Thin-Layer Chromatography
Liquid Chromatography
Update based on the original article by Joseph Sherma, Encyclopedia of Analytical Chemistry, ©2000, John Wiley & Sons, Ltd.
Abstract
Thin-layer chromatography (TLC) is one of the important chromatographic techniques. Its main advantages are low cost and the possibility of analyzing a large number of samples simultaneously. TLC is a very useful, rapid, and inexpensive chromatographic method. It is especially suitable for screening tests, in which pretreatment of the analytes can be avoided, even with dirty samples. The thin-layer format provides a better arrangement for high sample throughput, flexible detection strategies, and a greater tolerance of samples with a high-matrix burden. Currently, more and more popular application of computer-controlled scanning instruments, automated sample application, and the large number of available image analysis software packages allow high accuracy and precision in TLC quantification. Modern planar chromatographic techniques can achieve high separating power by use of two-dimensional (2-D) techniques, chromatographic plates with different properties, a variety of mobile phase combinations, a variety of forced-flow techniques, and multiple development modes.
Further Reading
- K. Bauer, L. Gros, W. Sauer, Thin Layer Chromatography – An Introduction, EM Science, Darmstadt, 1991.
- G. Morlock (ed.), CAMAG Bibliography Service (CBS), published in March and April each year and available open access on the website of CAMAG, Muttenz, Switzerland. Contains articles describing recent examples of planar chromatography in practice, as well as abstracts of published literature classified in 38 sections including reviews and books, fundamentals and theory, general techniques, special techniques, and applications to 34 different compound types.
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- B. Fried, J. Sherma, Thin Layer Chromatography – Techniques and Applications, 4th edition, Marcel Dekker, New York, 1999.
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- J. Sherma, ‘Planar Chromatography’, Anal. Chem., 70, 7–26 (1998). (Previous biennial reviews of TLC by J. Sherma are contained in issue 12 of this journal each even-numbered year dating back to volume 42, 1970.)
- J. Sherma, B. Fried (eds), Handbook of Thin Layer Chromatography, 2nd edition, Marcel Dekker, New York, 1996.
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Citing Literature
Encyclopedia of Analytical Chemistry: Applications, Theory and Instrumentation
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