Role of Nucleic Acid and Protein Manipulation Technologies in High-throughput Structural Biology Efforts
Dr. David J. Aceti
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorPaul G. Blommel
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorProf. Yaeta Endo
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Ehime University, Department of Applied Chemistry, Faculty of Engineering and The Venture Business Laboratory, Matsuyama, Japan, 790-8577
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Ronnie O. Frederick
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Adrian D. Hegeman
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Won Bae Jeon
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- +1-608-262-3364 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorTodd L. Kimball
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorJason M. Lee
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Craig S. Newman
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Francis C. Peterson
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Medical College of Wisconsin, Department of Biochemistry, Milwaukee, Wisconsin, USA, 53226
Search for more papers by this authorDr. Tatsuya Sawasaki
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Ehime University, Department of Applied Chemistry, Faculty of Engineering and The Venture Business Laboratory, Matsuyama, Japan, 790-8577
Search for more papers by this authorKory D. Seder
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- +1-608-262-0254 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorProf. Michael R. Sussman
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- +1-608-262-8608 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Eldon L. Ulrich
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Russell L. Wrobel
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorSandy Thao
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Dmitriy A. Vinarov
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University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorProf. Brian F. Volkman
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- +1-414-456-8400 | Fax: +1-414-456-6510
Medical College of Wisconsin, Department of Biochemistry, Milwaukee, Wisconsin, USA, 53226
Search for more papers by this authorDr. Qin Zhao
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- +1-608-262-1754 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. David J. Aceti
- [email protected]
- +1-608-262-4687 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorPaul G. Blommel
- [email protected]
- +1-608-262-3364 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorProf. Yaeta Endo
- [email protected]
- +81-89-927-9936 | Fax: +81-608-262-3453
Ehime University, Department of Applied Chemistry, Faculty of Engineering and The Venture Business Laboratory, Matsuyama, Japan, 790-8577
Search for more papers by this authorProf. Brian G. Fox
- [email protected]
- +1-608-262-9708 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Ronnie O. Frederick
- [email protected]
- +1-608-262-4687 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Adrian D. Hegeman
- [email protected]
- +1-608-262-1779 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Won Bae Jeon
- [email protected]
- +1-608-262-3364 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorTodd L. Kimball
- [email protected]
- +1-608-262-4687 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorJason M. Lee
- [email protected]
- +1-608-262-4687 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Craig S. Newman
- [email protected]
- +1-608-262-3173 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Francis C. Peterson
- [email protected]
- +1-414-456-8081 | Fax: +1-414-456-6510
Medical College of Wisconsin, Department of Biochemistry, Milwaukee, Wisconsin, USA, 53226
Search for more papers by this authorDr. Tatsuya Sawasaki
- [email protected]
- +81-89-927-9936 | Fax: +81-608-262-3453
Ehime University, Department of Applied Chemistry, Faculty of Engineering and The Venture Business Laboratory, Matsuyama, Japan, 790-8577
Search for more papers by this authorKory D. Seder
- [email protected]
- +1-608-262-0254 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorProf. Michael R. Sussman
- [email protected]
- +1-608-262-8608 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Eldon L. Ulrich
- [email protected]
- +1-608-265-5741 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Russell L. Wrobel
- [email protected]
- +1-608-262-9708 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorSandy Thao
- [email protected]
- +1-608-262-3364 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorDr. Dmitriy A. Vinarov
- [email protected]
- +1-608-264-0459 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorProf. Brian F. Volkman
- [email protected]
- +1-414-456-8400 | Fax: +1-414-456-6510
Medical College of Wisconsin, Department of Biochemistry, Milwaukee, Wisconsin, USA, 53226
Search for more papers by this authorDr. Qin Zhao
- [email protected]
- +1-608-262-1754 | Fax: +1-608-265-3453
University of Wisconsin, Department of Biochemistry, College of Agricultural and Life Sciences, Madison, USA, 53706
Search for more papers by this authorAbstract
- Introduction
- Overview of Structural Genomics Initiatives World-wide
- Use of Bioinformatics as a Predictive Tool
- High-throughput Cloning Strategies Applied to Arabidopsis
- cDNA Sources
- DNA Chip Technologies as a Predictive Tool
- Genes Lacking Introns
- Restriction Digestion and Ligation
- Topoisomerase Cloning
- Transformation
- Sequence Verification
- Escherichia coli Expression Systems
- T7 Promoter Systems
- Arabinose Operon Promoter System
- Wheat Germ Cell-free Translation Systems
- Characterization of Expression, Solubility, and Purity
- Electrophoresis and Dye Binding
- ELISA-based Detection
- S-Tag-Based Detection
- Protein Tags and Multi-step Purification Procedures
- Role of Protease Cleavage in Target Preparation
- Protein Characterization by Mass Spectrometry
- Integration with Broader Biological Sciences Community
- Acknowledgments
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