In vitro Biosynthesis of Polyhydroxyalkanoates
Dr. Shiming Zhang
- [email protected]
- +01-413-577-3139 | Fax: +01-413-545-1578
University of Massachusetts, Department of Microbiology, Amherst, MA, USA, 01003
Search for more papers by this authorProf. Dr. Robert W. Lenz
- [email protected]
- +01-413-545-3060 | Fax: +01-413-545-2595
University of Massachusetts, Polymer Science and Engineering Department, Amherst, MA, USA, 01003
Search for more papers by this authorProf. Dr. Steve Goodwin
- [email protected]
- +01-413-545-4604 | Fax: +01-413-545-1578
University of Massachusetts, Department of Microbiology, Amherst, MA, USA, 01003
Search for more papers by this authorDr. Shiming Zhang
- [email protected]
- +01-413-577-3139 | Fax: +01-413-545-1578
University of Massachusetts, Department of Microbiology, Amherst, MA, USA, 01003
Search for more papers by this authorProf. Dr. Robert W. Lenz
- [email protected]
- +01-413-545-3060 | Fax: +01-413-545-2595
University of Massachusetts, Polymer Science and Engineering Department, Amherst, MA, USA, 01003
Search for more papers by this authorProf. Dr. Steve Goodwin
- [email protected]
- +01-413-545-4604 | Fax: +01-413-545-1578
University of Massachusetts, Department of Microbiology, Amherst, MA, USA, 01003
Search for more papers by this authorAbstract
- Introduction
- Historical Outline
- Characterization of PHA Synthase Activity
- Isolation and Activity of Soluble PHA Synthase
- Properties of PHA Synthases
- Lag Phase in Polymerization Reaction
- Elimination of Lag Phase
- Substrate Specificity
- Mechanism of Polymerization Reaction
- In vitro Polymerization of HACoA Monomers
- Control of Polymer Properties
- Molecular Weight
- Repeating Unit Distribution
- Molecular Weight Polydispersity
- Stabilizing of PHA Synthase Activity
- New Types of PHAs
- Diversity of PHA Synthases
- Control of Polymer Properties
- CoA Recycling Systems
- Patents
- Outlook and Perspectives
References
-
Ballard, D. G. H.,
Holmes, P. A.,
Senior, P. J.
(1987)
Formation of polymers of β-hydroxybutyric acid in bacterial cells and a comparison of the morphology of growth with the formation of polyethylene in the solid state. in: Recent Advances in Mechanistic and Synthesis Aspects of Polymerization, ( M. Fontanille and A. Guyot, Eds), Lancaster: Reidel (Kluwer),
293–314.
10.1007/978-94-009-3989-9_22 Google Scholar
- Doi, Y., Kawaguchi, Y., Koyama, N., Nakamura, S., Hiramitsu, M., Yoshida, Y., Kimura, H. (1992) Synthesis and degradation of polyhydroxyalkanoates in Alcaligenes eutrophus, FEMS Microbiol. Rev. 103, 103–108.
- Fukui, T., Yoshimoto, A., Matsumoto, M., Hosokawa, S., Saito, T., Nishikawa, H., Tomita, K. (1976) Enzymatic synthesis of poly-β-hydroxybutyrate in Zoogloea ramigera, Arch. Microbiol. 110, 149–156.
- Gerngross, T. U. (1999) Can biotechnology move us towards a sustainable future? Nat. Biotechnol. 17, 541–544.
- Gerngross, T. U., Martin, D. P. (1995) Enzyme-catalyzed synthesis of poly[(R)-(–)-3- hydroxybutyrate]: formation of macroscopic granules in vitro, Proc. Natl Acad. Sci. USA 92, 6279–6283.
- Gerngross, T. U., Snell, K. D., Peoples, O. P., Sinskey, A. J., Csuhai, E., Masamune, S., Stubbe, J. (1994) Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity, Biochemistry 33, 9311–9320.
- Griebel, R. J., Merrick, J. M. (1971) Metabolism of poly-β-hydroxybutyrate: effect of mild alkaline extraction on native poly-β-hydroxybutyrate granules, J. Bacteriol. 108, 782–789.
- Griebel, R. J., Smith, Z., Merrick, J. M. (1968) Metabolism of poly-3-hydroxybutyrate. I. purification, composition, and properties of poly-3-hydroxybutyrate granules from Bacillus megaterium, Biochemistry 7, 3676.
- Haywood, G. W., Anderson, A. J., Dawes, E. A. (1989) The importance of PHB-synthase substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus, FEMS Microbiol. Lett. 57, 1–6.
