One panel with four single nucleotide polymorphisms for Chinese children with asthma: Integrating public data and whole exome sequencing
Ying Zhou
Department of Pediatrics Laboratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi, China
Contribution: Investigation, Funding acquisition, Writing - original draft, Methodology, Validation
Search for more papers by this authorLin Li
Oxford Suzhou Centre for Advanced Research, Suzhou, China
Contribution: Writing - original draft, Methodology, Investigation, Validation, Visualization
Search for more papers by this authorDongmei Zhou
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Contribution: Investigation, Methodology, Validation, Visualization, Software, Formal analysis
Search for more papers by this authorZhiwei Yu
Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi, China
Contribution: Investigation, Funding acquisition, Resources, Data curation, Methodology
Search for more papers by this authorYaning Ren
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Contribution: Investigation, Methodology, Validation, Visualization
Search for more papers by this authorYuanfen Liao
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Contribution: Investigation, Software, Formal analysis, Data curation
Search for more papers by this authorCunyin Yuan
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Contribution: Investigation, Formal analysis, Software, Data curation, Resources
Search for more papers by this authorCorresponding Author
Yong Yin
Department of Respiratory, Shanghai Children's Medical Center, Affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, China
Correspondence
Yong Yin, Department of Respiratory, Shanghai Children's Medical Center, Affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, China.
Email: [email protected]
Xiaohong Gu, Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi 214023, China.
Email: [email protected]
Yubao Cui, Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi 214023, China.
Email: [email protected] and [email protected]
Contribution: Resources, Project administration, Conceptualization, Writing - review & editing
Search for more papers by this authorCorresponding Author
Xiaohong Gu
Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi, China
Correspondence
Yong Yin, Department of Respiratory, Shanghai Children's Medical Center, Affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, China.
Email: [email protected]
Xiaohong Gu, Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi 214023, China.
Email: [email protected]
Yubao Cui, Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi 214023, China.
Email: [email protected] and [email protected]
Contribution: Supervision, Project administration, Data curation, Resources, Writing - review & editing
Search for more papers by this authorCorresponding Author
Yubao Cui
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Correspondence
Yong Yin, Department of Respiratory, Shanghai Children's Medical Center, Affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, China.
Email: [email protected]
Xiaohong Gu, Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi 214023, China.
Email: [email protected]
Yubao Cui, Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi 214023, China.
Email: [email protected] and [email protected]
Contribution: Conceptualization, Writing - review & editing, Resources, Supervision, Project administration
Search for more papers by this authorYing Zhou
Department of Pediatrics Laboratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi, China
Contribution: Investigation, Funding acquisition, Writing - original draft, Methodology, Validation
Search for more papers by this authorLin Li
Oxford Suzhou Centre for Advanced Research, Suzhou, China
Contribution: Writing - original draft, Methodology, Investigation, Validation, Visualization
Search for more papers by this authorDongmei Zhou
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Contribution: Investigation, Methodology, Validation, Visualization, Software, Formal analysis
Search for more papers by this authorZhiwei Yu
Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi, China
Contribution: Investigation, Funding acquisition, Resources, Data curation, Methodology
Search for more papers by this authorYaning Ren
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Contribution: Investigation, Methodology, Validation, Visualization
Search for more papers by this authorYuanfen Liao
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Contribution: Investigation, Software, Formal analysis, Data curation
Search for more papers by this authorCunyin Yuan
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Contribution: Investigation, Formal analysis, Software, Data curation, Resources
Search for more papers by this authorCorresponding Author
Yong Yin
Department of Respiratory, Shanghai Children's Medical Center, Affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, China
Correspondence
Yong Yin, Department of Respiratory, Shanghai Children's Medical Center, Affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, China.
Email: [email protected]
Xiaohong Gu, Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi 214023, China.
Email: [email protected]
Yubao Cui, Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi 214023, China.
Email: [email protected] and [email protected]
Contribution: Resources, Project administration, Conceptualization, Writing - review & editing
Search for more papers by this authorCorresponding Author
Xiaohong Gu
Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi, China
Correspondence
Yong Yin, Department of Respiratory, Shanghai Children's Medical Center, Affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, China.
Email: [email protected]
Xiaohong Gu, Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi 214023, China.
Email: [email protected]
Yubao Cui, Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi 214023, China.
Email: [email protected] and [email protected]
Contribution: Supervision, Project administration, Data curation, Resources, Writing - review & editing
Search for more papers by this authorCorresponding Author
Yubao Cui
Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China
Correspondence
Yong Yin, Department of Respiratory, Shanghai Children's Medical Center, Affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, China.
Email: [email protected]
Xiaohong Gu, Department of Respiratory, The Affiliated Children's Hospital of Jiangnan University, Wuxi 214023, China.
Email: [email protected]
Yubao Cui, Clinical Research Center, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi 214023, China.
Email: [email protected] and [email protected]
Contribution: Conceptualization, Writing - review & editing, Resources, Supervision, Project administration
Search for more papers by this authorYing Zhou and Lin Li contributed equally to this work.
Abstract
Background
Polymorphisms in susceptibility genes are a major risk factor for the development of asthma. Understanding these genetic variants helps elucidate asthma's pathogenesis, predict its onset, expedite antiasthma medication development, and achieve precise targeted individualized treatment. This study developed a test kit based on susceptibility genes for predicting asthma in Chinese children.
Methods
The present study constructed a VariantPro Targeted Library Preparation System with 72 single nucleotide polymorphism (SNP) loci associated with asthma from the ClinVar, OMIM, and SNPedia databases. These SNP loci were detected in the peripheral blood of 499 children with asthma and 500 healthy children. Significant differences were discovered for seven SNP loci. Simultaneously, whole exome sequencing of 46 children with asthma and 50 healthy children identified eight SNP loci with significant differences. The 15 SNP loci identified from Chinese children with asthma were validated in an independent population of 97 children with asthma and 93 healthy children by conducting multiplex polymerase chain reaction (PCR)–next-generation sequencing genotyping.
Results
Four loci (rs12422149, rs7216389, rs4065275, and rs41453444) were identified, and a single-tube multifluorescent qPCR (real-time quantitative PCR) test kit was developed using these four SNP loci. The kit was tested on 269 children with asthma and 724 children with bronchopneumonia.
Conclusions
We identified four loci as susceptibility genes and developed a quantitative PCR test kit for predicting asthma development in Chinese children.
CONFLICT OF INTEREST STATEMENT
All the authors declare no conflicts of interest with respect to the content of this article. The authors declare no affiliations with or financial involvement in organizations or entities with a direct financial interest in the subject matter or materials discussed in the manuscript.
Open Research
DATA AVAILABILITY STATEMENT
Data sharing is not applicable to this article because no new data were created or analyzed in this study.
Supporting Information
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Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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