This chapter contains sections titled:

• Introduction

• Materials and methods

  • Reference specimens

  • DB immunoassays

  • Antibody arrays at GNF

    • Antibodies, reagents, microarray printing, and platform

    • Microarray layout and processing

    • Array imaging and data analysis

  • Antibody microarrays at MSI

    • Chip manufacture

    • Rolling circle amplification (RCA) immunoassay

    • Conversion of mean fluorescent intensity to concentration

  • Antibody microarrays at VARI

    • Fabrication of antibody microarrays

    • Serum labeling

    • Processing of antibody microarrays

    • Analysis

  • Retrieval and matching of IPI numbers for the analytes

• Results

  • Antibody-based measurements of the HUPO reference specimens

  • Systematic variation between the preparation methods of the PPP reference specimens

  • Consistent alterations in specific protein abundances

  • Linkage of MS data and antibody-based measurements

• Discussion

• References