Up-regulated expression of decay-accelerating factor (CD55) confers increased complement resistance to sprouting neural cells
Ke-zhou Zhang
Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
Search for more papers by this authorSami Junnikkala
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
Search for more papers by this authorMark G. Erlander
R. W. Johnson Pharmaceutical Research Institute, San Diego, USA
Search for more papers by this authorHongqing Guo
R. W. Johnson Pharmaceutical Research Institute, San Diego, USA
Search for more papers by this authorJohan A. Westberg
Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
Search for more papers by this authorSeppo Meri
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
Search for more papers by this authorLeif C. Andersson
Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
Department of Pathology, Karolinska Institute, Stockholm, Sweden
Search for more papers by this authorKe-zhou Zhang
Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
Search for more papers by this authorSami Junnikkala
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
Search for more papers by this authorMark G. Erlander
R. W. Johnson Pharmaceutical Research Institute, San Diego, USA
Search for more papers by this authorHongqing Guo
R. W. Johnson Pharmaceutical Research Institute, San Diego, USA
Search for more papers by this authorJohan A. Westberg
Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
Search for more papers by this authorSeppo Meri
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
Search for more papers by this authorLeif C. Andersson
Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
Department of Pathology, Karolinska Institute, Stockholm, Sweden
Search for more papers by this authorAbstract
We studied gene expression in relation to induced neural differentiation in a human neural crest-derived cell line, Paju. Messenger RNA isolated before and after treatment with phorbol 12-myristate 13-acetate was analyzed by differential display reverse transcription PCR. A strongly up-regulated expression of decay-accelerating factor (DAF, CD55) was found to parallel the induced neural sprouting while the expression of two other complement regulatory proteins (CD59/protectin, CD46/membrane cofactor protein) remained unaltered during neural differentiation. The increased membrane expression of DAF, which was also seen on neural processes and growth cones, conferred elevated resistance to complement-mediated lysis. Our findings suggest that in sprouting neurons DAF expression is up-regulated to provide additional complement resistance to pathfinding axons/dendrites invading new environment. It is also suggested that membrane expression of DAF may constitute a marker of growing and regenerating neurons.
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