Benzo[a]pyrene promotes progression in tongue squamous cell carcinoma
Linxuan Huang
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Writing - original draft, Writing - review & editing
Search for more papers by this authorXiaofen Xiao
Department of Stomatology, Shenzhen People’s Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen, China
Contribution: Funding acquisition, Resources, Writing - review & editing
Search for more papers by this authorYupeng Yao
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Contribution: Methodology, Validation
Search for more papers by this authorJianping Yu
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Contribution: Data curation, Software
Search for more papers by this authorQingjian Chen
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Contribution: Data curation, Methodology
Search for more papers by this authorCorresponding Author
Puping Liang
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Correspondence
Puping Liang and Yan Zhang, Guangzhou Higher Education Mega Center, Guangzhou 510006, P. R. China.
Emails: [email protected] (P. L.); [email protected] (Y. Z.)
Search for more papers by this authorCorresponding Author
Yan Zhang
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Correspondence
Puping Liang and Yan Zhang, Guangzhou Higher Education Mega Center, Guangzhou 510006, P. R. China.
Emails: [email protected] (P. L.); [email protected] (Y. Z.)
Contribution: Conceptualization, Data curation, Funding acquisition, Project administration, Resources, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing
Search for more papers by this authorLinxuan Huang
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Writing - original draft, Writing - review & editing
Search for more papers by this authorXiaofen Xiao
Department of Stomatology, Shenzhen People’s Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen, China
Contribution: Funding acquisition, Resources, Writing - review & editing
Search for more papers by this authorYupeng Yao
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Contribution: Methodology, Validation
Search for more papers by this authorJianping Yu
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Contribution: Data curation, Software
Search for more papers by this authorQingjian Chen
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Contribution: Data curation, Methodology
Search for more papers by this authorCorresponding Author
Puping Liang
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Correspondence
Puping Liang and Yan Zhang, Guangzhou Higher Education Mega Center, Guangzhou 510006, P. R. China.
Emails: [email protected] (P. L.); [email protected] (Y. Z.)
Search for more papers by this authorCorresponding Author
Yan Zhang
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Correspondence
Puping Liang and Yan Zhang, Guangzhou Higher Education Mega Center, Guangzhou 510006, P. R. China.
Emails: [email protected] (P. L.); [email protected] (Y. Z.)
Contribution: Conceptualization, Data curation, Funding acquisition, Project administration, Resources, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing
Search for more papers by this authorLinxuan Huang and Xiaofen Xiao contributed equally to this work.
Funding information
This work was supported by grants from Guangdong Provincial Key Scientific and Technological Projects (2017B020230002 and 2016B030231001) and National Natural Science Foundation of China (No. 31871413), and the Shenzhen Science and Technology Project (JCYJ20160422144656321).
Abstract
Objectives
Benzo[a]pyrene (B[a]P) is a member of the polycyclic aromatic hydrocarbon (PAH) family. Although the potent carcinogenicity of high-dose B[a]P has been extensively reported, the effects of long-term exposure to B[a]P on the progression of tongue squamous cell carcinoma (TSCC) are poorly understood.
Methods
In the present study, TSCC cells were treated with 5 or 50 nM of B[a]P for three months. The proliferation and chemoresistance of B[a]P-treated cells to 5-fluorouracil or cisplatin were detected by CCK8. The motility of the B[a]P-treated cells was evaluated with wound healing analysis, invasion assay, and three-dimensional culture in decellularized mouse tongue matrix. Xenograft assay was used to investigate the aggressiveness of B[a]P-treated cells. Immunofluorescence staining, terminal restriction fragment assay, and whole-genome sequence were used to determine the mutation spectrums.
Results
Long-term 50 nM B[a]P-treated cells exhibited increased aggressiveness and chemoresistance to 5-fluorouracil or cisplatin. In addition, data from whole-genome sequencing demonstrated that C:T to A:T transitions were the predominant nucleotide substitutions occurred in 50 nM B[a]P-treated CAL27 cells. Furthermore, 102 non-synonymous or stop-gain mutations were enriched in the extracellular-matrix-receptor interactive pathway.
Conclusions
B[a]P exposure may contribute to genomic instability, and therefore, B[a]P may promote the progression of TSCC.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
Supporting Information
| Filename | Description |
|---|---|
| odi13489-sup-0001-TableS1.pdfPDF document, 309.9 KB | Table S1 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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