Bioemulsans: Surface-active Polysaccharide-containing Complexes
Part 5. Polysaccharides
Prof. Dr. Eugene Rosenberg,
Prof. Dr. Eliora Z. Ron,
Prof. Dr. Eugene Rosenberg
- [email protected]
- +972-3-640 9838 | Fax: +972-3-642 9377
Tel Aviv University, Department of Molecular Microbiology and Biotechnology, Ramat Aviv, Israel, 69978
Search for more papers by this authorProf. Dr. Eliora Z. Ron
- [email protected]
- +972-3-640 9879 | Fax: +972-3-641 4138
Tel Aviv University, Department of Molecular Microbiology and Biotechnology, Ramat Aviv, Israel, 69978
Search for more papers by this authorProf. Dr. Eugene Rosenberg,
Prof. Dr. Eliora Z. Ron,
Prof. Dr. Eugene Rosenberg
- [email protected]
- +972-3-640 9838 | Fax: +972-3-642 9377
Tel Aviv University, Department of Molecular Microbiology and Biotechnology, Ramat Aviv, Israel, 69978
Search for more papers by this authorProf. Dr. Eliora Z. Ron
- [email protected]
- +972-3-640 9879 | Fax: +972-3-641 4138
Tel Aviv University, Department of Molecular Microbiology and Biotechnology, Ramat Aviv, Israel, 69978
Search for more papers by this authorAbstract
- Introduction
- Historical Outline
- The Petroleum Industry Connection
- The Emulsan Story
- Occurrence and Chemical Properties
- RAG-1 Emulsan
- BD4 Emulsan
- Alasan
- Biodispersan
- Other High Molecular-mass Bioemulsifiers
- Natural Role of Emulsans
- Increasing the Surface Area of Hydrophobic Water-insoluble Substrates
- Increasing the Bioavailability of Hydrophobic Water-insoluble Substrates
- Binding of Toxic Heavy Metals
- Regulating the Attachment-detachment of Microorganisms to and from Surfaces
- Genetics and Regulation of Bioemulsan Production
- Biodegradation
- Production
- Shake-flask Experiments
- Production in Small Fermentors
- Large-scale Production of Emulsans
- Patents
- Potential Applications
- Outlook and Perspectives
References
- Abbot, B. J., Gledhill, W. E. (1971) The extracellular accumulation of metabolic products by hydrocarbon-degrading microorganisms, Adv. Appl. Microbiol. 14, 249–388.
- Appaiah, A. K. A., Karanth, N. G. K. (1991) Insecticide specific emulsifier production by hexachlorocyclohexane-utilizing Pseudomonas tralucida Ptm+ strain, Biotechnol. Lett. 13, 371–374.
- Ashtaputre, A. A., Shah, A. K. (1995) Emulsifying property of a viscous exopolysaccharide from Sphingomonas paucimobilis, World J. Microbiol. Biotechnol. 11, 219–222.
- Banat, I. M. (1995) Biosurfactants production and possible use in microbial enhanced oil recovery and oil pollution remediation: a review, Biosource Technol. 51, 1–12.
- Barkay, T., Navon-Venezia, S., Ron, E. Z., Rosenberg, E. (1999) Enhancement of solubilization and biodegradation of polyaromatic hydrocarbons by the bioemulsifier alasan, Appl. Environ. Microbiol. 65, 2697–2702.
- Beebe, J. L., Umbreit, W. W. (1971) Extracellular lipid of Thiobacillus thiooxidans, J Bacteriol. 108, 612–615.
- Belsky, I., Gutnick, D. L., Rosenberg, E. (1979) Emulsifier of Arthrobacter RAG-1: determination of emulsifier-bound fatty acids, FEBS Lett. 101, 175–178.
- Bohringer, J., Fischer, D., Mosler, G., Hengge-Aronis, R. (1995) UDP-glucose is a potential intracellular signal molecule in the control of expression of sigma S and sigma S-dependent genes in Escherichia coli, J. Bacteriol. 177, 413–422.
- Bredholt, H., Eimhjellen, K. (1999) Induction and development of the oil emulsifying system in an alkane oxidizing Rhodococcus species, Can. J. Microbiol. 45, 700–708.
- Brown, W. A., Cooper, D. G. (1991) Self-cycling fermentation applied to Acinetobacter calcoaceticus RAG-1, Appl. Environ. Microbiol. 57, 2901–2906.
- Bunster, L., Fokkema, N. J., Shippers, B. (1989) Effect of surface-active Pseudomonas spp. on leaf wettability, Appl. Environ. Microbiol. 55, 1434–1435.
- Calvo, C., Martinez-Checa, F., Mota, A., Bejar, V., Quesada, E. (1998) Effect of cations, pH and sulfate content on the viscosity and emulsifying activity on the Halomonas eurihalina, J. Ind. Microbiol. Biotechnol. 20, 205–209.
