Migration of lens epithelial cells and IOL drug soaking
Summary
Purpose
To assess the effect of EGFR Inhibitor Erlotinib and the downstream Inhibitor Erufosin (PI3K) soaked into intraocular lenses (IOLs) on human lensepithelialcell (LEC) proliferation in vitro.
Methods
Foldable IOLs were incubated with Erufosin or Erlotinib. Intraocular lenses of the same lot served as uncoated controls. Each IOL was placed into cell cultre containing proliferating human LECs. Cell survival was tested by the XTT-dye reduction assay 5 days later. Furthermore IOLs were put into the Gotoh anterior chamber model. In addition, soaked IOLs were implanted into the human capsular bags and braught to cell-culture. The time until full cell-coverage of the capsular bag was measured.
Results
Erufosin (P<0.05) and Erlotinib (P<0.05) coated IOLs attenuated human LEC proliferation in all above described models. For both substances soaked hydrophilic acrylic IOLs were more effective inhibitors of human LEC proliferation than coated hydrophobic acrylic.
Conclusions
Results show that both substances are suitable agents for IOL-soaking without linker molecules. Soaked IOLs can inhibit human LEC proliferation in our models and might become of clinical relevance in future.