- Hein, S., Tran, H., Steinbüchel, A. (1998) Synechocystis sp. PCC6903 posses a two component polyhydroxyalkanoate acid synthase similar to that of the anoxygenic purple sulfur bacteria, Arch. Microbiol. 170, 162–170.
- Jia, Y., Kappock, J., Frick, T., Sinskey, A. J., Stubbe, J. (2000) Lipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: characterization of the functional residues in Chromatium vinosum PHB synthase, Biochemistry 39, 3927–3936.
- Jossek, R., Steinbüchel, A. (1998) In vitro synthesis of poly(3-hydroxybutyric acid) by using an enzymatic coenzyme A recycling system, FEMS Microbiol. Lett. 168, 319–324.
- Jossek, R., Reichelt, R., Steinbüchel, A. (1998) In vitro biosynthesis of poly(3-hydroxybutyric acid) by using purified poly(hydroxyalkanoic acid) synthase of Chromatium vinosum, Appl. Microbiol. Biotechnol. 49, 258–266.
- Kamachi, M., Zhang, S., Goodwin, S., Lenz, R. W. (2001) Enzymatic polymerization and characterization of new poly(3-hydroxyalkanoates) by a bacterial polymerase, Macromolecules, in press.
- Kawaguchi, Y., Doi, Y. (1992) Kinetics and mechanism of synthesis and degradation of poly(3-hydroxybutyrate) in Alcaligenes eutrophus, Macromolecules 25, 2324–2329.
- Kraak, M. N., Kessler, B., Witholt, B. (1997) In vitro activities of granule-bound [(R)-3-hydroxyalkanoate] polymerase C1 of Pseudomonas oleovorans development of an activity test for medium-chain-length-poly(2-hydroxyalkanoate) polymerases, Eur. J. Biochem. 250, 432–439.
- Lee, S. Y., Choi, J., Wong, H. H. (1999) Recent advances in polyhydroxyalkanoate production by bacterial fermentation: mini-review, Int. J. Biol. Macromol. 25, 31–36.
- Lemoigne, M. (1925) Etudes sur l'autolyse microbie acidification par formation d'acide β-oxybutyrique, Ann. Inst. Pasteur 39, 144.
- Lenz, R. W., Farcet, C., Dijkstra, P. J., Goodwin, S., Zhang, S. (1999) Extracellular polymerization of 3-hydroxyalkanoate monomers with the polymerase of Alcaligenes eutrophus, Int. J. Biol. Macromol. 25, 55–60.
- Liebergesell, M., Schmidt, B., Steinbüchel, A. (1992) Isolation and identification of granule-associated proteins relevant for poly(3-hydroxyalkanoic acid) biosynthesis in Chromatium vinosum D, FEMS Microbiol. Lett. 99, 227–232.
- Liebergesell, M., Sonomoto, K., Madkour, M., Mayer, F., Steinbüchel, A. (1994) Purification and characterization of the poly(hydroxyalkanoic acid) synthase from Chromatium vinosum and localization of the enzyme at the surface of poly(hydroxyalkanoic acid) granules, Eur. J. Biochem. 226, 71–80.
- Liu, S.-J., Steinbüchel, A. (2000) Exploitation of butyrate kinase and phosphotransbutyrylase from Clostridium acetobutylicum for the in vitro biosynthesis of poly(hydroxyalkanoic acid), Appl. Microbiol. Biotechnol. 53, 545–552.
- Martin, D. P., Gerngross, T. U. (1996) Aspects of PHB formation in vitro. in: International Symposium on Bacterial Polyhydroxyalkanoates ( G. Eggink, A. Steinbüchel, Y. Poirier, A. Endo, Eds), Ottawa: NRC Research Press, 28–35.
- Merrick, J. M., Doudoroff, M. (1961) Enzymatic synthesis of poly-β-hydroxybutyric acid in bacteria, Nature 189, 890–892.
- Müh, U., Sinskey, A. J., Kirby, D. P., Lane, W. S., Stubbe, J. (1999) PHA synthase from Chromatium vinosum: cysteine 149 is involved in covalent catalysis, Biochem. 38, 826–837.
- Nawrath, C., Poirier, Y., Somerville, C. (1994) Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation, Proc. Natl. Acad. Sci. USA 91, 12760–12764.
- Nobes, G. A. R., Jurasek, L., Marchessault, R. H., Martin, D. P., Putaux, J. L., Chanzy, H. (2000) Growth and kinetics of in vitro poly([R]-(–)–3-hydroxybutyrate) granules interpreted as particulate polymerization with coalescence, Macromol. Rapid Commun. 21, 77–84.