- Cameron, D. R., Cooper, D. G., Neufeld, R. J. (1988) The mannoprotein of Saccharomyces cerevisiae is an effective bioemulsifier, Appl. Environ. Microbiol. 54, 1420–1425.
- Choi, J. W., Choi, H. G., Lee, W. H. (1996) Effects of ethanol and phosphate on emulsan production by Acinetobacter calcoaceticus RAG-1, J. Biotechnol. 45, 217–225.
- Cirigliano, M. C., Carman, G. M. (1984) Purification and characterization of liposan, a bioemulsifier from Candida lipolytica, Appl. Environ. Microbiol. 50, 846–850.
- Dahan, O. (1998) Isolation and characterization of alasan mutants in Acinetobacter radioresistens, MSc Thesis, Tel Aviv University.
- Davis, J. B. (1967) Petroleum Microbiology, New York: Elsevier.
- De Acevedo, G. T., McInerney, M. J. (1996) Emulsifying activity in thermophilic and extremely thermophilic microorganisms, J. Ind. Microbiol. 16, 17–22.
- Desai, J., Banat, I. (1997) Microbial production of surfactants and their commercial potential, Microbiol. Mol. Biol. R. 61, 47–48.
- Deziel, E., Paquette, G., Villemur, R., Lepine, F., Bisaillon, J. G. (1996) Biosurfactant production by a soil Pseudomonas strain growing on polycyclic aromatic hydrocarbons, Appl. Environ. Microbiol. 62, 1908–1912.
- Dostalek, M., Spurny, M. (1958) Bacterial release of oil, I. Folia Biol. (Prague), IV, 166–171.
- Elkeles, A., Rosenberg, E., Ron, E. Z. (1994) Production and secretion of the polysaccharide biodispersan of Acinetobacter calcoaceticus A1 in protein secretion mutants, Appl. Environ. Microbiol. 60, 4642–4645.
- Erickson, L. E., Nakahara, T. (1975) Growth in cultures with two liquid phases: hydrocarbon uptake and transport, Process Biochem. 10, 9–13.
- Fiechter, A. (1992) Biosurfactants: moving towards industrial application, Trends Biotechnol. 10, 208–217.
- Fraenkel, D. G. (1992) Genetics and intermediary metabolism, Annu. Rev. Genet. 26, 159–177.
-
Goldberg, I.
(1985)
Single Cell Protein, Heidelberg: Springer-Verlag.
10.1007/978-3-642-46540-6 Google Scholar
- Goldenberg-Dvir, V. (1998) A new bioemulsifier produced by the oil-degrading Acinetobacter junii V-26, MSc thesis, Tel-Aviv University.
- Goldman, S., Shabtai, Y., Rubinovitz, C., Rosenberg, E., Gutnick, D. L. (1982) Emulsan in Acinetobacter calcoaceticus RAG-1: distribution of cell-free and cell-associated cross-reacting materials, Appl. Environ. Microbiol. 44, 165–170.
- Gunjar, M., Khire, J. M., Khan, M. I. (1995) Bioemulsifier production by Bacillus stearothermophilus VR8 isolate, Lett. Appl. Microbiol. 21, 83–86.
- Gutnick, D. L., Rosenberg, E. (1977) Oil tankers and pollution: a microbiological approach, Annu. Rev. Microbiol. 31, 379–396.
- Iguchi, T., Takeda, I., Ohsawa, H. (1969) Emulsifying factor of hydrocarbon produced by a hydrocarbon-assimilating yeast, Agric. Biol. Chem. 33, 1657–1658.
- Johnson, M. J. (1968) Utilization of hydrocarbon by microorganisms, Chem. Ind. (London) 36, 1532–1537.
- Kaplan, N., Rosenberg, E. (1982) Exopolysaccharide distribution and bioemulsifier production in Acinetobacter calcoaceticus BD4 and BD413, Appl. Environ. Microbiol. 44, 1335–1341.
- Kaplan, N., Jann, B., Jann, K. (1985) Structural studies on the capsular polysaccharide of Acinetobacter calcoaceticus BD4, Eur. J. Biochem. 152, 453–458.
- Kaplan, N., Zosim, Z., Rosenberg, E. (1987) Acinetobacter calcoaceticus BD4 emulsan: reconstitution of emulsifying activity with pure polysaccharide and protein, Appl. Environ. Microbiol. 53, 440–446.
- Kaeppeli, O., Walther, P., Mueller, M., Fiechter, A. (1984) Structure of cell surface of the yeast Candida tropicalis and its relation to hydrocarbon transport, Arch. Microbiol. 138, 279–282.
