Chapter 12
Small-Volume Hyphenated NMR Techniques
Andrew Webb,
Andrew Webb
Leiden University Medical Center, C.J. Gorter Center for High Field MRI, Department of Radiology, C3Q, Albinusdreef 2, 2333 AZ Leiden, The Netherlands
Search for more papers by this authorAndrew Webb,
Andrew Webb
Leiden University Medical Center, C.J. Gorter Center for High Field MRI, Department of Radiology, C3Q, Albinusdreef 2, 2333 AZ Leiden, The Netherlands
Search for more papers by this authorJens Anders,
Jan G. Korvink,
Jens Anders
University of Stuttgart, Institute of Smart Sensors, Pfaffenwaldring 47, Stuttgart, 70569 Germany
Search for more papers by this authorJan G. Korvink
Karlsruhe Institute of Technology, Institute of Microstructure Technology, Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen, 76344 Germany
Search for more papers by this authorBook Author(s):Jens Anders,
Jan G. Korvink,
Jens Anders
University of Stuttgart, Institute of Smart Sensors, Pfaffenwaldring 47, Stuttgart, 70569 Germany
Search for more papers by this authorJan G. Korvink
Karlsruhe Institute of Technology, Institute of Microstructure Technology, Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen, 76344 Germany
Search for more papers by this authorSummary
This chapter considers the hyphenation of small-scale sample preparation and/or separation techniques to nuclear magnetic resonance (NMR) spectroscopy. Four general modes of hyphenation are covered, namely: online continuous monitoring of column-based microseparations, online stopped-flow monitoring of column-based microseparations, off-line hyphenation of individual microsamples with microcoil detection, and integrated on-chip hyphenated microseparation/NMR detection. An essential component in the purification and analysis of unknown compounds is the efficient separation of individual components from an often complex chemical or biological mixture. The most common separation technique is high-pressure liquid chromatography (HPLC). The hyphenation of NMR detection to microfluidics can also be used to measure the reaction kinetics of very small amounts of material. In terms of future developments, the very rapid nature of chip-based electrophoretic separations may allow techniques such as hyperpolarization to be incorporated to increase the sensitivity; cryogenic radio-frequency (RF) coil technology at the microscale can also be anticipated to lead to substantial improvements in limits-of-detection.
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