Detection of human herpes virus 8 DNA and sequence polymorphism in classical, epidemic, and iatrogenic Kaposi's sarcoma in South Africa
Corresponding Author
Susan Engelbrecht
Department of Medical Virology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Department of Medical Virology, P.O. Box 19063, 7505 Tygerberg, South Africa===Search for more papers by this authorFlorette K. Treurnicht
Department of Medical Virology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorJohann W. Schneider
Department of Anatomical Pathology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorH. Francois Jordaan
Department of Dermatology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorJan G. Steytler
Department of Medical Virology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorPeter A. B. Wranz
Department of Anatomical Pathology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorEstrelita J. van Rensburg
Department of Medical Virology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorCorresponding Author
Susan Engelbrecht
Department of Medical Virology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Department of Medical Virology, P.O. Box 19063, 7505 Tygerberg, South Africa===Search for more papers by this authorFlorette K. Treurnicht
Department of Medical Virology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorJohann W. Schneider
Department of Anatomical Pathology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorH. Francois Jordaan
Department of Dermatology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorJan G. Steytler
Department of Medical Virology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorPeter A. B. Wranz
Department of Anatomical Pathology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorEstrelita J. van Rensburg
Department of Medical Virology University of Stellenbosch and Tygerberg Hospital, Tygerberg, South Africa
Search for more papers by this authorAbstract
The aetiology and detection of human herpes virus type 8 (HHV-8) DNA sequences in Kaposi's sarcoma (KS) is a matter of intense investigation. We report on the detection of HHV-8 DNA and sequence polymorphism in different clinicopathological subtypes of cutaneous KS samples from South Africa. The diagnosis was confirmed by histological examination in all cases. Six patients had classic KS (CKS), 3 epidemic KS (EKS), and 3 iatrogenic KS (IKS). A nested polymerase chain reaction (PCR) assay was used to detect HHV-8 DNA in cell lysates, prepared from formalin fixed, paraffin embedded sections. We investigated polymorphism in the HHV-8 DNA using single-stranded conformational polymorphism (SSCP) analysis on the PCR products, followed by direct sequencing. HHV-8 DNA was detected in all the patients with KS, irrespective of the clinicopathological subtype. Direct sequencing was performed on 5 selected cases and showed single base pair substitutions in all. The spectrum of mutations was similar to those described previously. No correlation was found between the different types of KS and sequence variation. The results support the hypothesis that HHV-8 is strongly associated with different clinicopathological subtypes of KS and confirm the occurrence of HHV-8 in patients with CKS, EKS, and IKS in South Africa. J. Med. Virol. 52:168–172, 1997. © 1997 Wiley-Liss, Inc.
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