Associations among the largest follicle, preovulatory estradiol concentrations, and predominant vaginal epithelial cells at the completion of hormonal ovarian stimulation for fixed-time artificial insemination in goats
Nalinthip Promsao
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorPunnawut Yama
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Science and Technology Park, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorAssawadet Suriard
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorWichayaporn Butmata
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorRaktham Mektrirat
Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorJulakorn Panatuk
Faculty of Animal Science and Technology, Maejo University, Chiang Mai, Thailand
Search for more papers by this authorWilasinee Inyawilert
Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand
Search for more papers by this authorPayungsuk Intawicha
Unit of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand
Search for more papers by this authorJakree Jitjumnong
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorChien-Kai Wang
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan
The iEGG and Animal Biotechnology Center, Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan
Search for more papers by this authorCorresponding Author
Tossapol Moonmanee
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Functional Feed Innovation Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Correspondence
Tossapol Moonmanee, Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand.
Email: [email protected]
Search for more papers by this authorNalinthip Promsao
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorPunnawut Yama
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Science and Technology Park, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorAssawadet Suriard
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorWichayaporn Butmata
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorRaktham Mektrirat
Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorJulakorn Panatuk
Faculty of Animal Science and Technology, Maejo University, Chiang Mai, Thailand
Search for more papers by this authorWilasinee Inyawilert
Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand
Search for more papers by this authorPayungsuk Intawicha
Unit of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand
Search for more papers by this authorJakree Jitjumnong
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Search for more papers by this authorChien-Kai Wang
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan
The iEGG and Animal Biotechnology Center, Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan
Search for more papers by this authorCorresponding Author
Tossapol Moonmanee
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Functional Feed Innovation Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand
Correspondence
Tossapol Moonmanee, Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand.
Email: [email protected]
Search for more papers by this authorAbstract
The objective of the present study was to investigate the association among the largest follicle (LF), preovulatory estradiol (E2), and predominant vaginal epithelial cell at the completion of hormonal ovarian stimulation for fixed-time artificial insemination (FTAI) in goats. Thirty-seven crossbred Boer does received gonadotropin-releasing hormone (GnRH) and intravaginal progesterone (P4)-releasing devices (day 0). On day 5, P4 devices were removed and does received prostaglandin F2α and equine chorionic gonadotrophin. On day 7, does received GnRH, and FTAI was undertaken. On day 7, does were divided into three groups, i.e. small-sized (3–3.9 mm; n = 5), medium-sized (4–4.9 mm; n = 8), and large-sized (≥5 mm; n = 24) according to the diameter of the ovarian LF; follicular characteristics (number and diameter) were identified, and blood samples and vaginal smears were collected. The average diameters of total antral follicles and LF and the percentage of superficial cell were greatest in large-sized LF does (p < .01). The average diameters of total antral follicle (r = .68) and LF (r = .71), number of preovulatory follicle (r = .58), and plasma E2 concentrations (r = .61) were positively correlated with the percentage of superficial cells (p < .01). The likelihood of a pregnancy outcome after the FTAI increased by 13.71 times in does with a greater average diameter of antral follicle, 14.18 times with emergence of a large preovulatory follicle, and 36.83 times with a higher percentage of vaginal superficial cells (p < .01). It was concluded that there is a relationship between the cell types of the vaginal epithelium, the diameters of the largest ovarian follicles, and the concentration of E2 in goats subjected to FTAI protocols.
CONFLICT OF INTEREST STATEMENT
None of the authors have any conflict of interest to declare.
Open Research
DATA AVAILABILITY STATEMENT
The data generated during the current study are available from Tossapol Moonmanee upon reasonable request.
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