Corrigendum to “Clinical Relevance and Tumor Growth Suppression of Mitochondrial ROS Regulators Along NADH:Ubiquinone Oxidoreductase Subunit B3 in Thyroid Cancer”
First published: 26 July 2025
J. Zhu, X. Zheng, D. Lu, Y. Zheng, J. Liu, “Clinical Relevance and Tumor Growth Suppression of Mitochondrial ROS Regulators Along NADH:Ubiquinone Oxidoreductase Subunit B3 in Thyroid Cancer,” Oxidative Medicine and Cellular Longevity 2022 (2022): 8038857, https://doi.org/10.1155/2022/8038857.
During the production process for this article, errors were introduced to Figures 4 and 5, and their accompanying legends:
Figure 4 (A)
The role of NDUFB3 overexpression in mitochondrial respiration and mitoROS generation. (A) Volcano plots were constructed using fold-change values and adjusted p (NDUFB3low versus NDUFB3high). The red point in the plot represents the overexpressed mRNAs, and the blue point indicates the downregulated mRNAs with statistical significance. The plot of NDUFB3 is shown on the top, as indicated. (B) Volcano plots were constructed using enrichment score and FDR. The number of genes enriched in each KEGG pathway is shown in the top-left corner. In the enrichment result, p < 0.05 or FDR <0.05 is considered to be enriched to a meaningful pathway. (C) GSEA histogram for the gene set “OXPHOS.” (D) Real-time OCR tracing of BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 3). (E) Total cellular ATP concentration in BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 5). Statistical differences were determined by a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (F) Complex I activity was determined in mitochondria isolated from BCPAP cells or C643 cells overexpressing NDFUB3 or empty vector control (n = 5). Statistical differences were determined using a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (G) Representative flow cytometry analysis of NDUFB3 overexpression or its empty vector control showing respective mitoROS levels in BCPAP or c643 cells.
Figure 4 (B)
The role of NDUFB3 overexpression in mitochondrial respiration and mitoROS generation. (A) Volcano plots were constructed using fold-change values and adjusted p (NDUFB3low versus NDUFB3high). The red point in the plot represents the overexpressed mRNAs, and the blue point indicates the downregulated mRNAs with statistical significance. The plot of NDUFB3 is shown on the top, as indicated. (B) Volcano plots were constructed using enrichment score and FDR. The number of genes enriched in each KEGG pathway is shown in the top-left corner. In the enrichment result, p < 0.05 or FDR <0.05 is considered to be enriched to a meaningful pathway. (C) GSEA histogram for the gene set “OXPHOS.” (D) Real-time OCR tracing of BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 3). (E) Total cellular ATP concentration in BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 5). Statistical differences were determined by a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (F) Complex I activity was determined in mitochondria isolated from BCPAP cells or C643 cells overexpressing NDFUB3 or empty vector control (n = 5). Statistical differences were determined using a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (G) Representative flow cytometry analysis of NDUFB3 overexpression or its empty vector control showing respective mitoROS levels in BCPAP or c643 cells.
Figure 4 (C)
The role of NDUFB3 overexpression in mitochondrial respiration and mitoROS generation. (A) Volcano plots were constructed using fold-change values and adjusted p (NDUFB3low versus NDUFB3high). The red point in the plot represents the overexpressed mRNAs, and the blue point indicates the downregulated mRNAs with statistical significance. The plot of NDUFB3 is shown on the top, as indicated. (B) Volcano plots were constructed using enrichment score and FDR. The number of genes enriched in each KEGG pathway is shown in the top-left corner. In the enrichment result, p < 0.05 or FDR <0.05 is considered to be enriched to a meaningful pathway. (C) GSEA histogram for the gene set “OXPHOS.” (D) Real-time OCR tracing of BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 3). (E) Total cellular ATP concentration in BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 5). Statistical differences were determined by a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (F) Complex I activity was determined in mitochondria isolated from BCPAP cells or C643 cells overexpressing NDFUB3 or empty vector control (n = 5). Statistical differences were determined using a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (G) Representative flow cytometry analysis of NDUFB3 overexpression or its empty vector control showing respective mitoROS levels in BCPAP or c643 cells.
Figure 4 (D)
The role of NDUFB3 overexpression in mitochondrial respiration and mitoROS generation. (A) Volcano plots were constructed using fold-change values and adjusted p (NDUFB3low versus NDUFB3high). The red point in the plot represents the overexpressed mRNAs, and the blue point indicates the downregulated mRNAs with statistical significance. The plot of NDUFB3 is shown on the top, as indicated. (B) Volcano plots were constructed using enrichment score and FDR. The number of genes enriched in each KEGG pathway is shown in the top-left corner. In the enrichment result, p < 0.05 or FDR <0.05 is considered to be enriched to a meaningful pathway. (C) GSEA histogram for the gene set “OXPHOS.” (D) Real-time OCR tracing of BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 3). (E) Total cellular ATP concentration in BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 5). Statistical differences were determined by a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (F) Complex I activity was determined in mitochondria isolated from BCPAP cells or C643 cells overexpressing NDFUB3 or empty vector control (n = 5). Statistical differences were determined using a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (G) Representative flow cytometry analysis of NDUFB3 overexpression or its empty vector control showing respective mitoROS levels in BCPAP or c643 cells.
