Evaluation of PD-L1, PD-L2, PD-1 and cytotoxic immune response in oral lichen planus
Corresponding Author
Nádia Lago Costa
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Correspondence
Nádia Lago Costa, Department of Stomatology (Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Praça Universitária, S/N, Setor Universitário, Goiânia, GO, CEP: 74605-220, Brazil.
Emails: [email protected]; [email protected]
Search for more papers by this authorJulie Ane Maria Gonçalves
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Search for more papers by this authorSara Lia Gonçalves de Lima
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Search for more papers by this authorJosé Alcides Almeida de Arruda
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Search for more papers by this authorAna Carolina Campos Miranda
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Search for more papers by this authorRicardo Alves Mesquita
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Search for more papers by this authorÉricka Janine Dantas da Silveira
Postgraduate Program in Odontology Sciences, Department of Dentistry, Universidade Federal do Rio Grande do Norte, Natal, Brazil
Search for more papers by this authorAline Carvalho Batista
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Search for more papers by this authorCorresponding Author
Nádia Lago Costa
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Correspondence
Nádia Lago Costa, Department of Stomatology (Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Praça Universitária, S/N, Setor Universitário, Goiânia, GO, CEP: 74605-220, Brazil.
Emails: [email protected]; [email protected]
Search for more papers by this authorJulie Ane Maria Gonçalves
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Search for more papers by this authorSara Lia Gonçalves de Lima
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Search for more papers by this authorJosé Alcides Almeida de Arruda
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Search for more papers by this authorAna Carolina Campos Miranda
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Search for more papers by this authorRicardo Alves Mesquita
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Search for more papers by this authorÉricka Janine Dantas da Silveira
Postgraduate Program in Odontology Sciences, Department of Dentistry, Universidade Federal do Rio Grande do Norte, Natal, Brazil
Search for more papers by this authorAline Carvalho Batista
Department of Stomatology (Oral Medicine and Oral Pathology), School of Dentistry, Universidade Federal de Goiás, Goiânia, Brazil
Search for more papers by this authorAbstract
Objective
To investigate the expression of programmed death-ligands 1 and 2 (PD-L1, PD-L2), programmed death-1 (PD-1), CD8 and granzyme B (GrB), as well as its correlation with the severity of oral lichen planus (OLP).
Materials and Methods
In a collaborative study, 33 cases of OLP were evaluated according to the latest criteria proposed by the American Academy of Oral and Maxillofacial Pathology (AAOMP/2016) and were submitted to immunohistochemistry. Positivity was measured semiquantitatively (PD-L1, PD-L2) and quantitatively (PD-1, CD8, GrB). The severity of OLP was assessed according to clinical subtype, symptomatology and response to corticosteroid therapy.
Results
Most OLPs were considered to be negative for PD-L1 (66.6%), but high expression of PD-L2 (96.9%) by keratinocytes and immunoinflammatory cells was observed. PD-1+ cell density/mm2 was reduced compared to CD8+ cells. A low cytotoxic immune response (CD8:GrB ratio) was also demonstrated. Interestingly, there were fewer GrB+ cells in the intraepithelial region in reticular OLP compared to erosive/bullous OLP.
Conclusions
PD-L1/PD-1 pathways appear to be compromised in OLP due to low PD-L1 expression in most samples. In contrast, PD-L2 overexpression associated with a possible regulation of the cytotoxic immune response suggests an immune tolerance that may contribute to the chronic profile of OLP.
CONFLICT OF INTERESTS
None to declare.
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