Secreted mucins and airway bacterial colonization in non-CF bronchiectasis
Corresponding Author
Oriol Sibila
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Correspondence: Oriol Sibila, Respiratory Department, Hospital de la Santa Creu i Sant Pau. Sant Antoni M. Claret Street 167, Barcelona 08025, Spain. Email: [email protected]Search for more papers by this authorGuillermo Suarez-Cuartin
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorAna Rodrigo-Troyano
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorThomas C. Fardon
Tayside Respiratory Research Group, University of Dundee, Dundee, UK
Search for more papers by this authorSimon Finch
Tayside Respiratory Research Group, University of Dundee, Dundee, UK
Search for more papers by this authorEder Freddy Mateus
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Laboratory of Experimental Immunology, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorLaia Garcia-Bellmunt
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorDiego Castillo
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorSilvia Vidal
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Laboratory of Experimental Immunology, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorFerran Sanchez-Reus
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorMarcos I. Restrepo
Critical Care Department, South Texas Veterans Health Care System
Medicine Division, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
Search for more papers by this authorJames D. Chalmers
Tayside Respiratory Research Group, University of Dundee, Dundee, UK
Search for more papers by this authorCorresponding Author
Oriol Sibila
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Correspondence: Oriol Sibila, Respiratory Department, Hospital de la Santa Creu i Sant Pau. Sant Antoni M. Claret Street 167, Barcelona 08025, Spain. Email: [email protected]Search for more papers by this authorGuillermo Suarez-Cuartin
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorAna Rodrigo-Troyano
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorThomas C. Fardon
Tayside Respiratory Research Group, University of Dundee, Dundee, UK
Search for more papers by this authorSimon Finch
Tayside Respiratory Research Group, University of Dundee, Dundee, UK
Search for more papers by this authorEder Freddy Mateus
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Laboratory of Experimental Immunology, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorLaia Garcia-Bellmunt
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorDiego Castillo
Respiratory Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorSilvia Vidal
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Laboratory of Experimental Immunology, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorFerran Sanchez-Reus
Biomedical Research Institute Sant Pau (IIB Sant Pau), Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain
Search for more papers by this authorMarcos I. Restrepo
Critical Care Department, South Texas Veterans Health Care System
Medicine Division, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
Search for more papers by this authorJames D. Chalmers
Tayside Respiratory Research Group, University of Dundee, Dundee, UK
Search for more papers by this authorAbstract
Background and objective
Secreted mucins play a key role in antibacterial defence in the airway, but have not previously been characterized in non-cystic fibrosis (CF) bronchiectasis patients. We aim to investigate the relationship between secreted mucins levels and the presence of bacterial colonization due to potentially pathogenic microorganisms (PPM) in the airways of stable bronchiectasis patients.
Methods
Clinically stable bronchiectasis patients were studied prospectively at two centres. Patients with other pulmonary conditions were excluded. Spontaneous sputum was subject to bacterial culture, and secreted mucins (MUC2, MUC5AC and MUC5B) were measured in sputum supernatants by ELISA.
Results
A total of 50 patients were included. PPM were identified from sputum samples in 30 (60%), with Pseudomonas aeruginosa (n = 10) and Haemophilus influenzae (n = 10) as the most common PPM. There were no baseline differences among airway colonized and non-colonized patients. Patients with airways colonized by PPM presented higher levels of airway MUC2. No differences in MUC5AC levels were found among groups, whereas MUC5B levels were undetectable. Patients with P. aeruginosa colonization expressed the highest levels of MUC2. High levels of MUC2 and MUC5AC are also correlated with disease severity using the Bronchiectasis Severity Index.
Conclusions
Airway MUC2 levels were higher in bronchiectasis patients colonized with PPM compared with those without airway colonization, especially in patients with P. aeruginosa. These findings suggest that airway-secreted mucins levels may play a role in the pathogenesis of airway infection in non-CF bronchiectasis.
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