Chapter 27
In Vitro Compartmentalization (IVC) and Other High-Throughput Screens of Enzyme Libraries
Amir Aharoni,
Dan S. Tawfik,
Amir Aharoni
Department of Life Science and the NIBN, University of Ben Gurion in the Negev, POB 653, Beer-Sheva, 84105, Israel
Search for more papers by this authorDan S. Tawfik
Department of Biological Chemistry, Weizmann Institute of Science, Ullmann Bldg, Room 201a, PO BOX 26, Rehovot 76100, Israel
Search for more papers by this authorAmir Aharoni,
Dan S. Tawfik,
Amir Aharoni
Department of Life Science and the NIBN, University of Ben Gurion in the Negev, POB 653, Beer-Sheva, 84105, Israel
Search for more papers by this authorDan S. Tawfik
Department of Biological Chemistry, Weizmann Institute of Science, Ullmann Bldg, Room 201a, PO BOX 26, Rehovot 76100, Israel
Search for more papers by this authorBook Editor(s):Prof. Dr. Stefan Lutz,
Prof. Dr. Uwe T. Bornscheuer,
Prof. Dr. Stefan Lutz
Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, USA
Search for more papers by this authorProf. Dr. Uwe T. Bornscheuer
Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany
Search for more papers by this authorFirst published: 12 November 2008
Summary
This chapter contains sections titled:
-
Introduction
-
The Fundamentals of High-Throughput Screens and Selections
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Enzyme Selections by Phage-Display
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HTS of Enzymes Using Cell-Display and FACS
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Other FACS-Based Enzyme Screens
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In vivo Genetic Screens and Selections
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In vitro Compartmentalization (IVC)
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IVC in Double Emulsions
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What's Next?
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Experimental Details
-
Acknowledgments
-
References
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