The Olavius algarvensis Metagenome Revisited: Lessons Learned from the Analysis of the Low-Diversity Microbial Consortium of a Gutless Marine Worm
Manuel Kleiner
Max Planck Institute for Marine Microbiology, Bremen, Germany
Search for more papers by this authorTanja Woyke
Department of Energy Joint Genome Institute, Walnut Creek, California, USA
Search for more papers by this authorCaroline Ruehland
Max Planck Institute for Marine Microbiology, Bremen, Germany
Search for more papers by this authorNicole Dubilier
Max Planck Institute for Marine Microbiology, Bremen, Germany
Search for more papers by this authorManuel Kleiner
Max Planck Institute for Marine Microbiology, Bremen, Germany
Search for more papers by this authorTanja Woyke
Department of Energy Joint Genome Institute, Walnut Creek, California, USA
Search for more papers by this authorCaroline Ruehland
Max Planck Institute for Marine Microbiology, Bremen, Germany
Search for more papers by this authorNicole Dubilier
Max Planck Institute for Marine Microbiology, Bremen, Germany
Search for more papers by this authorFrans J. de Bruijn
Laboratory of Plant Micro-organism Interaction, CNRS-INRA, Castanet Tolosan, France
Search for more papers by this authorSummary
This chapter contains sections titled:
-
Introduction
-
Method Discussion: Challenges of Analyzing Co-Occurring Symbionts in Very Small Hosts
-
Major Findings of the Study in Brief
-
The Metagenome Revisited
-
Conclusions
-
Internet Resources for the O. Algarvensis Metagenome
-
References
INTERNET RESOURCES FOR THE O. ALGARVENSIS METAGENOME
- Annotated metagenome on IMG/M: http://img.jgi. doe.gov/cgi-bin/m/main.cgi
- Binned and unbinned metagenomic scaffolds as. fasta files: http://genome.jgipsf.org/olaal/olaal.download. ftp.html
- Sequence reads in trace archive http://www.ncbi.nlm. nih.gov/Traces/trace.cgi?cmd=retrieve&val=ncbi_project_id%3D17779
- Project page on NCBI (the unbinned scaffold dataset (DS021618-DS022223) is incomplete on this website, all other datasets are complete): http://www.ncbi. nlm.nih.gov/bookshelf/br.fcgi?book=metagenomics &part=gutlessworm
- Metagenome on Camera (Project: Mediterranean Gutless Worm Metagenome): http://web.camera.calit2. net/cameraweb/gwt/org.jcvi.camera.web.gwt.down load.BrowseProjectsPage/BrowseProjectsPage.oa# ViewProject
- Metagenome on MG-RAST (metagenome ID: 44411 15.3): http://metagenomics.nmpdr.org/metagenomics. cgi
- REFERENCES
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403–410.
- Böck A, Forchhammer K, Heider J, Leinfelder W, Sawers G, et al. 1991. Selenocysteine: The 21st amino acid. Mol. Microbiol. 5: 515–520.
- Chapman J, Putnam N, Ho I, Rokhsar DS. JAZZ, a whole genome shotgun assembler. Unpublished results.
- Dattagupta S, Schaperdoth I, Montanari A, Mariani S, Kita N, et al. 2009. A novel symbiosis between chemoautotrophic bacteria and a freshwater cave amphipod. ISME J. 3: 935–943.
- Denef VJ, Kalnejais LH, Mueller RS, Wilmes P, Baker BJ, et al. 2010. Proteogenomic basis for ecological divergence of closely related bacteria in natural acidophilic microbial communities. Proc. Natl. Acad. Sci. USA 107: 2383–2390.
- Dick G, Andersson A, Baker B, Simmons S, Thomas B, et al. 2009. Community-wide analysis of microbial genome sequence signatures. Genome Biol. 10: R85.
- Dubilier N, Mulders C, Ferdelman T, de Beer D, Pernthaler A, et al. 2001. Endosymbiotic sulphate-reducing and sulphide-oxidizing bacteria in an oligochaete worm. Nature 411: 298–302.
-
Dubilier N, Blazejak A, R ühland C. 2006. Symbiosis between bacteria and gutless marine oligochaetes. InOvermann J, ed. Molecular Basis of Symbiosis. Berlin: Springer, pp. 251–275.
10.1007/3-540-28221-1_12 Google Scholar
- Dubilier N, Bergin C, Lott C. 2008. Symbiotic diversity in marine animals: The art of harnessing chemosynthesis. Nat. Rev. Microbiol. 6: 725–740.
- Duchaud E, Rusniok C, Frangeul L, Buchrieser C, Givaudan A, et al. 2003. The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens. Nat. Biotech. 21: 1307–1313.
- Giere O, Erséus C. 2002. Taxonomy and new bacterial symbioses of gutless marine Tubificidae (Annelida, Oligochaeta) from the Island of Elba (Italy). Org. Divers. Evol. 2: 289–297.
- Giere O, Erséus C, Stuhlmacher F. 1998. A new species of Olavius (Tubificidae) from the Algarve Coast in Portugal, the first east Atlantic gutless oligochaete with symbiotic bacteria. Zool. Anz. 237: 209–214.
- Gillan DC, Dubilier N. 2004. Novel epibiotic Thiothrix bacterium on a marine Amphipod. Appl. Environ. Microbiol. 70: 3772–3775.
- Graham J. 2001. Biological Centrifugation. Oxford: BIOS Scientific Publishers, Ltd.
