Original Article
Testing dispersal limits in the sea: range-wide phylogeography of the pronghorn spiny lobster Panulirus penicillatus
Matthew Iacchei,
Michelle R. Gaither,
Brian W. Bowen,
Robert J. Toonen,
Corresponding Author
Matthew Iacchei
Hawai‘i Institute of Marine Biology, Kāne‘ohe, HI, 96744 USA
Department of Oceanography, School of Ocean and Earth Science and Technology, University of Hawai‘i at Mānoa, Honolulu, HI, 96822 USA
Correspondence: Matthew Iacchei, Department of Oceanography, School of Ocean and Earth Science and Technology, University of Hawai'i at Mānoa, 1000 Pope Rd., Honolulu, HI 96822, USA.
E-mail: [email protected]
Search for more papers by this authorMichelle R. Gaither
School of Biological and Biomedical Sciences, Durham University, Durham, DH1 3LE UK
Search for more papers by this authorBrian W. Bowen
Hawai‘i Institute of Marine Biology, Kāne‘ohe, HI, 96744 USA
Search for more papers by this authorRobert J. Toonen
Hawai‘i Institute of Marine Biology, Kāne‘ohe, HI, 96744 USA
Search for more papers by this authorMatthew Iacchei,
Michelle R. Gaither,
Brian W. Bowen,
Robert J. Toonen,
Corresponding Author
Matthew Iacchei
Hawai‘i Institute of Marine Biology, Kāne‘ohe, HI, 96744 USA
Department of Oceanography, School of Ocean and Earth Science and Technology, University of Hawai‘i at Mānoa, Honolulu, HI, 96822 USA
Correspondence: Matthew Iacchei, Department of Oceanography, School of Ocean and Earth Science and Technology, University of Hawai'i at Mānoa, 1000 Pope Rd., Honolulu, HI 96822, USA.
E-mail: [email protected]
Search for more papers by this authorMichelle R. Gaither
School of Biological and Biomedical Sciences, Durham University, Durham, DH1 3LE UK
Search for more papers by this authorBrian W. Bowen
Hawai‘i Institute of Marine Biology, Kāne‘ohe, HI, 96744 USA
Search for more papers by this authorRobert J. Toonen
Hawai‘i Institute of Marine Biology, Kāne‘ohe, HI, 96744 USA
Search for more papers by this authorEditor: Gustav Paulay
Abstract
Aim
To resolve phylogeographical partitions in the pronghorn spiny lobster, Panulirus penicillatus, which has a nine-month pelagic larval phase and the broadest distribution among spiny lobsters. With samples from the Red Sea to the East Pacific, we test genetic partitions across two-thirds of the planet, in a species with one of the longest pelagic phases.
Location
Red Sea, Indian and Pacific Oceans.
Methods
A fragment of the mtDNA cytochrome c oxidase subunit I gene (COI) was resolved in 774 individuals from 28 locations, plus 55 sequences (10 locations) from public databases. Portions of COI, 12S and 16S mtDNA regions were resolved for phylogenetic analyses on a subset of individuals. Phylogenetic and population-level analyses were used to detect evolutionary partitions and dispersal barriers.
Results
Significant population structuring was detected (overall ΦST = 0.310, P < 0.001). Samples from the Red Sea and East Pacific Provinces (western and eastern range edges) comprise distinct phylogenetic lineages, divergent at d = 1.1% and 1.8%, respectively, from the Indo-Pacific. Coalescent analyses indicate a Pleistocene isolation for the Red Sea (0.71 Ma) and East Pacific (1.52 Ma). Excluding the Red Sea and East Pacific, structure across the rest of the range was comparatively low, but significant (ΦST = 0.018, P < 0.001).
Main conclusions
The Red Sea and East Pacific populations are phylogenetically distinct from populations elsewhere in the range. The East Pacific population diverged earliest, and may represent a distinct subspecies. Phylogeographical divisions align with the major Indo-Pacific biogeographical barriers, and with subsets of provincial designations from two frameworks: one based on levels of endemism and one on species composition. Tropical and temperate regions in the Pacific are significantly differentiated, potentially indicating an ecological partition. Isolation at eastern and western peripheral provinces may serve as a starting point for evolutionary diversification within this group.
Supporting Information
| Filename | Description |
|---|---|
| jbi12689-sup-0001-AppendixS1-S3.docxWord document, 3.8 MB | Appendix S1 Photographs depicting regional variation in P. penicillatus specimens. Appendix S2 Sample list, detailed methods, additional analyses. Appendix S3 Acknowledgements. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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