Considerable progress has been achieved in our understanding of the complexities of ROS and NO interactions over the last decade. Accumulating literature evidences demonstrates that ROS and RNS play key roles as biological messengers, functioning together to facilitate appropriate responses to biotic and abiotic stresses. ROS/RNS interplay is also important in the regulation of plant development and in the regulation of a wide range of physiological responses to the environment. Such background allows us to summarize our current knowledge about emerging technologies for enhancing ROS/RNS homeostasis. Respectively, in this chapter are considered the possibilities to use nitric oxide donors for abiotic stress tolerance: drought, salt stress, extreme temperatures and heavy metal stress. The perspectives for nitric oxide donors in agriculture are considered in details, especially for controlling seed vigor, dormancy and ripening, their biotechnological applications in wound healing and post harvested shelf life. The ways for improvement of NO donor application are analyzed (co-application of nitric oxide donors with fertilizers and perspectives on the use of nanoparticles releasing nitric oxide in crop industry). Special attention is paid to transgenic approaches for regulation of oxidative stress and antioxidant defense systems and perspectives for regulation of microRNAs and nitric oxide crosstalk in stress tolerance in plants and related challenges dictated by the development of genome editing and synthetic biology.