Experimental Cancer

Stimulation of glioma-cell migration by laminin and inhibition by anti-α3 and anti-β1 integrin antibodies

Corresponding Author

Berit B. Tysnes

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

Department of Anatomy and Cell Biology, University of Bergen, Aarstadvollen 19, N-5009 Bergen, Norway. Fax: 47 55206360Search for more papers by this author

Lone F. Larsen,

Lone F. Larsen

Department of Neurosurgery, Henry Ford Hospital, Detroit, MI USA

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Gro O. Ness,

Gro O. Ness

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

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Rupavathana Mahesparan,

Rupavathana Mahesparan

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

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Klaus Edvardsen,

Klaus Edvardsen

Department of Neurosurgery, Henry Ford Hospital, Detroit, MI USA

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Inmaculada Garcia-Cabrera,

Inmaculada Garcia-Cabrera

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

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Rolf Bjerkvig,

Rolf Bjerkvig

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

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Berit B. Tysnes,

Corresponding Author

Berit B. Tysnes

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

Department of Anatomy and Cell Biology, University of Bergen, Aarstadvollen 19, N-5009 Bergen, Norway. Fax: 47 55206360Search for more papers by this author

Lone F. Larsen,

Lone F. Larsen

Department of Neurosurgery, Henry Ford Hospital, Detroit, MI USA

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Gro O. Ness,

Gro O. Ness

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

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Rupavathana Mahesparan,

Rupavathana Mahesparan

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

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Klaus Edvardsen,

Klaus Edvardsen

Department of Neurosurgery, Henry Ford Hospital, Detroit, MI USA

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Inmaculada Garcia-Cabrera,

Inmaculada Garcia-Cabrera

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

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Rolf Bjerkvig,

Rolf Bjerkvig

Departments of Anatomy and Cell Biology and Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

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First published: 17 September 1996

https://doi.org/10.1002/(SICI)1097-0215(19960917)67:6<777::AID-IJC5>3.0.CO;2-O

Citations: 68

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Abstract

An induction of laminin in the confrontation zone between tumor cells and normal brain tissue has been observed in our model systems in vivo and in vitro. In order to study the effects of ECM components on glioma-cell migration and invasion, we have used 2 lacZ-transfected glioma cell lines, ANI/lacZ and U-251/lacZ. Cell migration from multicellular spheroids was studied using different types of media: DMEM with 10% serum, Ultra Culture medium, and filtrated DMEM with serum in which the protein fraction > 100 kDa had been removed by ultrafiltration. Laminin, fibronectin and collagen type-IV were individually added to the different media, and cell migration from the spheroids was studied. The results show that cell migration in both cell lines, was stimulated by laminin and fibronectin. Collagen type-IV stimulated only cell migration of U-251/lacZ cells. Scanning electron microscopy revealed an extensive change in cell shape as a result of laminin stimulation. Flow-cytometric studies showed that both ANI/lacZ and U-251/lacZ strongly express the α3β1 integrin receptor, which can bind to several ECM components (laminin, fibronectin, collagen). Immunofluorescence microscopy demonstrated that the same integrin sub-units were expressed in multicellular spheroids. When monoclonal antibodies to α3 and β1 were added to the laminin-stimulated cultures, cell migration was significantly reduced. This indicates that the α3β1 integrin receptor plays an important role during glioma-cell migration. © 1996 Wiley-Liss, Inc.

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Volume67, Issue6

17 September 1996

Pages 777-784

Stimulation of glioma-cell migration by laminin and inhibition by anti-α3 and anti-β1 integrin antibodies

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Stimulation of glioma-cell migration by laminin and inhibition by anti-α3 and anti-β1 integrin antibodies

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