In vitro Biosynthesis of Polyhydroxyalkanoates

Part 3a. Polyesters
Dr. Shiming Zhang

Dr. Shiming Zhang

University of Massachusetts, Department of Microbiology, Amherst, MA, USA, 01003

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Prof. Dr. Robert W. Lenz

Prof. Dr. Robert W. Lenz

University of Massachusetts, Polymer Science and Engineering Department, Amherst, MA, USA, 01003

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Prof. Dr. Steve Goodwin

Prof. Dr. Steve Goodwin

University of Massachusetts, Department of Microbiology, Amherst, MA, USA, 01003

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First published: 15 January 2005
Citations: 1

Abstract

  • Introduction
  • Historical Outline
    • Characterization of PHA Synthase Activity
    • Isolation and Activity of Soluble PHA Synthase
  • Properties of PHA Synthases
    • Lag Phase in Polymerization Reaction
    • Elimination of Lag Phase
    • Substrate Specificity
  • Mechanism of Polymerization Reaction
  • In vitro Polymerization of HACoA Monomers
    • Control of Polymer Properties
      • Molecular Weight
      • Repeating Unit Distribution
      • Molecular Weight Polydispersity
      • Stabilizing of PHA Synthase Activity
    • New Types of PHAs
    • Diversity of PHA Synthases
  • CoA Recycling Systems
  • Patents
  • Outlook and Perspectives

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