Expression of the ALK protein by anaplastic large-cell lymphomas correlates with high proliferative activity
Lorenzo Leoncini
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorStefano Lazzi
Institute of Pathologic Anatomy and Histology, University of Siena, Siena, Italy
Search for more papers by this authorDonatella Scano
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorAntonina Mura
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorAngela Onida
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorGiovannino Massarelli
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorCorresponding Author
Piero Tosi
Institute of Pathologic Anatomy and Histology, University of Siena, Siena, Italy
Institute of Pathologic Anatomy and Histology, University of Siena, Via delle Scotte 6, I-53100 Siena, Italy. Fax: +(39)0577 233 235Search for more papers by this authorPaolo Barbini
Institute of Thoracic and Cardiovascular Surgery and Biomedical Technology, University of Siena, Siena, Italy
Search for more papers by this authorGabriele Cevenini
Institute of Thoracic and Cardiovascular Surgery and Biomedical Technology, University of Siena, Siena, Italy
Search for more papers by this authorMaria Rita Massai
Institute of Thoracic and Cardiovascular Surgery and Biomedical Technology, University of Siena, Siena, Italy
Search for more papers by this authorStefano Pileri
Institute of Hematology “L. & A. Seràgnoli”, Hemolymphopathology Unit, University of Bologna, Bologna, Italy
Search for more papers by this authorBrunangelo Falini
Institute of Hematology, University of Perugia, Perugia, Italy
Search for more papers by this authorAntonio Giordano
Thomas Jefferson Medical College, Philadelphia, Pennsylvania, USA
Search for more papers by this authorRainer Kraft
Institute of Pathology, University of Berne, Berne, Switzerland
Search for more papers by this authorJean A. Laissue
Institute of Pathology, University of Berne, Berne, Switzerland
Search for more papers by this authorHans Cottier
Institute of Pathology, University of Berne, Berne, Switzerland
Search for more papers by this authorLorenzo Leoncini
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorStefano Lazzi
Institute of Pathologic Anatomy and Histology, University of Siena, Siena, Italy
Search for more papers by this authorDonatella Scano
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorAntonina Mura
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorAngela Onida
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorGiovannino Massarelli
Institute of Pathology, University of Sassari, Sassari, Italy
Search for more papers by this authorCorresponding Author
Piero Tosi
Institute of Pathologic Anatomy and Histology, University of Siena, Siena, Italy
Institute of Pathologic Anatomy and Histology, University of Siena, Via delle Scotte 6, I-53100 Siena, Italy. Fax: +(39)0577 233 235Search for more papers by this authorPaolo Barbini
Institute of Thoracic and Cardiovascular Surgery and Biomedical Technology, University of Siena, Siena, Italy
Search for more papers by this authorGabriele Cevenini
Institute of Thoracic and Cardiovascular Surgery and Biomedical Technology, University of Siena, Siena, Italy
Search for more papers by this authorMaria Rita Massai
Institute of Thoracic and Cardiovascular Surgery and Biomedical Technology, University of Siena, Siena, Italy
Search for more papers by this authorStefano Pileri
Institute of Hematology “L. & A. Seràgnoli”, Hemolymphopathology Unit, University of Bologna, Bologna, Italy
Search for more papers by this authorBrunangelo Falini
Institute of Hematology, University of Perugia, Perugia, Italy
Search for more papers by this authorAntonio Giordano
Thomas Jefferson Medical College, Philadelphia, Pennsylvania, USA
Search for more papers by this authorRainer Kraft
Institute of Pathology, University of Berne, Berne, Switzerland
Search for more papers by this authorJean A. Laissue
Institute of Pathology, University of Berne, Berne, Switzerland
Search for more papers by this authorHans Cottier
Institute of Pathology, University of Berne, Berne, Switzerland
Search for more papers by this authorAbstract
A variable fraction of anaplastic large-cell lymphomas (ALCLs) exhibits a t(2;5)(p23;q35) translocation that results in expression of the chimeric hyperphosphorylated protein NPM-ALK (p80). Tumor cells expressing NPM-ALK exhibit markedly enhanced proliferative activity, but comparative cellular kinetic studies on ALK+ (ALK lymphomas) and ALK– lymphomas are lacking. The present study showed that ALK+ lymphomas, detected with the monoclonal antibody ALKc (n = 17), had significantly higher average values for the proliferation-associated parameters mitotic index, ana/telophase index, growth index (x × mitotic index – apoptotic index, assuming x = 3), percentages of Ki-67+ cells and fraction of cells expressing cyclin A or B or the cell cycle–regulatory protein p34cdc2 than did ALK– ALCLs (n = 15). Whether this intense proliferative activity contributes to the good response to chemotherapy and favorable outcome of ALK+ ALCLs remains to be assessed in a larger series of patients. Our findings support the notion that ALK+ and ALK– ALCLs are 2 distinct disease entities. Int. J. Cancer 86:777–781, 2000. © 2000 Wiley-Liss, Inc.
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