Alternative Non-Agrobacterium Based Methods for Plant Transformation
Annual Plant Reviews Online 2018 Volume 1
Issue 3, November 2018
Dheeraj S. Rathore,
Ewen Mullins,
Dheeraj S. Rathore
Dept. Crop Science, Teagasc, Oak Park, Carlow, Ireland
Search for more papers by this authorEwen Mullins
Dept. Crop Science, Teagasc, Oak Park, Carlow, Ireland
Search for more papers by this authorDheeraj S. Rathore,
Ewen Mullins,
Dheeraj S. Rathore
Dept. Crop Science, Teagasc, Oak Park, Carlow, Ireland
Search for more papers by this authorEwen Mullins
Dept. Crop Science, Teagasc, Oak Park, Carlow, Ireland
Search for more papers by this authorAbstract
Plant breeding continues to play a crucial role in generating crops fit for purpose to tackle the increasing number of a biotic stressors that farmers face. While the majority of engineered varieties have been and continue to be generated through the use of Agrobacterium tumefaciens via Agrobacterium-mediated transformation (AMT), more recently, the potential of non-Agrobacterium systems is coming to the fore, specifically in regard to rhizobia such as Ensifer adhaerens, Ochrobactrum haywardense, Rhizobium etli, and the collective of strains termed Transbacter™. This article reviews the landscapes of non-Agrobacterium species from the perspective of crop improvement, highlighting the mechanisms they use to manipulate host genomes as the basis for their possible transinfection strategies. Recruiting enhanced versions of these non-Agrobacterium strains empowered with stealthy mechanisms to deliver DNA/proteins into plant cells provides the potential to make a positive contribution to enhancing existing breeding programmes.
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