Chapter 22
In Vitro Replication Models
Volker Lohmann,
Sandra Bühler,
Ralf Bartenschlager,
Volker Lohmann
Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany
Search for more papers by this authorSandra Bühler
Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany
Search for more papers by this authorRalf Bartenschlager
Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany
Search for more papers by this authorVolker Lohmann,
Sandra Bühler,
Ralf Bartenschlager,
Volker Lohmann
Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany
Search for more papers by this authorSandra Bühler
Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany
Search for more papers by this authorRalf Bartenschlager
Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany
Search for more papers by this authorBook Editor(s):Howard C. Thomas BSc, PhD, FRCP, FRCPath, FMedSci,
Anna S.F. Lok MD,
Stephen A. Locarnini MBBS, BSc(Hons), PhD, FRCPath,
Arie J. Zuckerman MD, DSc, FRCP, FRCPath, FMedSci,
Howard C. Thomas BSc, PhD, FRCP, FRCPath, FMedSci
Emeritus Professor of Hepatology, Department of Medicine, Imperial College London, London, UK
Search for more papers by this authorAnna S.F. Lok MD
Alice Lohrman Andrews Research Professor in Hepatology, Director of Clinical Hepatology, Professor of Internal Medicine, Associate Chair for Clinical Research, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, MI, USA
Search for more papers by this authorStephen A. Locarnini MBBS, BSc(Hons), PhD, FRCPath
Head, Research & Molecular Development, Victorian Infectious Diseases Reference Laboratory, Melbourne, VIC, Australia
Search for more papers by this authorArie J. Zuckerman MD, DSc, FRCP, FRCPath, FMedSci
Emeritus Professor of Medical Microbiology, Formerly Principal and Dean, Royal Free Hospital School of Medicine
Search for more papers by this authorFirst published: 26 July 2013
Summary
The hepatitis C virus (HCV) is a major causative agent of chronic liver disease. Studies of the HCV replication cycle have become possible with the advent of cell culture models. These include HCV pseudoparticles (HCVpp) that are most suitable to study viral entry, the HCV replicon system, and the HCVcc system, recapitulating the intracellular steps or the complete viral replication cycle, respectively. These culture systems enabled detailed studies of the HCV–host cell interaction and finally paved the way for the development of direct-acting antiviral drugs. This chapter provides an overview about the various HCVin vitro replication models and their application to study the various aspects of the viral replication cycle.
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