Combined signatures of serum proteome and transcriptome in patients with recurrent aphthous ulcer
Jie Bao
School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Conceptualization, Data curation, Writing - original draft
Search for more papers by this authorJuan Chen
School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Conceptualization, Formal analysis, Writing - original draft
Search for more papers by this authorXiZhao Zhang
School of 1st Clinical Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Data curation, Methodology
Search for more papers by this authorLi Xu
School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Conceptualization
Search for more papers by this authorYongSheng Fan
School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Conceptualization, Project administration
Search for more papers by this authorCorresponding Author
XiaoBing Dou
School of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Correspondence
XiaoBing Dou, School of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China.
Email: [email protected].
Contribution: Conceptualization, Data curation, Writing - review & editing
Search for more papers by this authorJie Bao
School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Conceptualization, Data curation, Writing - original draft
Search for more papers by this authorJuan Chen
School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Conceptualization, Formal analysis, Writing - original draft
Search for more papers by this authorXiZhao Zhang
School of 1st Clinical Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Data curation, Methodology
Search for more papers by this authorLi Xu
School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Conceptualization
Search for more papers by this authorYongSheng Fan
School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Contribution: Conceptualization, Project administration
Search for more papers by this authorCorresponding Author
XiaoBing Dou
School of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, China
Correspondence
XiaoBing Dou, School of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China.
Email: [email protected].
Contribution: Conceptualization, Data curation, Writing - review & editing
Search for more papers by this authorJie Bao, Juan Chen contributed equally to the paper as first authors.
Abstract
Objectives
Recurrent aphthous ulcer (RAU) is a common oral disease with unclear mechanism. This study aimed to explore the serum signatures of RAU patients via proteomic and transcriptomic analysis.
Methods
This study was based on clinical observation. Part of serum was used for clinical tests, while the rest was processed for isobaric tags for relative and absolute quantitation (ITRAQ) labeling coupled with two-dimensional liquid chromatography–tandem mass spectrometry (2D LC-MS/MS) combined with microRNA (miRNA) microarrays. Bioinformatic analysis was then used to obtain significant signatures, which was verified by ELISA, qRT-PCR, and dual-luciferase reporter gene assays.
Results
Clinical data showed that triglyceride level, white blood cell count, and neutrophils percentage were increased in RAU group, while lymphocytes percentage was decreased. ITRAQ-2D LC-MS/MS identified 22 upregulated and 33 downregulated proteins in RAU group. Simultaneously, miRNA microarrays identified 64 upregulated and 31 downregulated miRNAs. After integrative bioinformatic analysis and verification, three miRNA-protein pairs, mainly involved in oxidative stress and inflammation responses, were obtained. Additionally, the interaction network indicated the crucial role of complement and coagulation cascade pathway in RAU.
Conclusions
Our study revealed that complement and coagulation cascade pathway, oxidative stress, and inflammation responses may act as vital factors in pathogenesis of RAU.
CONFLICT OF INTEREST
All authors declared no potential conflicts of interest with respect to the authorship and publication of this manuscript.
Supporting Information
| Filename | Description |
|---|---|
| odi13800-sup-0001-FigS1.tifTIFF image, 1.3 MB | Fig S1 |
| odi13800-sup-0002-FigS2.tifTIFF image, 5.2 MB | Fig S2 |
| odi13800-sup-0003-TableS1-S2.docxWord 2007 document , 25.2 KB | Table S1-S2 |
| odi13800-sup-0004-Supinfo.xlsxExcel 2007 spreadsheet , 179.3 KB | Supplementary Material |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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