- La Riviere, J. W. M. (1955a) The production of surface active compounds by microorganisms and its possible significance in oil recovery. I. Some general observations on the change of surface tension in microbial cultures, Antonie van Leeuwenhoek, J. Microbiol. Serol. 21, 1–8.
- La Riviere, J. W. M. (1955b) The production of surface active compounds by microorganisms and its possible significance in oil recovery. II. On the release of oil from oil-sand mixtures with the aid of sulphate-reducing bacteria, Antonie van Leeuwenhoek, J. Microbiol. Serol. 21, 9–16.
- Marin, M., Pedregosa, A., Laborda, F. (1996) Emulsifier production and microscopical study of emulsions and biofilms formed by the hydrocarbon-utilizing bacteria Acinetobacter calcoaceticus MM5, Appl. Microbiol. Biotechnol. 44, 660–667.
- Navon-Venezia, S., Zosim, Z., Gottlieb, A., Legmann, R., Carmeli, S., Ron, E. Z., Rosenberg, E. (1995) Alasan, a new bioemulsifier from Acinetobacter radioresistens, Appl. Environ. Microbiol. 61, 3240–3244.
- Navon-Venezia, S., Banin, E., Ron, E. Z., Rosenberg, E. (1998) The bioemulsifier alasan: role of protein in maintaining structure and activity, Appl. Microbiol. Biotechnol. 49, 382–384.
- Neu, T. R. (1996) Significance of bacterial surface-active compounds in interaction of bacteria with interfaces, Microbiol. Rev. 60, 151–166.
- Neu, T. R., Poralla, K. (1990) Emulsifying agent from bacteria isolated during screening for cells with hydrophobic surfaces, Appl. Microbiol. Biotechnol. 32, 521–525.
- Neufeld, R. J., Zajic, J. E. (1984) The surface activity of Acinetobacter calcoaceticus sp. 2CA2, Biotechnol. Bioeng. 26, 1108–1114.
- Pines, O., Bayer, E.A., Gutnick, D. L. (1983) Localization of emulsan-like polymers associated with the cell surface of Acinetobacter calcoaceticus, J. Bacteriol. 154, 893–905.
- Pines, P., Gutnick, D. L. (1981) Relationship between phase resistance and emulsan production, interaction of phases with the cell-surface of Acinetobacter calcoaceticus, Arch. Microbiol. 130, 129–133.
- Reisfeld, A., Rosenberg, E., Gutnick, D. (1972) Microbial degradation of crude oil: factors affecting the dispersion in sea water by mixed and pure cultures, Appl. Environ. Microbiol. 24, 363–368.
- Ron, E.Z., Rosenberg, E. (2001) Natural roles of biosurfactants, Environ. Microbiol. 3, 229–236.
- Rosenberg, E., Kaplan, N. (1987) Surface-active properties of Acinetobacter expolysaccharides, in: Bacterial Outer Membranes as Model Systems ( M. Inouye, Ed.), New York: John Wiley & Sons, 311–342.
- Rosenberg, E., Ron, E. Z. (1990) Bacterial process for the production of biodispersants. Israel-Patent Application No. 76981.
-
Rosenberg, E.,
Ron, E. Z.
(1996)
Bioremediation of petroleum contamination, in: Bioremediation: Principles and Applications ( R. L. Crawford, D. L. Crawford, Eds.), Cambridge University Press,
100–124.
10.1017/CBO9780511608414.006 Google Scholar
- Rosenberg, E., Ron, E. Z (1997) Bioemulsans: microbial polymeric emulsifiers, Curr. Opin. Biotechnol. 8, 313–316.
-
Rosenberg, E.,
Ron, E. Z.
(1998a)
Surface active polymers from the genus Acinetobacter, in: Biopolymers from Renewable Resources ( D. L. Kaplan, Ed.), New York: Springer-Verlag,
281–291.
10.1007/978-3-662-03680-8_11 Google Scholar
- Rosenberg, E., Ron, E. Z. (1998b) Novel bioemulsifiers. Israeli Patent Application No. 112,254, 5/1/95: U.S. Patent No. 5,840,547 (November 24, 1998).
- Rosenberg, E., Ron, E. Z. (1999) . High and low molecular mass microbial surfactants, Appl. Microbiol. Biotechnol. 52, 154–162.
- Rosenberg, E., Horowitz, A., Englander, E., Gutnick, D. L. (1974) Bacterial emulsion of crude oil in seawater, Symposium on Impact of Microorganisms on the Aquatic Environment, USEPA Publication, 157–168.
- Rosenberg, E., Perry, A., Gibson, D. T., Gutnick, D. (1979a) Emulsifier of Arthrobacter RAG-1: specificity of hydrocarbon substrate, Appl. Environ. Microbiol. 37, 409–413.
