Dieckol isolated from a brown alga, Eisenia nipponica, suppresses ear swelling from allergic inflammation in mouse
Corresponding Author
Yoshimasa Sugiura
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Correspondence
Yoshimasa Sugiura, Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, 2-7-1 Nagata-Honmachi, Shimonoseki, Yamaguchi 759-6595, Japan.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Writing - original draft, Writing - review & editing
Search for more papers by this authorMasakatsu Usui
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Contribution: Data curation, Formal analysis, Investigation, Methodology, Writing - review & editing
Search for more papers by this authorHirotaka Katsuzaki
Laboratory of Bioorganic Chemistry, Graduate School of Bioresources, Mie University, Tsu, Japan
Contribution: Data curation, Formal analysis, Investigation, Methodology, Writing - review & editing
Search for more papers by this authorKunio Imai
Laboratory of Bioorganic Chemistry, Graduate School of Bioresources, Mie University, Tsu, Japan
Contribution: Data curation, Formal analysis, Investigation, Methodology, Writing - review & editing
Search for more papers by this authorRyusuke Tanaka
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Contribution: Methodology, Writing - review & editing
Search for more papers by this authorTeruo Matsushita
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Contribution: Data curation, Methodology, Writing - review & editing
Search for more papers by this authorMasaaki Miyata
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Contribution: Data curation, Methodology, Supervision, Writing - review & editing
Search for more papers by this authorCorresponding Author
Yoshimasa Sugiura
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Correspondence
Yoshimasa Sugiura, Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, 2-7-1 Nagata-Honmachi, Shimonoseki, Yamaguchi 759-6595, Japan.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Writing - original draft, Writing - review & editing
Search for more papers by this authorMasakatsu Usui
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Contribution: Data curation, Formal analysis, Investigation, Methodology, Writing - review & editing
Search for more papers by this authorHirotaka Katsuzaki
Laboratory of Bioorganic Chemistry, Graduate School of Bioresources, Mie University, Tsu, Japan
Contribution: Data curation, Formal analysis, Investigation, Methodology, Writing - review & editing
Search for more papers by this authorKunio Imai
Laboratory of Bioorganic Chemistry, Graduate School of Bioresources, Mie University, Tsu, Japan
Contribution: Data curation, Formal analysis, Investigation, Methodology, Writing - review & editing
Search for more papers by this authorRyusuke Tanaka
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Contribution: Methodology, Writing - review & editing
Search for more papers by this authorTeruo Matsushita
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Contribution: Data curation, Methodology, Writing - review & editing
Search for more papers by this authorMasaaki Miyata
Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan
Contribution: Data curation, Methodology, Supervision, Writing - review & editing
Search for more papers by this authorFunding information
This work was supported by the grant of JSPS KAKENHI (Grant Number 25850099 and 17K07951).
Abstract
We previously found a lipophilic fraction of the methanol/chloroform extract of a brown alga, Eisenia nipponica, that had an antiallergic effect in a murine ear swelling test. In this study, we purified the active component from the lipophilic fraction using high performance liquid chromatography and analyzed the mass and nuclear magnetic resonance spectra. This uncovered the phlorotannin dieckol, which exhibited antiallergic effects in an ear swelling test using mice sensitized by arachidonic acid, 12-O-tetradecanoylphorbol-13-acetate, and oxazolone. Mechanistic investigations indicated that dieckol suppressed degranulation, chemical mediator release, and the expression of mRNA such as cyclooxygenase-2, interleukin-6, and tumor necrosis factor-α in rat basophilic leukemia-2H3 cells. In summary, we isolated dieckol from E. nipponica and demonstrated its antiallergic mechanisms.
Practical applications
As the incidence of allergies increases worldwide, so too does the demand for food components with antiallergic and anti-inflammatory properties. Given this trend, we focused on a brown alga that displays a variety of bioactivities. Here, we have isolated dieckol from the antiallergic lipophilic fraction of E. nipponica and found that it possesses diverse physiological activities that may prevent lifestyle-related diseases. Consequently, dieckol or the alga containing this phlorotannin could be used as a health food ingredient to combat not only allergies, but also variety of disorders including the undesirable effects of aging.
CONFLICT OF INTEREST
The authors declare no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author, Y. Sugiura, upon reasonable request.
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