Detection of oral bacterial DNA in synovial fluid
Corresponding Author
Stefan Reichert
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Address:
Stefan Reichert
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Große Steinstrasse 19, D-06108 Halle (Saale)
E-mail: [email protected]
Search for more papers by this authorMaximilian Haffner
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Search for more papers by this authorGernot Keyßer
Clinic of Internal Medicine II, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorChristoph Schäfer
Clinic of Internal Medicine II, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorJamal M. Stein
Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen, Aachen, Germany
Search for more papers by this authorHans-Guenter Schaller
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Search for more papers by this authorAndreas Wienke
Institute for Medical Epidemiology, Biostatistics and Informatics, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorHeiko Strauss
Institute for Forensic Medicine, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorSteffen Heide
Institute for Forensic Medicine, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorSusanne Schulz
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Search for more papers by this authorCorresponding Author
Stefan Reichert
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Address:
Stefan Reichert
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Große Steinstrasse 19, D-06108 Halle (Saale)
E-mail: [email protected]
Search for more papers by this authorMaximilian Haffner
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Search for more papers by this authorGernot Keyßer
Clinic of Internal Medicine II, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorChristoph Schäfer
Clinic of Internal Medicine II, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorJamal M. Stein
Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen, Aachen, Germany
Search for more papers by this authorHans-Guenter Schaller
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Search for more papers by this authorAndreas Wienke
Institute for Medical Epidemiology, Biostatistics and Informatics, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorHeiko Strauss
Institute for Forensic Medicine, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorSteffen Heide
Institute for Forensic Medicine, Martin-Luther University Halle-Wittenberg, Halle, Germany
Search for more papers by this authorSusanne Schulz
University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Search for more papers by this authorConflict of interest and source of funding statement:
The study was supported by the Martin-Luther University Halle-Wittenberg, Germany, University School of Dental Medicine, Department of Operative Dentistry and Periodontology and a grant from the “Deutsche Gesellschaft für Parodontologie (DGP)”. The authors declare that they have no conflict of interest.
Abstract
Objectives
As periodontal bacteria might be involved in the aetiology of rheumatic diseases, we analysed synovial fluid obtained from patients with rheumatoid arthritis (RA) and controls for the presence of DNA of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, and Treponema denticola.
Methods
In all, 42 patients suffering from RA (mean age 53.8 ± 16.7 years, 40.4% females) and 114 controls with no rheumatic diseases (mean age 56.1 ± 15.2 years, 52.4% females) were included. DNA from synovial fluid was isolated by QiaAmp kit (Qiagen, Hilden, Germany). Polymerase chain reactions (PCRs) specific for the 16S rRNA genes of the above specified bacteria were developed. Subgingival bacterial colonization was analysed using micro-Ident® test (HAIN-Diagnostik, Nehren, Germany).
Results
In patients with RA DNA of P. gingivalis was detected in synovial fluid more often than in controls (15.7% versus 3.5%, p = 0.045). More patients than controls harboured DNA from P. gingivalis in both, oral plaque and synovial fluid (11.9% versus. 0.9%, p = 0.030). Among the patients group the number of missing teeth was correlated with the number of joints with movement restrictions caused by RA.
Conclusions
DNA of periodontopathogens can be found in synovial fluid and oral bacteria may play a role in the pathogenesis of arthritis.
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