- Rosenberg, E., Zuckerberg, A., Rubinovitz, C., Gutnick, D. L. (1979b) Emulsifier of Arthrobacter RAG-1: Isolation and emulsifying properties, Appl. Environ. Microbiol. 37, 402–408.
- Rosenberg, E., Gottlieb, A., Rosenberg, M. (1983) Inhibition of bacterial adherence to hydrocarbons and epithelial cells by emulsan, Infect. Immun. 39, 1024–1028.
- Rosenberg, E., Rubinovitz, C., Gottlieb, A., Rosenhak, S., Ron, E. Z. (1988a) Production of biodispersan by Acinetobacter calcoaceticus A2, Appl. Environ. Microbiol. 54, 317–322.
- Rosenberg, E., Rubinovitz, C., Legmann, R., Ron, E. Z. (1988b) Purification and chemical properties of Acinetobacter calcoaceticus A2 biodispersan, Appl. Environ. Microbiol. 54, 323–326.
- Rosenberg, E., Schwartz, Z., Tenenbaum, A., Rubinovitz, C., Legmann, R., Ron, E. Z. (1989) A microbial polymer that changes the surface properties of limestone: effect of biodispersan in grinding limestone and making paper, J. Dispersion Sci. Technol. 10, 241–250.
- Rosenberg, E., Barkay, T., Navon-Venezia, S., Ron, E. Z. (1999) Role of Acinetobacter bioemulsans in petroleum degradation, in: Novel Approaches for Bioremediation of Organic Pollution ( R. Fass, Y. Flasher, Eds), New York: Kluwer Academic/Plenum Publishers.
- Rubinovitz, C., Gutnick, D. L., Rosenberg, E. (1982) Emulsan production by Acinetobacter calcoaceticus in the presence of chloramphenicol, J. Bacteriol. 152, 126–132
- Sar, N., Rosenberg, E. (1983) Emulsifier production by Acinetobacter calcoaceticus strains, Curr. Microbiol. 9, 309–314.
- Shabtai, Y., Gutnick, D. L. (1985) Exocellular esterase and emulsan release from the cell surface of Acinetobacter calcoaceticus, J. Bacteriol. 161, 1176–1181.
- Shepard, R., Rockey, J., Sutherland, I. W., Roller, S. (1995) Novel bioemulsifiers from microorganisms for use in foods, J. Biotechnol. 40, 207–217.
- Shoham, Y., Rosenberg, E. (1983) Enzymatic depolymerization of emulsan, J. Bacteriol. 156, 161–167.
- Shoham, Y., Rosenberg, M., Rosenberg, E. (1983) Bacterial degradation of emulsan, Appl. Environ. Microbiol. 46, 573–579.
- Stark, M. (1996) Analysis of the exopolysaccharide gene cluster from Acinetobacter calcoaceticus BD4, PhD thesis, Tel-Aviv University.
- Sullivan, E. R. (1998) Molecular genetics of biosurfactant production, Curr. Opin. Biotechnol. 9, 263–269.
- Taylor, W. H., Juni, E. (1961) Pathways for biosynthesis of a bacterial capsular polysaccharide. I. Characterization of the organism and polysaccharide, J. Bacteriol. 81, 688–693.
- Toren, A., Navon-Venezia, S., Ron, E. Z., Rosenberg, E. (2001) Emulsifying activities of purified alasan proteins from Acinetobacter radioresistens KA53, Appl. Environ. Microbiol. 67, 1102–1106.
- Updegraff, D. M., Wren, G. B. (1954) The release of oil from petroleum bearing materials by sulfate reducing bacteria, Appl. Microbiol. 2, 309–316.
- Van Delden, C., Pesci, E. C., Pearson, J. P., Iglewski, B. H. (1998) Starvation selection restores elastase and rhamnolipid production in a Pseudomonas aeruginosa quorum-sensing mutant, Infect. Immun. 66, 4499–4502.
- Wang, S. D., Wand, D. I. C. (1990) Mechanisms for biopolymer accumulation in immobilized Acinetobacter calcoaceticus system, Biotechnol. Bioeng. 36, 402–410.
- Zajic, J. E., Panchal, C. J. (1976) Bioemulsifiers, CRC Crit. Rev. Microbiol. 5, 39–66.
- Zajic, J. E., Guignard, H., Gerson, D. F. (1977) Properties and biodegradation of a bioemulsifier from Corynebacterium hydrocarboclastus, Biotechnol. Bioeng. 19, 1303–1320.
- Zhang, Y., Miller, R. M. (1994) Effect of a Pseudomonas rhamnolipid biosurfactant on cell hydrophobicity and biodegradation of octadecane, Appl. Environ. Microbiol. 60, 2101–2106.
- Zosim, Z., Gutnick, D. L., Rosenberg, E. (1983) Uranium binding by emulsan and emulsanosols, Biotech. Bioeng. 25, 1725–1735.
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