Basement Membrane Protein BM-40
Erhard Hohenester
Imperial College, Department of Biological Sciences, London, UK
Search for more papers by this authorRupert Timpl
Max-Planck-Institut für Biochemie, Martinsried, Germany
Search for more papers by this authorErhard Hohenester
Imperial College, Department of Biological Sciences, London, UK
Search for more papers by this authorRupert Timpl
Max-Planck-Institut für Biochemie, Martinsried, Germany
Search for more papers by this authorAbstract
BM-40, also known as SPARC or osteonectin, is a secreted glycoprotein present in all metazoa. The protein is expressed in tissues undergoing morphogenesis, remodeling, and repair. In vitro, BM-40 binds to several collagens and is both antiproliferative and counteradhesive. Human BM-40 consists of an acidic N-terminal domain, a follistatin-like (FS) domain, and an α-helical (EC) domain that contains two functional EF-hand calcium-binding motifs. The crystal structure of the FS–EC domain pair has been determined. Calcium and collagen binding to the EC domain has been studied using site-directed mutagenesis, spectroscopy, and binding assays.
3D Structure
Schematic representation of the structure of the BM-40 FS–EC domain pair containing two calcium ions, PDB code 1BMO.35 The FS and EC domains are in green and blue respectively. Disulfide bridges are shown as yellow sticks. Calcium ions are shown as pink spheres. Made with BOBSCRIPT38 and RASTER3D.39
References
- 1 M Dziadek, M Paulsson, M Aumailley and R Timpl, Eur J Biochem, 161, 455–64 (1986).
- 2 JD Termine, HK Kleinman, SW Whitson, KM Conn, ML McGarvey and GR Martin, Cell, 26, 99–105 (1981).
- 3 IJ Mason, A Taylor, JG Williams, H Sage and BLM Hogan, EMBO J, 5, 1465–72 (1986).
- 4 RO Hynes and Q Zhao, J Cell Biol, 150, F89–F95 (2000).
- 5 Q Yan and EH Sage, J Histochem Cytochem, 47, 1495–505 (1999).
- 6 RA Brekken and EH Sage, Matrix Biol, 19, 569–80 (2000).
- 7 AD Bradshaw and EH Sage, J Clin Invest, 107, 1049–54 (2001).
- 8 L Purcell, J Guia–Gray, S Scanga and M Ringuette, J Exp Zool, 265, 153–64 (1993).
- 9 MC Fitzgerald and JE Schwarzbauer, Curr Biol, 8, 1285–88 (1998).
- 10 DT Gilmour, GJ Lyon, MB Carlton, JR Sanes, MJ Cunningham, JR Anderson, BL Hogan, MJ Evans and WH Colledge, EMBO J, 17, 1860–70 (1998).
- 11 K Norose, JI Clark, NA Syed, A Basu, S Heber–Katz, EH Sage and CC Howe, Invest Opthalmol Vis Sci, 39, 2674–80 (1998).
- 12 B Lankat–Buttgereit, K Mann, R Deutzmann, R Timpl and T Krieg, FEBS Lett, 236, 352–56 (1988).
- 13 A Swaroop, BLM Hogan and U Francke, Genomics, 2, 37–47 (1988).
- 14 XC Villarreal, KG Mann and GL Long, Biochemistry, 28, 6483–91 (1989).
- 15 K Mann, R Deutzmann, M Paulsson and R Timpl, FEBS Lett, 218, 167–72 (1987).
- 16 S Damjanovski, F Liu and MJ Ringuette, Biochem J, 281, 513–17 (1992).
- 17 MD Adams, JC Venter, et al., Science, 287, 2185–95 (2000).
- 18 JE Schwarzbauer and CS Spencer, Mol Biol Cell, 4, 941–52 (1993).
- 19 RW Romberg, PG Werness, P Lollar, BL Biggs and KG Mann, J Biol Chem, 260, 2728–36 (1985).
- 20 H Sage, B Vernon, SE Funk, EA Everitt and J Agnello, J Cell Biol, 109, 341–56 (1989).
- 21 RJ Kelm Jr and KG Mann, Blood, 75, 1105–13 (1990).
- 22 R Nischt, J Pottgieser, T Krieg, U Mayer, M Aumailley and R Timpl, Eur J Biochem, 200, 529–36 (1991).
- 23 J Pottgiesser, P Maurer, U Mayer, R Nischt, K Mann, R Timpl, T Krieg and J Engel, J Mol Biol, 238, 563–74 (1994).
