FULL ARTICLE
Saponin-rich extracts from Holothuria leucospilota mediate lifespan extension and stress resistance in Caenorhabditis elegans via daf-16
Thitinan Kitisin,
Worawit Suphamungmee,
Krai Meemon,
Thitinan Kitisin
Faculty of Science, Department of Anatomy, Mahidol University, Bangkok, Thailand
Search for more papers by this authorWorawit Suphamungmee
Faculty of Science, Department of Anatomy, Mahidol University, Bangkok, Thailand
Search for more papers by this authorCorresponding Author
Krai Meemon
Faculty of Science, Department of Anatomy, Mahidol University, Bangkok, Thailand
Correspondence
Krai Meemon, Faculty of Science, Department of Anatomy, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok 10400, Thailand.
Email: [email protected], [email protected]
Search for more papers by this authorThitinan Kitisin,
Worawit Suphamungmee,
Krai Meemon,
Thitinan Kitisin
Faculty of Science, Department of Anatomy, Mahidol University, Bangkok, Thailand
Search for more papers by this authorWorawit Suphamungmee
Faculty of Science, Department of Anatomy, Mahidol University, Bangkok, Thailand
Search for more papers by this authorCorresponding Author
Krai Meemon
Faculty of Science, Department of Anatomy, Mahidol University, Bangkok, Thailand
Correspondence
Krai Meemon, Faculty of Science, Department of Anatomy, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok 10400, Thailand.
Email: [email protected], [email protected]
Search for more papers by this authorFunding information
Mahidol University Grant; Capacity Building Program for New Researcher (2018) from the National Research Council of Thailand (NRCT); Central Instrument Facility (CIF) grant, Faculty of Science, Mahidol University
Abstract
Saponins are secondary metabolite compounds that can be found in sea cucumbers (Holothuroidea spp.). However, little is known about how saponin-rich extracts from Holothuria leucospilota can delay and prolong the lifespan of the whole organism. In this study, anti-aging effects of H. leucospilota extracts were studied on Caenorhabditis elegans. NMR analysis revealed that body wall n-butanol-extract of H. leucospilota (BW-BU) is saponin-rich. BW-BU extracts exhibited antioxidant activities by 2,2ʹ-diphenyl-2-picrylhydrazyl assay (EC50 = 10.23 ± 0.12 mg/ml) and 2,2ʹ-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid assay (EC50 = 3.91 ± 0.04 mg/ml). BW-BU extracts increased lifespan of L4 and L1 C. elegans (5.92% and 15.76%, respectively), which also increased worm growth, stress resistance, and reduced biomarkers for aging. BW-BU extracts activated DAF-16 nuclear localization and upregulated daf-16 and DAF-16 target genes expression. Taken together, this study revealed the evidences on anti-aging activities of saponin-rich extracts from H. leucospilota, which can extend lifespan of C. elegans via daf-16.
Practical applications
In recent years, age-associated chronic diseases have had a significant impact on quality of life. Many natural compounds exhibit anti-aging activities, especially in sea cucumber, H. leucospilota. Our results indicated that H. leucospilota is good for health. Extracts from H. leucospilota contain a bioactive compound that can be potentially used to promote longevity and disease prevention in aging.
CONFLICT OF INTEREST
The authors report no conflict of interest.
Supporting Information
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