Volume 115, Issue 6 pp. 838-840
Original Articles

Flow-Cytometric Confirmation of Aneuploidy in Sperm from Triploid Rainbow Trout

Tillmann J. Benfey

Tillmann J. Benfey

Department of Fisheries and Oceans, Fisheries Research Branch, West Vancouver Laboratory, 4160 Marine Drive, West Vancouver, British Columbia, V7V 1N6 Canada

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Igor I. Solar

Igor I. Solar

Department of Fisheries and Oceans, Fisheries Research Branch, West Vancouver Laboratory, 4160 Marine Drive, West Vancouver, British Columbia, V7V 1N6 Canada

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Gary De Jong

Gary De Jong

Cancer Control Agency of British Columbia, 600 West 10th Avenue, Vancouver, British Columbia, V5Z 4E6 Canada

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Edward M. Donaldson

Edward M. Donaldson

Department of Fisheries and Oceans, Fisheries Research Branch, West Vancouver Laboratory, 4160 Marine Drive, West Vancouver, British Columbia, V7V 1N6 Canada

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Abstract

Triploid rainbow trout Salmo gairdneri produce spermatozoa with DNA contents intermediate between haploid and diploid values. This confirms that these spermatozoa are aneuploid and explains why progeny produced from the fertilization of haploid eggs with such sperm are not viable. Triploid males have significantly lower spermatocrit and smaller testes than diploids, but still develop normal secondary sexual characteristics and exhibit postspawning mortality.

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