Effects of resveratrol on bone-healing capacity in the mouse tooth extraction socket
Kyung-Kon Min
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorSanjiv Neupane
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorNirpesh Adhikari
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorWern-Joo Sohn
Pre-Major of Cosmetics and Pharmaceutics, Daegu Haany University, Gyeongsan, Korea
Search for more papers by this authorSeo-Young An
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJi-Youn Kim
Department of Dental Hygiene, Gachon University, Incheon, Korea
Search for more papers by this authorChang-Hyeon An
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorYoungkyun Lee
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorYong-Gun Kim
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJin-Woo Park
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJae-Mok Lee
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorCorresponding Author
Jae-Young Kim
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Correspondence
Jo-Young Suh, Department of Periodontology, School of Dentistry, Kyungpook National University, 2177, Dalgubeol-daero, Jung-gu, Daegu 41940, Korea.
Email: [email protected]
Jae-Young Kim, Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, 2177, Dalgubeol-daero, Jung-gu, Daegu 41940, Korea.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jo-Young Suh
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Correspondence
Jo-Young Suh, Department of Periodontology, School of Dentistry, Kyungpook National University, 2177, Dalgubeol-daero, Jung-gu, Daegu 41940, Korea.
Email: [email protected]
Jae-Young Kim, Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, 2177, Dalgubeol-daero, Jung-gu, Daegu 41940, Korea.
Email: [email protected]
Search for more papers by this authorKyung-Kon Min
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorSanjiv Neupane
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorNirpesh Adhikari
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorWern-Joo Sohn
Pre-Major of Cosmetics and Pharmaceutics, Daegu Haany University, Gyeongsan, Korea
Search for more papers by this authorSeo-Young An
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJi-Youn Kim
Department of Dental Hygiene, Gachon University, Incheon, Korea
Search for more papers by this authorChang-Hyeon An
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorYoungkyun Lee
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorYong-Gun Kim
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJin-Woo Park
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJae-Mok Lee
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorCorresponding Author
Jae-Young Kim
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Correspondence
Jo-Young Suh, Department of Periodontology, School of Dentistry, Kyungpook National University, 2177, Dalgubeol-daero, Jung-gu, Daegu 41940, Korea.
Email: [email protected]
Jae-Young Kim, Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, 2177, Dalgubeol-daero, Jung-gu, Daegu 41940, Korea.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jo-Young Suh
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Correspondence
Jo-Young Suh, Department of Periodontology, School of Dentistry, Kyungpook National University, 2177, Dalgubeol-daero, Jung-gu, Daegu 41940, Korea.
Email: [email protected]
Jae-Young Kim, Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, 2177, Dalgubeol-daero, Jung-gu, Daegu 41940, Korea.
Email: [email protected]
Search for more papers by this authorAbstract
Background and Objective
After tooth extraction, the extraction socket undergoes several steps of soft and hard tissue healing. The healing process of the extraction socket is modulated by a range of signaling factors and biochemical agents. It has been reported that resveratrol, a polyphenolic compound, exhibits various biological effects, including anti-inflammatory, anti-carcinogenic, antioxidant, and anti-aging effects, and protects cardiovascular and bone tissues. In this study, we examined the cellular effects of resveratrol on human periodontal ligament (hPDL) cells and osteoblast-like (MC3T3-E1) cells and evaluated the bone-healing capacity of tooth extraction sockets in mice.
Material and Methods
Resveratrol was applied to hPDL and MC3T3-E1 cells to detect cell proliferation and alkaline phosphatase (ALP) activity, and qPCR was employed to understand the gene expression level in vitro. For in vivo experiment, six-week-old C57BL/6 male mice were randomly divided into control (n = 15) and experimental (n = 15) groups and maxillary first molars were extracted by surgery. Experimental groups received 50-µM resveratrol on extraction sockets and analyzed the degree of new bone formation.
Results
Treatment of hPDL and MC3T3-E1 cells with resveratrol increased the cell proliferation and ALP activity and enhanced the expression of ALP, BMP-2, BMP-4, and OC genes. Resveratrol enhanced new bone formation in the lingual extraction socket in mice.
Conclusion
These results suggest that resveratrol increases the cellular physiology of PDL and osteoblast including their proliferation and differentiation and may play an important role in bone-healing capacity after tooth extraction.
CONFLICT OF INTEREST
The authors declare no conflicts of interest.
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