Two flow cytometric techniques were used to measure rapid transient changes in [Ca²⁺]_i in the neuronal cell line NH15-CA2. Using on-line injection, the cell suspension and stimulating solution are mixed and delivered to the detection point by a rapid increase in sample pressure. In NH15-CA2, injection of medium alone resulted in [Ca²⁺]_i increase. Using the fixed-time method, where cells are maintained at constant pressure, no [Ca²⁺]_i increase was observed with medium alone. These results show that a rapid pressure increase alone alters the [Ca²⁺]_i in NH15-CA2 cells. Both methods showed similar kinetics of [Ca²⁺]_i in response to bradykinin but the fixed-time method was found to be better for determination of the percentage of responsive cells. © 1996 Wiley-Liss, Inc.

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Volume23, Issue1

1 January 1996

Pages 82-89

Improved kinetic analysis of cytosolic free calcium in pressure-sensitive neuronal cells by fixed-time flow cytometry

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Improved kinetic analysis of cytosolic free calcium in pressure-sensitive neuronal cells by fixed-time flow cytometry

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