FLUORESCENCE AND CIRCULAR DICHROISM STUDIES ON THE PHYCOERYTHROCYANINS FROM THE CYANOBACTERIUM
Westiellopsis prolifica
P. S. Maruthi Sai
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
Botanisches Institut der Universität, Menzinger Straβe 67, 8000 München 19, Germany
*Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel.
Search for more papers by this authorS. Siebzehnrübl
Botanisches Institut der Universität, Menzinger Straβe 67, 8000 München 19, Germany
Search for more papers by this authorS. Mahajan
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
Search for more papers by this authorCorresponding Author
H. SCHEER
Botanisches Institut der Universität, Menzinger Straβe 67, 8000 München 19, Germany
†To whom correspondence should be addressed.Search for more papers by this authorP. S. Maruthi Sai
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
Botanisches Institut der Universität, Menzinger Straβe 67, 8000 München 19, Germany
*Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel.
Search for more papers by this authorS. Siebzehnrübl
Botanisches Institut der Universität, Menzinger Straβe 67, 8000 München 19, Germany
Search for more papers by this authorS. Mahajan
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
Search for more papers by this authorCorresponding Author
H. SCHEER
Botanisches Institut der Universität, Menzinger Straβe 67, 8000 München 19, Germany
†To whom correspondence should be addressed.Search for more papers by this authorAbstract
Two phycoerythrocyanin (PEC) fractions have been obtained from the phycobilisomes of the cyanobac-terium Westiellopsis prolifica ARM 365. They have been characterized by absorption, fluorescence and circular dichroism spectroscopy. One of them is spectroscopically similar to a PEC trimer known from other organisms. Whereas efficient energy transfer from its violin (α-84) to the cyanin (β-84, 155) chromophores is efficient in the trimer (αβ it is impeded after dissociation to the monomer (α,β). A second fraction of PEC which we earlier termed PEC(X) (Maruthi Sai et al., Photochem. Photobiol.55,119–124, 1992), exhibited the spectral properties similar to that of the α-subunit of PEC from Mastigocladus laminosus. With this highly photoactive fraction, the circular dichroism spectra of the violobilin chromophore in both photoreversible states were obtained.
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