Polyhydroxyalkanoate (PHA) Synthases: The Key Enzymes of PHA Synthesis

Part 3a. Polyesters
Dr. Bernd H. A. Rehm

Dr. Bernd H. A. Rehm

Westfälische Wilhelms-Universität Münster, Institut für Mikrobiologie, Corrensstraße 3, Münster, Germany, D-48149

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Prof. Dr. Alexander Steinbüchel

Prof. Dr. Alexander Steinbüchel

Westfälische Wilhelms-Universität Münster, Institut für Mikrobiologie, Corrensstraße 3, Münster, Germany, D-48149

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First published: 15 January 2005
Citations: 15

Abstract

  • Introduction
  • Terminology
  • Cloning Strategies for PHA Synthase Genes
  • Organization of PHA Synthase Genes
  • Primary Structures of PHA Synthases
  • Secondary and Quaternary Structures of PHA Synthases
    • In vivo versus in vitro Substrate Specificity of PHA Synthases
  • Development of a Topological Model for PHA Synthases and Analysis of Site-specific Mutants of the PHA Synthases
  • The Proposed Catalytic Mechanism of PHA Synthases
  • Factors Determining the Molecular Weight and Composition of PHAs
  • PHA Granules
  • In vitro Synthesis of PHA
  • Diversion of Intermediates from Central Pathways to PHA Biosynthesis
  • Conclusions and Outlook
  • Acknowledgments

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