of perfusable vascular constructs using a blend bioink. Biomaterials 106: 58–68.
-
Costa, P.F., Vaquette, C., Baldwin, J. et al. (2014). Biofabrication of customized bone grafts by combination of additive manufacturing and bioreactor knowhow. Biofabrication
6 (3): 35006. https://doi.org/10.1088/1758-5082/6/3/035006.
10.1088/1758-5082/6/3/035006 Google Scholar
- Schneeberger, K., Spee, B., Costa, P. et al. (2017). Converging biofabrication and organoid technologies: the next frontier in hepatic and intestinal tissue engineering? Biofabrication 9 (1): https://doi.org/10.1088/1758-5090/aa6121.
- Edmondson, R., Broglie, J.J., Adcock, A.F., and Yang, L. (2014). Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors. Assay and Drug Development Technologies 12 (4): 207–218. https://doi.org/10.1089/adt.2014.573.
- Gao, B., Yang, Q., Zhao, X. et al. (2016). 4D bioprinting for biomedical applications. Trends in Biotechnology 34 (9): 746–756. https://doi.org/10.1016/j.tibtech.2016.03.004.
- Stoychev, G., Puretskiy, N., and Ionov, L. (2011). Self-folding all-polymer thermoresponsive microcapsules. Soft Matter 7 (7): 3277. https://doi.org/10.1039/c1sm05109a.
- Hendrikson, W.J., Rouwkema, J., Clementi, F. et al. (2017). Towards 4D printed scaffolds for tissue engineering: exploiting 3D shape memory polymers to deliver time-controlled stimulus on cultured cells. Biofabrication 9 (3): https://doi.org/10.1088/1758-5090/aa8114.
