Chapter 13
Assessing and Exploiting the Persistence of Substrate Ambiguity in Modern Protein Catalysts
Kevin K. Desai,
Brian G. Miller,
Kevin K. Desai
Department of Chemistry and Biochemistry, The Florida State University, 213 Dittmer Laboratory, Tallahassee, FL 32306-4390, USA
Search for more papers by this authorBrian G. Miller
Department of Chemistry and Biochemistry, The Florida State University, 213 Dittmer Laboratory, Tallahassee, FL 32306-4390, USA
Search for more papers by this authorKevin K. Desai,
Brian G. Miller,
Kevin K. Desai
Department of Chemistry and Biochemistry, The Florida State University, 213 Dittmer Laboratory, Tallahassee, FL 32306-4390, USA
Search for more papers by this authorBrian G. Miller
Department of Chemistry and Biochemistry, The Florida State University, 213 Dittmer Laboratory, Tallahassee, FL 32306-4390, USA
Search for more papers by this authorBook Editor(s):Prof. Dr. Stefan Lutz,
Prof. Dr. Uwe T. Bornscheuer,
Prof. Dr. Stefan Lutz
Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, USA
Search for more papers by this authorProf. Dr. Uwe T. Bornscheuer
Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany
Search for more papers by this authorFirst published: 12 November 2008
Summary
This chapter contains sections titled:
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Quantitative Description of Enzyme Specificity
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Models of Enzyme Specificity
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Advantages and Disadvantages of Specificity
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Substrate Ambiguity as a Mechanism for Elaborated Metabolic Potential
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Experimental Approaches to Detect Ambiguity
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General Comments on Overexpression Libraries and Genetic Selections
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Challenges and Prospects for the Future
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References
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