- Peoples, O. P., Sinskey, A. J. (1989) Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16 Identification and characterization of the PHB polymerase gene (phbC), J. Biol. Chem. 264, 15298–15303.
- Peoples, O. P., Gerngross, T. U., Sinskey, A. J. (1996) Overproduction and purification of soluble PHA synthase, US Patent 5480794.
- Qi, Q., Steinbüchel, A., Rehm, B. H. A. (2000) In vitro synthesis of poly(3-hydroxydecanoate): purification and enzymatic characterization of type II poly(hydroxyalkanoate synthase of PhaC1 and PhaC2 from Pseudomonas aeruginosa, Appl. Microbiol. Biotechnol. 54, 37–43.
- Rehm, B. H. A., Steinbüchel, A. (1999) Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis, Int. J. Biol. Macromol. 25, 3–19.
- Ren, J., de Roo, G., Kessler, B., Witholt, B. (2000) Recovery of active medium-chain-length poly-3-hydroxyalkanoate polymerase from inactive inclusion bodies using ion-exchange resin, Biochem. J. 349, 599–604.
- Rothermich, M. M., Guerrero, R., Lenz, R. W., Goodwin, S. (2000) Poly(hydroxyalkanoate) characterization and diel fluctuation in photosynthetic microbial mats, Appl. Env. Microbiol. 66, 4279–4291.
- Song, J. J., Zhang, S., Lenz, R. W., Goodwin, S. (2000) In vitro polymerization and copolymerization of 3-hydroxypropionyl-CoA with the PHB synthase from Ralstonia eutropha, Biomacromolecules 1, 433–439.
- Su, L., Lenz, R. W., Takagi, Y., Zhang, S., Goodwin, S., Zhong, L., Martin, D. P. (2000) Enzymatic polymerization of [R]-3-hydroxyalkanoates by a bacterial polymerase, Macromolecules 33, 229–231.
- Tomita, K., Saito, T., Fukui, T. (1983) Bacterial metabolism of poly-β-hydroxybutyrate, in: Biochemistry of Metabolic Processes ( D. F. Lennon, F. W. Stratman, R. N. Zahlten, Eds), New York: Elsevier, 353–356.
- Valentin, H. E., Broyles, D. L., Casagrande, L. A., Colburn, S. M., Creely, W. L., DeLaquil, P. A., Felton, H. M., Gonzalez, K. A., Houmiel, K. L., Lutke, K., Mahadeo, D. A., Mitsky, T. A., Padgette, S. R., Reiser, S. E., Slater, S., Stark, D. M., Stock, R. T., Stone, D. A., Taylor, N. B., Thorne, G. M., Tran, M., Gruys, K. J. (1999) PHA production, from bacteria to plants, Int. J. Biol. Macromol. 25, 303–306.
- Williams, M. D., Fieno, A. M., Grant, R. A., Sherman, D. H. (1996) Expression and analysis of a bacterial poly(hydroxyalkanoate) synthase in insect cells using a baculovirus system, Prot. Expr. Purific. 7, 203–211.
- Wodzinska, J., Snell, K. D., Rhomberg, A., Sinskey, A. J., Biemann, K., Stubbe, J. (1996) Polyhydroxybutyrate synthase: evidence for covalent catalysis, J. Am. Chem. Soc. 118, 6319–6320.
- Zhang, S., Yasuo, T., Lenz, R. W., Goodwin, S. (2000) Kinetic and mechanistic characterization of the poly(hydroxybutyrate) synthase from Ralstonia eutropha, Biomacromolecules 1, 244–251.
- Zhang, S., Kamachi, M., Takagi, Y., Lenz, R. W., Goodwin, S. (2001a) Comparative study of the relationship between monomer structure and reactivity for two polyhydroxyalkanoate synthases, Appl. Microbiol. Biotechnol., in press.
- Zhang, S., Kolvek, S., Lenz, R. W., Goodwin, S. (2001b) Dimerization is required for the polymerization activity of the polyhydroxybutyrate synthase from Ralstonia eutropha and a single cysteine is not sufficient for catalysis to J. Bacteriol., submitted.
- Zhang, S., Kolvek, S., Lenz, R. W., Goodwin, S. (2001c) Poly(hydroxyalkanoic acid) biosynthesis in Ectothiorhodospira shaposhnikovii: characterization and catalytic properties of a type III PHA synthase, J. Bacteriol., submitted.
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