This chapter reviews many commonly used organisms, experimental approaches, and techniques used for the growth and study of biofilms. Here, the authors focus on commonly used approaches with a greater emphasis on currently used techniques. They have summarized several diverse techniques for growing and evaluating biofilms below. These include examples of biofilm techniques for exploring naturally occurring biofilms, medically important biofilms, industrial applications, laboratory biofilm techniques, imaging and spectroscopy techniques, and broad-based genetic techniques. Overall, the authors concentrate on several techniques that are presently used in current biofilm research. Included in this discussion are biofilm growth strategies, a brief mention of genetic strategies, imaging techniques, and data analysis. The authors hope that this discussion will serve as an informative reference for the biofilm research community. In the field of dental microbiology, the constant depth fermenter is a device in which oral microorganisms are cultured on hydroxyapatite disks that are coated with saliva. Such standardized methods will permit a meaningful and rational comparison of data among individual laboratories. Finally, microbiologists now realize the many diverse environments and situations in which biofilms occur. As a result, there will be a continuing need for innovation in the experimental designs used to study biofilms.