Accuracy of 1H and 31P MRS analyses of lactate in skeletal muscle
Corresponding Author
Alex C. Hsu
Biomedical Magnetic Resonance Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
College of Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois
Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, 524 Burrill Hall, 407 S. Goodwin Ave., Urbana, IL 61801===Search for more papers by this authorM. Joan Dawson
Biomedical Magnetic Resonance Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, Illinois
College of Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois
Search for more papers by this authorCorresponding Author
Alex C. Hsu
Biomedical Magnetic Resonance Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
College of Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois
Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, 524 Burrill Hall, 407 S. Goodwin Ave., Urbana, IL 61801===Search for more papers by this authorM. Joan Dawson
Biomedical Magnetic Resonance Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, Illinois
College of Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois
Search for more papers by this authorAbstract
As the end product of anaerobic metabolism and a source of H+, lactic acid is important in metabolism and pH regulation. Several methods have been introduced to calculate changes in the lactate anion (Lac–) concentration in exercising skeletal muscle from information derived from the 31P spectrum. Alternatively, Lac–may be observed directly with 1H MRS. Both 1H and 31P spectroscopy have potential problems, which could prevent accurate determination of [Lac–]. It is demonstrated that quantitatively accurate 1H MRS measurements of changes in [Lac–] due to exercise are possible in isolated muscle. In general, calculation by 31P MRS overestimates Lac–production. An analysis is presented of possible sources of errors in the 1H and 31P MRS methods. Magn Reson Med 44:418–426, 2000. © 2000 Wiley-Liss, Inc.
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