- 24 P Maurer, C Hohenadl, E Hohenester, W Göhring, R Timpl and J Engel, J Mol Biol, 253, 347–57 (1995).
- 25 E Busch, E Hohenester, R Timpl, M Paulsson and P Maurer, J Biol Chem, 275, 25508–15 (2000).
- 26 JA Bassuk, LP Braun, K Motamed, F Baneyx and EH Sage, Int J Biochem Cell Biol, 28, 1031–43 (1996).
- 27 AD Bradshaw, JA Bassuk, A Francki and EH Sage, Mol Cell Biol Res Commun, 3, 345–51 (2000).
- 28 RJ Kelm and KG Mann, J Biol Chem, 266, 9632–39 (1991).
- 29 T Sasaki, W Göhring, K Mann, P Maurer, E Hohenester, V Knäuper, G Murphy and R Timpl, J Biol Chem, 272, 9237–43 (1997).
- 30 T Sasaki, N Miosge and R Timpl, Matrix Biol, 18, 499–508 (1999).
- 31 J Engel, W Taylor, M Paulsson, H Sage and B Hogan, Biochemistry, 26, 6958–65 (1987).
- 32 P Maurer, U Mayer, M Bruch, P Jenö, K Mann, R Landwehr, J Engel and R Timpl, Eur J Biochem, 205, 233–40 (1992).
- 33 E Hohenester, P Maurer, C Hohenadl, R Timpl, JN Jansonius and J Engel, Nat Struct Biol, 3, 67–73 (1996).
- 34 U Mayer, M Aumailley, K Mann, R Timpl and J Engel, Eur J Biochem, 198, 141–50 (1991).
- 35 E Hohenester, P Maurer and R Timpl, EMBO J, 16, 3778–86 (1997).
- 36 T Sasaki, E Hohenester, W Göhring and R Timpl, EMBO J, 17, 1625–34 (1998).
- 37 W Bode and R Huber, Eur J Biochem, 204, 433–51 (1991).
- 38 RM Esnouf, J Mol Graph, 15, 132–34 (1997).
- 39 EA Merritt and DJ Bacon, Methods Enzymol, 277, 505–24 (1997).
- 40 RH Kretsinger and CE Nockolds, J Biol Chem, 248, 3313–26 (1973).
- 41 NCJ Strynadka and MNG James, Annu Rev Biochem, 58, 951–98 (1989).
- 42 S Nakayama and RH Kretsinger, Annu Rev Biophys Biomol Struct, 23, 473–507 (1994).
- 43 A Lewit–Bentley and S Rety, Curr Opin Struct Biol, 10, 637–43 (2000).
- 44 P Maurer and E Hohenester, Matrix Biol, 15, 569–80 (1997).
- 45 R-L Xie and GL Long, J Biol Chem, 270, 23212–17 (1995).
- 46 W Göhring, T Sasaki, CH Heldin and R Timpl, Eur J Biochem, 255, 60–66 (1998).
- 47 EW Raines, TF Lane, ML Iruela–Arispe, R Ross and EH Sage, Proc Natl Acad Sci USA, 89, 1281–85 (1992).
- 48 TF Lane and EH Sage, J Cell Biol, 111, 3065–76 (1990).
- 49 M-H Huynh, EH Sage and M Ringuette, Dev Growth Differ, 41, 407–18 (1999).
- 50 EH Sage, JA Bassuk, JC Yost, MJ Folkman and TF Lane, J Cell Biochem, 57, 127–40 (1995).
- 51 IG Johnston, T Paladino, JW Gurd and IR Brown, Neuron, 2, 165–76 (1990).
- 52 M Shibanuma, J Mashimo, A Mita, T Kuroki and K Nose, Eur J Biochem, 217, 13–19 (1993).
- 53 PM Alliel, J-P Perin and FJ Bonnet, Eur J Biochem, 214, 347–50 (1993).
- 54 C Vannahme, S Schübel, M Herud, S Gösling, H Hülsmann, M Paulsson, U Hartmann and P Maurer, J Neurochem, 73, 12–20 (1999).
- 55 U Hartmann and P Maurer, Matrix Biol, 20, 23–35 (2001).
- 56 C Vannahme, N Smyth, N Miosge, S Gösling, C Frie, M Paulsson, P Maurer and U Hartmann, J Biol Chem, 277, 37977–86 (2002).
- 57 E Kohfeldt, P Maurer, C Vannahme and R Timpl, FEBS Lett, 414, 557–61 (1997).
