A model of genital herpes simplex virus Type 1 infection in Rhesus Macaques
Meropi Aravantinou
Center for Biomedical Research, Population Council, New York, NY, USA
Search for more papers by this authorInes Frank
Center for Biomedical Research, Population Council, New York, NY, USA
Search for more papers by this authorGeraldine Arrode-Bruses
Center for Biomedical Research, Population Council, New York, NY, USA
Search for more papers by this authorMoriah Szpara
The Pennsylvania State University, University Park, PA, USA
Search for more papers by this authorBrooke Grasperge
Tulane National Primate Research Center, Tulane University, Covington, LA, USA
Search for more papers by this authorJames Blanchard
Tulane National Primate Research Center, Tulane University, Covington, LA, USA
Search for more papers by this authorAgegnehu Gettie
Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY, USA
Search for more papers by this authorNina Derby
Center for Biomedical Research, Population Council, New York, NY, USA
Search for more papers by this authorCorresponding Author
Elena Martinelli
Center for Biomedical Research, Population Council, New York, NY, USA
Correspondence
Elena Martinelli, Population Council, New York, NY, USA.
Email: [email protected]
Search for more papers by this authorMeropi Aravantinou
Center for Biomedical Research, Population Council, New York, NY, USA
Search for more papers by this authorInes Frank
Center for Biomedical Research, Population Council, New York, NY, USA
Search for more papers by this authorGeraldine Arrode-Bruses
Center for Biomedical Research, Population Council, New York, NY, USA
Search for more papers by this authorMoriah Szpara
The Pennsylvania State University, University Park, PA, USA
Search for more papers by this authorBrooke Grasperge
Tulane National Primate Research Center, Tulane University, Covington, LA, USA
Search for more papers by this authorJames Blanchard
Tulane National Primate Research Center, Tulane University, Covington, LA, USA
Search for more papers by this authorAgegnehu Gettie
Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY, USA
Search for more papers by this authorNina Derby
Center for Biomedical Research, Population Council, New York, NY, USA
Search for more papers by this authorCorresponding Author
Elena Martinelli
Center for Biomedical Research, Population Council, New York, NY, USA
Correspondence
Elena Martinelli, Population Council, New York, NY, USA.
Email: [email protected]
Search for more papers by this authorFunding information
This work was supported by NIH grants R01 AI098456-04 and OD011104.
Abstract
Background
Although HSV-2 is the major cause of genital lesions, HSV-1 accounts for half of new cases in developed countries.
Methods
Three healthy SHIV-SF162P3-infected Indian rhesus macaques were inoculated with 4×108 pfu of HSV-1 twice, with the second inoculation performed after the vaginal mucosa was gently abraded with a cytobrush.
Results
HSV-1 DNA was detected in vaginal swabs 5 days after the second but not the first inoculation in all three macaques. An increase in inflammatory cytokines was detected in the vaginal fluids of the animals with no or intermittent shedding. Higher frequency of blood α4β7high CD4+ T cells was measured in the animals with consistent and intermitted shedding, while a decrease in the frequency of CD69+ CD4+ T cells was present in all animals.
Conclusions
This macaque model of genital HSV-1 could be useful to study the impact of the growing epidemic of genital HSV-1 on HIV infection.
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