FAK inhibition protects condylar cartilage under excessive mechanical stress
Makoto Yanoshita
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Data curation, Formal analysis, Investigation, Visualization, Writing - original draft
Search for more papers by this authorCorresponding Author
Naoto Hirose
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Correspondence
Naoto Hirose, Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Kasumi 1-2-3 Minami-ku, Hiroshima-shi, Hiroshima prefecture, Japan.
Email: [email protected]
Contribution: Conceptualization, Methodology, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorChikako Sumi
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorMami Takano
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorSayuri Nishiyama
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorEri Tsuboi
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorAzusa Onishi
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorYuka Yamauchi
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorYuki Asakawa
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Data curation
Search for more papers by this authorKunimatsu Ryo
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Data curation
Search for more papers by this authorKotaro Tanimoto
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Conceptualization, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorMakoto Yanoshita
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Data curation, Formal analysis, Investigation, Visualization, Writing - original draft
Search for more papers by this authorCorresponding Author
Naoto Hirose
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Correspondence
Naoto Hirose, Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Kasumi 1-2-3 Minami-ku, Hiroshima-shi, Hiroshima prefecture, Japan.
Email: [email protected]
Contribution: Conceptualization, Methodology, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorChikako Sumi
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorMami Takano
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorSayuri Nishiyama
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorEri Tsuboi
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorAzusa Onishi
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorYuka Yamauchi
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Investigation
Search for more papers by this authorYuki Asakawa
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Data curation
Search for more papers by this authorKunimatsu Ryo
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Data curation
Search for more papers by this authorKotaro Tanimoto
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima prefecture, Japan
Contribution: Conceptualization, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorAbstract
Objectives
Excessive mechanical stress is assumed to be a major cause of temporomandibular joint (TMJ) osteoarthritis (OA). +Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase involved in a variety of signaling pathways. Little has been reported on the function of FAK in TMJ-OA. In the present study, we investigated the effect of FAK inhibition on TMJ cartilage under excessive mechanical loading stress.
Materials and Methods
Articular cartilage explants were harvested from the TMJ of rats and subjected to mechanical loading in the presence of an FAK inhibitor in organ culture. The gene expression of inflammatory cytokines was examined after the application of mechanical loading with or without FAK inhibitor. Paraffin-embedded sections of articular cartilage were stained with hematoxylin and eosin, safranin O and fast Green, toluidine blue, TUNEL staining, and immunohistochemical staining and was performed to investigate the protein expression of IL-1β and MMP-13.
Results
Treatment with FAK inhibitor reduced the gene expression of inflammatory cytokines and inhibited the degradation of articular cartilage, as determined histologically. FAK inhibitor treatment also suppressed the protein expression of IL-1β and MMP-13 in the hypertrophic zone, as determined immunohistologically.
Conclusion
Treatment with FAK inhibitor suppresses inflammation and protects condylar cartilage under excessive mechanical loading.
Supporting Information
| Filename | Description |
|---|---|
| odi13494-sup-0001-FigS1.tiffTIFF image, 859.6 KB | Fig S1 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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