Volume 59, Issue 8 e14698
ORIGINAL ARTICLE

Associations among the largest follicle, preovulatory estradiol concentrations, and predominant vaginal epithelial cells at the completion of hormonal ovarian stimulation for fixed-time artificial insemination in goats

Nalinthip Promsao

Nalinthip Promsao

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

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Punnawut Yama

Punnawut Yama

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

Science and Technology Park, Chiang Mai University, Chiang Mai, Thailand

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Assawadet Suriard

Assawadet Suriard

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

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Wichayaporn Butmata

Wichayaporn Butmata

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

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Raktham Mektrirat

Raktham Mektrirat

Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand

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Julakorn Panatuk

Julakorn Panatuk

Faculty of Animal Science and Technology, Maejo University, Chiang Mai, Thailand

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Wilasinee Inyawilert

Wilasinee Inyawilert

Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand

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Payungsuk Intawicha

Payungsuk Intawicha

Unit of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand

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Jakree Jitjumnong

Jakree Jitjumnong

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

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Chien-Kai Wang

Chien-Kai Wang

Department of Animal Science, National Chung Hsing University, Taichung, Taiwan

The iEGG and Animal Biotechnology Center, Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan

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Tossapol Moonmanee

Corresponding Author

Tossapol Moonmanee

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

Functional Feed Innovation Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand

Correspondence

Tossapol Moonmanee, Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand.

Email: [email protected]

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First published: 29 July 2024
Citations: 1

Abstract

The objective of the present study was to investigate the association among the largest follicle (LF), preovulatory estradiol (E2), and predominant vaginal epithelial cell at the completion of hormonal ovarian stimulation for fixed-time artificial insemination (FTAI) in goats. Thirty-seven crossbred Boer does received gonadotropin-releasing hormone (GnRH) and intravaginal progesterone (P4)-releasing devices (day 0). On day 5, P4 devices were removed and does received prostaglandin F and equine chorionic gonadotrophin. On day 7, does received GnRH, and FTAI was undertaken. On day 7, does were divided into three groups, i.e. small-sized (3–3.9 mm; n = 5), medium-sized (4–4.9 mm; n = 8), and large-sized (≥5 mm; n = 24) according to the diameter of the ovarian LF; follicular characteristics (number and diameter) were identified, and blood samples and vaginal smears were collected. The average diameters of total antral follicles and LF and the percentage of superficial cell were greatest in large-sized LF does (p < .01). The average diameters of total antral follicle (r = .68) and LF (r = .71), number of preovulatory follicle (r = .58), and plasma E2 concentrations (r = .61) were positively correlated with the percentage of superficial cells (p < .01). The likelihood of a pregnancy outcome after the FTAI increased by 13.71 times in does with a greater average diameter of antral follicle, 14.18 times with emergence of a large preovulatory follicle, and 36.83 times with a higher percentage of vaginal superficial cells (p < .01). It was concluded that there is a relationship between the cell types of the vaginal epithelium, the diameters of the largest ovarian follicles, and the concentration of E2 in goats subjected to FTAI protocols.

CONFLICT OF INTEREST STATEMENT

None of the authors have any conflict of interest to declare.

DATA AVAILABILITY STATEMENT

The data generated during the current study are available from Tossapol Moonmanee upon reasonable request.

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