Volume 19, Issue 1 pp. 122-131

ORIGINAL ARTICLE

Der p2 recombinant bacille Calmette-Guerin priming of bone marrow-derived dendritic cells suppresses Der p2-induced T helper17 function in a mouse model of asthma

Shuo-Yao Qu,

Shuo-Yao Qu

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

These authors contributed equally to this study.Search for more papers by this author

Hai-Feng Ou-yang,

Hai-Feng Ou-yang

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

These authors contributed equally to this study.Search for more papers by this author

Ya-Long He,

Ya-Long He

Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China

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Zhi-Kui Li,

Zhi-Kui Li

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

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Jie-Ran Shi,

Jie-Ran Shi

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

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Li-Qiang Song,

Li-Qiang Song

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

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Chang-Gui Wu,

Corresponding Author

Chang-Gui Wu

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

Correspondence: Chang-Gui Wu, Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. Email: [email protected]Search for more papers by this author

Shuo-Yao Qu,

Shuo-Yao Qu

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

These authors contributed equally to this study.Search for more papers by this author

Hai-Feng Ou-yang,

Hai-Feng Ou-yang

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

These authors contributed equally to this study.Search for more papers by this author

Ya-Long He,

Ya-Long He

Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China

Search for more papers by this author

Zhi-Kui Li,

Zhi-Kui Li

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

Search for more papers by this author

Jie-Ran Shi,

Jie-Ran Shi

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

Search for more papers by this author

Li-Qiang Song,

Li-Qiang Song

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

Search for more papers by this author

Chang-Gui Wu,

Corresponding Author

Chang-Gui Wu

Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China

First published: 23 December 2013

https://doi.org/10.1111/resp.12198

Citations: 3

(Associate Editor: Darryl Knight).

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Abstract

Background and objective

Previous studies have demonstrated that our recombinant bacille Calmette-Guerin (rBCG), which expresses Der p2 in house dust mite (Der p2 rBCG) suppresses asthmatic airway inflammation by regulating the phenotype and function of dendritic cells (DC) and reprogramming T helper (Th) 0 cell differentiation into different T cell (Th1/Th2/Treg) subtypes. However, the exact role of Der p2 rBCG in reprogramming Th17 differentiation and the relevant mechanisms are not known. The aim of this study was to examine whether Der p2 rBCG-mediated inhibition of allergic airway inflammation is mediated by regulating Th17 differentiation in a murine asthma model.

Methods

Primary mouse bone marrow-derived dendritic cells (BMDC) were infected with Der p2 rBCG and adoptively transferred to Der p2-intranasally sensitized mice. The role of Der p2 rBCG-BMDC on the regulation of airway inflammation and Th17 cell differentiation was assessed.

Results

Adoptive transfer of Der p2 rBCG-BMDC suppressed airway inflammation and mucin secretion. Der p2 rBCG-BMDC inhibited excessive Th17 immune responses but not BCG-BMDC. Furthermore, Der p2 rBCG decreased jagged-2 and increased delta-like-4 expressions on BMDC to a greater extent than BCG.

Conclusions

These findings suggest that DC plays a key role in Der p2 rBCG-induced immunoregulation. Der p2 rBCG also displayed a potent inhibitory effect on Th17 differentiation, and these findings increase our understanding of the cellular basis of Der p2 BCG-mediated inhibition of asthma.

Supporting Information

References

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Citing Literature

Volume19, Issue1

January 2014

Pages 122-131

Der p2 recombinant bacille Calmette-Guerin priming of bone marrow-derived dendritic cells suppresses Der p2-induced T helper17 function in a mouse model of asthma

Abstract

Background and objective

Methods

Results

Conclusions

Supporting Information

References

Citing Literature

References

Information

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Der p2 recombinant bacille Calmette-Guerin priming of bone marrow-derived dendritic cells suppresses Der p2-induced T helper17 function in a mouse model of asthma

Abstract

Background and objective

Methods

Results

Conclusions

Supporting Information

References

Citing Literature

References

Related

Information