- Graham JM, Rickwood D, eds. 1997. Subcellular Fractionation: A Practical Approach. New York: Oxford University Press.
- Hao B, Gong W, Ferguson TK, James CM, Krzycki JA, et al. 2002. A new UAG-encoded residue in the structure of a methanogen methyltransferase. Science 296: 1462–1466.
- Harmer TL, Rotjan RD, Nussbaumer AD, Bright M, Ng AW, et al. 2008. Free-living tube worm endosymbionts found at deep-sea vents. Appl. Environ. Microbiol. 74: 3895–3898.
- Ishoey T, Woyke T, Stepanauskas R, Novotny M, Lasken RS. 2008. Genomic sequencing of single microbial cells from environmental samples. Curr. Opin. Microbiol. 11: 198–204.
- Johnson PLF, Slatkin M. 2006. Inference of population genetic parameters in metagenomics: A clean look at messy data. Genome Res. 16: 1320–1327.
- Kislyuk A, Bhatnagar S, Dushoff J, Weitz J. 2009. Unsupervised statistical clustering of environmental shotgun sequences. BMC Bioinf. 10: 316.
- Lott C. Unpublished data.
- Ludwig W, Strunk O, Westram R, Richter L, Meier H, et al. 2004. ARB: A software environment for sequence data. Nucleic Acids Res. 32: 1363–1371.
- McFall-Ngai M. 2008. Are biologists in “future shock”? Symbiosis integrates biology across domains. Nat. Rev. Microbiol. 6: 789–792.
- McHardy AC, Martin HG, Tsirigos A, Hugenholtz P, Rigoutsos I. 2007. Accurate phylogenetic classification of variable-length DNA fragments. Nat. Methods 4: 63–72.
- Moya A, Pereto J, Gil R, Latorre A. 2008. Learning how to live together: Genomic insights into prokaryote-animal symbioses. Nat. Rev. Genet. 9: 218–229.
- Musat N, Halm H, Winterholler B, Hoppe P, Peduzzi S, et al. 2008. A single-cell view on the ecophysiology of anaerobic phototrophic bacteria. Proc. Natl. Acad. Sci. USA 105: 17861–17866.
- Nussbaumer AD, Fisher CR, Bright M. 2006. Horizontal endosymbiont transmission in hydrothermal vent tubeworms. Nature 441: 345–348.
- Polz MF, Distel DL, Zarda B, Amann R, Felbeck H, et al. 1994. Phylogenetic analysis of a highly specific association between ectosymbiotic, sulfur-oxidizing bacteria and a marine nematode. Appl. Environ. Microbiol. 60: 4461–4467.
- Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, et al. 2010. A human gut microbial gene catalogue established by metagenomic sequencing. Nature 464: 59–65.
- Relman DA. 2008. “Til death do us part”: Coming to terms with symbiotic relationships. Nat. Rev. Microbiol. 6: 721–724.
- Rickwood D, ed. 1992. Preparative Centrifugation: A Practical Approach. Oxford: Oxford University Press.
- Ruby EG. 2008. Symbiotic conversations are revealed under genetic interrogation. Nat. Rev. Microbiol. 6: 752–762.
- Ruehland C, Blazejak A, Lott C, Loy A, Erséus C, et al. 2008. Multiple bacterial symbionts in two species of co-occurring gutless oligochaete worms from Mediterranean sea grass sediments. Environ. Microbiol. 10: 3404–3416.
- Rusk N. 2009. Cheap third-generation sequencing. Nat. Methods 6: 244–244.
- Srinivasan G, James CM, Krzycki JA. 2002. Pyrrolysine encoded by UAG in Archaea: Charging of a UAG-decoding specialized tRNA. Science 296: 1459–1462.
- Strittmatter AW, Liesegang H, Rabus R, Decker I, Amann J, et al. 2009. Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide. Environ. Microbiol. 11: 1038–1055.
- Strous M, Pelletier E, Mangenot S, Rattei T, Lehner A, et al. 2006. Deciphering the evolution and metabolism of an anammox bacterium from a community genome. Nature 440: 790–794.
- Taylor JD, Glover EA. 2000. Functional anatomy, chemosymbiosis and evolution of the Lucinidae. InHarper EM, Taylor JD, Crame JA, eds. Evolutionary Biology of the Bivalvia. Bath, UK: Geological Society, pp. 207–225.
-
Thompson FL, Austin B, Swings J, eds. 2006. The Biology of Vibrios. Washington, DC: American Society for Microbiology.
10.1128/9781555815714 Google Scholar
- Touchon M, Rocha EPC. 2007. Causes of insertion sequences abundance in prokaryotic genomes. Mol. Biol. Evol. 24: 969–981.
- Tyson GW, Chapman J, Hugenholtz P, Allen EE, Ram RJ, et al. 2004. Community structure and metabolism through reconstruction of microbial genomes from the environment. Nature 428: 37–43.
- Woyke T, Teeling H, Ivanova NN, Huntemann M, Richter M, et al. 2006. Symbiosis insights through metagenomic analysis of a microbial consortium. Nature 443: 950–955.
- Zhang Y, Gladyshev VN. 2007. High content of proteins containing 21st and 22nd amino acids, selenocysteine and pyrrolysine, in a symbiotic deltaproteobacterium of gutless worm Olavius algarvensis. Nucleic Acids Res. 35: 4952–4963.