Seong Soo Joo, Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, Gangwon 210-702, Republic of Korea.E-mail: [email protected]Search for more papers by this author

Do Ik Lee,

Do Ik Lee

College of Pharmacy, Chung-Ang University, Seoul

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Yoon Joo Huh,

Yoon Joo Huh

College of Pharmacy, Chung-Ang University, Seoul

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Kwang Woo Hwang,

Kwang Woo Hwang

College of Pharmacy, Chung-Ang University, Seoul

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YoungWook Choi,

YoungWook Choi

College of Pharmacy, Chung-Ang University, Seoul

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Jae-Seok Choi,

Jae-Seok Choi

Institute of Environmental Research, Kangwon National University

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Sam-Yong Han,

Sam-Yong Han

Department of Chemistry, College of Natural Science

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Young-Soo Gyoung,

Young-Soo Gyoung

Department of Chemistry, College of Natural Science

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Seong Soo Joo,

Corresponding Author

Seong Soo Joo

Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, Gangwon, Republic of Korea

First published: 08 August 2011

https://doi.org/10.1111/j.2042-7158.2011.01337.x

Citations: 4

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Abstract

Objectives In the present study, we aimed to examine the anti-atopic properties of bile from the cat fish, Silurus asotus, to determine its possible use as a pharmaceutical product.

Methods The anti-atopic activities of cat fish bile were examined in a non-cell antioxidant, in-vitro assay (splenocytes and mast cells) and a 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like mouse model.

Results The results of these experiments revealed that Silurus asotus bile (SAB) scavenges radicals and protects proteins from superoxide attacks, suggesting that SAB suppresses the T helper (Th) type 2-skewed immune response. Th1/Th2 mRNA cytokines (interleukin (IL)-2, interferon (IFN)-γ and IL-4) from mouse splenocytes were effectively inhibited, and the release of β-hexosaminidase in RBL-2H3 mast cells was significantly suppressed by SAB. These results were supported by screening the Th1/Th2 cytokine mRNAs (IL-2, IFN-γ and IL-4) from lymph nodes in DNCB-treated mice. More dramatic results were observed in the histological changes at higher SAB concentrations (5%) compared to the therapeutic control, visualized using hematoxylin–eosin (H&E) staining.

Conclusions The results presented in this study suggest that SAB may provide functional advantages with regard to treating atopic dermatitis because of its antioxidant and immune-suppressive effects.

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Volume63, Issue10

October 2011

Pages 1327-1335

Alternative therapeutic advantages of catfish bile on atopic dermatitis: protection of T cell-mediated skin disease via antioxidant activities

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Alternative therapeutic advantages of catfish bile on atopic dermatitis: protection of T cell-mediated skin disease via antioxidant activities

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