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Retracted: Cytokines induce MMP-3-regulated proliferation of embryonic stem cell-derived odontoblast-like cells

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Corresponding Author

N Ozeki

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

Correspondence: Nobuaki Ozeki, Department of Endodontics, School of Dentistry, Aichi Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan. Tel: +81 52 759 2148, Fax: +81 52 764 2299, E-mail: [email protected]Search for more papers by this author

H Yamaguchi,

H Yamaguchi

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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R Kawai,

R Kawai

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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T Hiyama,

T Hiyama

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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K Nakata,

K Nakata

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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M Mogi,

M Mogi

Department of Medicinal Biochemistry, School of Pharmacy, Aichi Gakuin University, Nagoya, Japan

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H Nakamura,

H Nakamura

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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N Ozeki,

Corresponding Author

N Ozeki

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

H Yamaguchi,

H Yamaguchi

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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R Kawai,

R Kawai

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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T Hiyama,

T Hiyama

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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K Nakata,

K Nakata

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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M Mogi,

M Mogi

Department of Medicinal Biochemistry, School of Pharmacy, Aichi Gakuin University, Nagoya, Japan

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H Nakamura,

H Nakamura

Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi, Japan

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First published: 13 July 2013

https://doi.org/10.1111/odi.12165

Citations: 14

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Abstract

Objectives

Matrix metalloproteinase (MMP)-3 expression increases after pulpectomy and accelerates angiogenesis in rat dental pulp by an uncharacterised mechanism. Odontoblasts, a major component of dental pulp, could represent a therapeutic target. We investigated whether MMP-3 activity is induced by cytokines and/or is associated with cell proliferation and apoptosis in embryonic stem cell-derived odontoblast-like cells.

Materials and Methods

We used reverse transcriptase polymerase chain reaction, western blotting, an MMP-3 activity assay, a BrdU-cell proliferation enzyme-linked immunosorbent assay and DNA fragmentation analysis to evaluate siRNA-mediated downregulation of MMP-3 expression and activity, and any changes in the proliferative and apoptotic responses associated with this reduced expression.

Results

Pro-inflammatory cytokines (interleukin-1β, tumour necrosis factor-α and interferon-γ, at relatively low concentrations) induced MMP-3 mRNA and protein expression, and increased MMP-3 activity and cell proliferation, but not apoptosis. MMP-3 silencing produced a potent and significant suppression of cytokine-induced MMP-3 expression and activity, decreased cell proliferation and increased apoptosis. These effects were rescued by application of exogenous MMP-3.

Conclusions

Our results suggest that pro-inflammatory cytokines induce MMP-3-regulated cell proliferation and anti-apoptosis effects in odontoblast-like cells derived from embryonic stem cells, in addition to their well-documented destructive role in inflammation.

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Volume20, Issue5

July 2014

Pages 505-513

Retracted: Cytokines induce MMP-3-regulated proliferation of embryonic stem cell-derived odontoblast-like cells

Retraction(s) for this article

Retraction statement: Cytokines induce MMP-3-regulated proliferation of embryonic stem cell-derived odontoblast-like cells

Abstract

Objectives

Materials and Methods

Results

Conclusions

References

Citing Literature

References

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Retracted: Cytokines induce MMP-3-regulated proliferation of embryonic stem cell-derived odontoblast-like cells

Retraction(s) for this article

Retraction statement: Cytokines induce MMP-3-regulated proliferation of embryonic stem cell-derived odontoblast-like cells

Abstract

Objectives

Materials and Methods

Results

Conclusions

References

Citing Literature

References

Related

Information