Age Macular Degeneration-Lipidomic Study: Relevance and interest of Lipidomic study in screening, follow-up and etiopathogeny of AMD
Abstract
Purpose
To evaluate the lipidomic study impact on AMD diagnosis, screening, etiopathogenie and interrelations,correlations between those 2 entities.
Methods
AMD: 30 AMD patients. 3 Groups: A:10 first stage AMD patients,B:10 Atrophy AMD patients, C:10 Neovascular AMD patients. Ophthalmologic exam:ETDRS visual acuity (VA), complete ophthalmic examination,Fundus examination, autofluorescence imaging (FAF), optical coherence tomography (Spectral Domain OCT) and fluorescein angiography (FA) and ICG when Neovascular complication.Lipidomic Study: Blood tests and analysis, all lipids qualitative, quantitative analysis, all the same for all patients, whatever group. Blood test is done during ophthalmologic exam. Plasma congelation “snap frost” in liquid nitrogen after total blood centrifugation, then liquid-liquid extraction for lipids analysis:neutral lipid by GC, as well as fatty acid but after BF3 methanol derivation,phospholipids by LC-MS directly,as sphingolipids but firstly hydrolysed. Polyinsatured fatty acids metabolites preparation: protein precipitation then pre-concentration by SPE(solid phase extraction) before m'analyses by LC-MS.
Results
Analysis will determine qualitative,quantitative lipids values in each patients group, each of them proportion, characterization, singularity; so, characterization, prevalence, specifics of and for each group. Lipidomic study’ evaluation, identification, classification in AMD patients groups allow AMD screening,follow-up, particularly according to AMD type and stage. Lipidomic study have biomarker feature, let AMD prevention, etiopathogenic concept.
Conclusions
Lipidomics study and so better AMD characterization allow better diagnosis, follow-up, screening of AMD. Interrelations and correlations between AMD and Lipidomics lead to better etiopathogenie understanding and therapeutics prospects.
