Chapter 19
Engraftment Analysis Using Short Tandem Repeats Following Allogeneic Hematopoietic Cell Transplantation
Ilka Warshawsky MD, PhD,
Hyun-Sook Chi MD, PhD,
Ilka Warshawsky MD, PhD
Department of Molecular Pathology, Cleveland Clinic Foundation, Cleveland, OH, USA
Search for more papers by this authorHyun-Sook Chi MD, PhD
University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea
Search for more papers by this authorIlka Warshawsky MD, PhD,
Hyun-Sook Chi MD, PhD,
Ilka Warshawsky MD, PhD
Department of Molecular Pathology, Cleveland Clinic Foundation, Cleveland, OH, USA
Search for more papers by this authorHyun-Sook Chi MD, PhD
University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea
Search for more papers by this authorBook Editor(s):Kandice Kottke-Marchant MD, PhD,
Bruce H. Davis MD,
Kandice Kottke-Marchant MD, PhD
Pathology & Laboratory Medicine Institute, Cleveland, OH, USA
Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA
Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA
Search for more papers by this authorFirst published: 10 April 2012
Summary
Allogeneic hematopoietic cell transplantation is used to treat a variety of malignant and nonmalignant hematologic disorders. Chimerism analysis is an important tool for monitoring post-transplant outcome. Chimerism testing allows for documentation of successful engraftment and ablation of recipient cells. It also predicts untoward events including disease relapse, graft rejection, and graft-versus-host disease so that preemptive immunotherapy can be instituted and responses to treatment monitored. The most commonly used approach for chimerism testing is PCR amplification of microsatellites or short tandem repeat (STR) loci. This approach relies on differences between donor and recipient polymorphic genetic markers. This chapter focuses on STR-PCR, an informative and sensitive method to accurately quantitate amounts of donor and recipient DNA following allogeneic hematopoietic cell transplantation.
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