Figure 4 (E)
The role of NDUFB3 overexpression in mitochondrial respiration and mitoROS generation. (A) Volcano plots were constructed using fold-change values and adjusted p (NDUFB3low versus NDUFB3high). The red point in the plot represents the overexpressed mRNAs, and the blue point indicates the downregulated mRNAs with statistical significance. The plot of NDUFB3 is shown on the top, as indicated. (B) Volcano plots were constructed using enrichment score and FDR. The number of genes enriched in each KEGG pathway is shown in the top-left corner. In the enrichment result, p < 0.05 or FDR <0.05 is considered to be enriched to a meaningful pathway. (C) GSEA histogram for the gene set “OXPHOS.” (D) Real-time OCR tracing of BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 3). (E) Total cellular ATP concentration in BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 5). Statistical differences were determined by a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (F) Complex I activity was determined in mitochondria isolated from BCPAP cells or C643 cells overexpressing NDFUB3 or empty vector control (n = 5). Statistical differences were determined using a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (G) Representative flow cytometry analysis of NDUFB3 overexpression or its empty vector control showing respective mitoROS levels in BCPAP or c643 cells.
Figure 4 (F)
The role of NDUFB3 overexpression in mitochondrial respiration and mitoROS generation. (A) Volcano plots were constructed using fold-change values and adjusted p (NDUFB3low versus NDUFB3high). The red point in the plot represents the overexpressed mRNAs, and the blue point indicates the downregulated mRNAs with statistical significance. The plot of NDUFB3 is shown on the top, as indicated. (B) Volcano plots were constructed using enrichment score and FDR. The number of genes enriched in each KEGG pathway is shown in the top-left corner. In the enrichment result, p < 0.05 or FDR <0.05 is considered to be enriched to a meaningful pathway. (C) GSEA histogram for the gene set “OXPHOS.” (D) Real-time OCR tracing of BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 3). (E) Total cellular ATP concentration in BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 5). Statistical differences were determined by a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (F) Complex I activity was determined in mitochondria isolated from BCPAP cells or C643 cells overexpressing NDFUB3 or empty vector control (n = 5). Statistical differences were determined using a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (G) Representative flow cytometry analysis of NDUFB3 overexpression or its empty vector control showing respective mitoROS levels in BCPAP or c643 cells.
Figure 4 (G)
The role of NDUFB3 overexpression in mitochondrial respiration and mitoROS generation. (A) Volcano plots were constructed using fold-change values and adjusted p (NDUFB3low versus NDUFB3high). The red point in the plot represents the overexpressed mRNAs, and the blue point indicates the downregulated mRNAs with statistical significance. The plot of NDUFB3 is shown on the top, as indicated. (B) Volcano plots were constructed using enrichment score and FDR. The number of genes enriched in each KEGG pathway is shown in the top-left corner. In the enrichment result, p < 0.05 or FDR <0.05 is considered to be enriched to a meaningful pathway. (C) GSEA histogram for the gene set “OXPHOS.” (D) Real-time OCR tracing of BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 3). (E) Total cellular ATP concentration in BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (n = 5). Statistical differences were determined by a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (F) Complex I activity was determined in mitochondria isolated from BCPAP cells or C643 cells overexpressing NDFUB3 or empty vector control (n = 5). Statistical differences were determined using a two-tailed unpaired Mann–Whitney t-test. ∗p < 0.05, ∗∗p < 0.01. (G) Representative flow cytometry analysis of NDUFB3 overexpression or its empty vector control showing respective mitoROS levels in BCPAP or c643 cells.
Figure 5 (A)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (B)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (C)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (D)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (E)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (F)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (G)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (H)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (I)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (J)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (K)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
Figure 5 (L)
Overexpression of NDUFB3 or sideroxylin suppresses thyroid tumor growth in vivo. (A) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably overexpressing NDUFB3 or its empty control vector in dorsal tissues. (B) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (C) Survival curves of tumor-bearing mice overexpressing NDUFB3 or empty vector controls. Analysis was statistically evaluated using the log-rank test. (D) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vector versus NDUFB3). (E) A sketch map showing mice bearing thyroid tumors derived from BCPAP or C643 cells stably expressing shRNA against NDUFB3 or its empty control vector in dorsal tissues. (F) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01. (G) The survival curves of tumor-bearing mice with NDUFB3 knockdown or its empty vector controls. Analysis was statistically evaluated using the log-rank test. (H) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (shNDUFB3 versus shCtrl). (I) A sketch map showing that mice bearing thyroid tumors were treated with sideroxylin or vehicle after BCPAP cells or C643 cells were inoculated into the dorsal tissues of mice. (J) Tumor volumes over the indicated times were analyzed. Mice were euthanized as their tumor volumes reached ~3000 mm3. Each line represents a mouse, and each plot on the line represents a different time point. p-Values represent two-way ANOVA and post hoc Tuckey’s tests for tumor growth. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (K) Survival curves of tumor-bearing mice treated with sideroxylin or vehicle. Analysis was statistically evaluated using the log-rank test. (L) Representative flow cytometry analysis of single-cell suspensions from resected tumor masses (vehicle versus sideroxylin).
The correct Figures 4 and 5, along with their legends, are shown below:
We apologize for these errors.
