Effect of orodispersible hyaluronic acid film on palatal mucosa wound healing
Jeong Hyun Lee
Department of Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea
Contribution: Data curation, Formal analysis, Writing - original draft
Search for more papers by this authorKo Eun Lee
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, South Korea
Contribution: Validation, Writing - original draft, Writing - review & editing
Search for more papers by this authorSang Wook Kang
Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul, South Korea
Contribution: Data curation, Formal analysis
Search for more papers by this authorSeung Hwan Park
Department of Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea
Contribution: Data curation, Formal analysis
Search for more papers by this authorYong Kwon Chae
Department of Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, South Korea
Contribution: Writing - original draft
Search for more papers by this authorMyoung-Han Lee
Jinwoo Bio Co. Ltd., Yongin-si, South Korea
Contribution: Data curation, Formal analysis, Resources
Search for more papers by this authorDong-Keun Kweon
Jinwoo Bio Co. Ltd., Yongin-si, South Korea
Contribution: Data curation, Formal analysis, Resources
Search for more papers by this authorSung Chul Choi
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, South Korea
Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, South Korea
Contribution: Validation
Search for more papers by this authorCorresponding Author
Ok Hyung Nam
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, South Korea
Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, South Korea
Correspondence
Ok Hyung Nam, Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Kyungheedae-Ro 26, Dongdaemoon-Gu, Seoul 02447, South Korea.
Email: [email protected]
Contribution: Conceptualization, Data curation, Validation, Formal analysis, Funding acquisition, Writing - original draft, Writing - review & editing
Search for more papers by this authorJeong Hyun Lee
Department of Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea
Contribution: Data curation, Formal analysis, Writing - original draft
Search for more papers by this authorKo Eun Lee
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, South Korea
Contribution: Validation, Writing - original draft, Writing - review & editing
Search for more papers by this authorSang Wook Kang
Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul, South Korea
Contribution: Data curation, Formal analysis
Search for more papers by this authorSeung Hwan Park
Department of Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea
Contribution: Data curation, Formal analysis
Search for more papers by this authorYong Kwon Chae
Department of Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, South Korea
Contribution: Writing - original draft
Search for more papers by this authorMyoung-Han Lee
Jinwoo Bio Co. Ltd., Yongin-si, South Korea
Contribution: Data curation, Formal analysis, Resources
Search for more papers by this authorDong-Keun Kweon
Jinwoo Bio Co. Ltd., Yongin-si, South Korea
Contribution: Data curation, Formal analysis, Resources
Search for more papers by this authorSung Chul Choi
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, South Korea
Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, South Korea
Contribution: Validation
Search for more papers by this authorCorresponding Author
Ok Hyung Nam
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, South Korea
Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, South Korea
Correspondence
Ok Hyung Nam, Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Kyungheedae-Ro 26, Dongdaemoon-Gu, Seoul 02447, South Korea.
Email: [email protected]
Contribution: Conceptualization, Data curation, Validation, Formal analysis, Funding acquisition, Writing - original draft, Writing - review & editing
Search for more papers by this authorJeong Hyun Lee and Ko Eun Lee contributed equally to this work as first authors.
Abstract
Objective
The objective of the study was to evaluate the healing effect of hyaluronic acid films on palatal wounds.
Materials and Methods
After making 5-mm diameter palatal wounds, 72 rats were randomly assigned to three groups: control, hyaluronic acid gel, and hyaluronic acid film. The animals were sacrificed at 3, 7, and 21 days after the experiment. Clinical, histological, and RT-PCR analyses were performed. Human ex vivo oral mucosa models were used. Histological analysis and pan-cytokeratin staining were performed at 5 days after wound creation.
Results
In rat model, both gels and films showed favorable healing on Days 3 and 7 compared with healing in the control (p < 0.05). Film showed remarkable VEGF and α-SMA expression than did the others (p < 0.05). Immunohistochemical analysis showed that film exhibited significantly lower CD68 and greater α-SMA and vimentin expression levels than those in the others (p < 0.05). In human model, re-epithelialization rate of film group was significantly higher than that of the others. Complete epithelial regeneration was confirmed only in film group using pan-cytokeratin staining.
Conclusions
Within the limits of this study, hyaluronic acid film outperformed gels in terms of palatal wound healing in both models.
CONFLICT OF INTEREST STATEMENT
The authors report that there are no direct or indirect conflicts of interest involving associations with commercial entities that supported the work reported in the submitted manuscript, associations with commercial entities that might have an interest in the submitted manuscript, financial associations involving family members and other relevant non-financial associations.
Open Research
DATA AVAILABILITY STATEMENT
Datasets related to this article will be available upon request to the corresponding author.
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