Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191
Haoyue Wang
Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China
These authors contributed equally to this work
Search for more papers by this authorRuilin Sun
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Graduate School of Chinese Academy of Sciences, Beijing 100049, China
These authors contributed equally to this work
Search for more papers by this authorGuoxiang Liu
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Graduate School of Chinese Academy of Sciences, Beijing 100049, China
Search for more papers by this authorMinghui Yao
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Graduate School of Chinese Academy of Sciences, Beijing 100049, China
Search for more papers by this authorCorresponding Author
Jian Fei
School of Life Science and Technology, Tongji University, Shanghai 200092, China
*Corresponding authors: Jian Fei: Tel, 86-21-65982429; Fax, 86-21-65982429; E-mail, [email protected] Hebai Shen: Tel, 86-21-64321800; Fax, 86-21-54242640; E-mail, [email protected]Search for more papers by this authorCorresponding Author
Hebai Shen
Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China
*Corresponding authors: Jian Fei: Tel, 86-21-65982429; Fax, 86-21-65982429; E-mail, [email protected] Hebai Shen: Tel, 86-21-64321800; Fax, 86-21-54242640; E-mail, [email protected]Search for more papers by this authorHaoyue Wang
Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China
These authors contributed equally to this work
Search for more papers by this authorRuilin Sun
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Graduate School of Chinese Academy of Sciences, Beijing 100049, China
These authors contributed equally to this work
Search for more papers by this authorGuoxiang Liu
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Graduate School of Chinese Academy of Sciences, Beijing 100049, China
Search for more papers by this authorMinghui Yao
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Graduate School of Chinese Academy of Sciences, Beijing 100049, China
Search for more papers by this authorCorresponding Author
Jian Fei
School of Life Science and Technology, Tongji University, Shanghai 200092, China
*Corresponding authors: Jian Fei: Tel, 86-21-65982429; Fax, 86-21-65982429; E-mail, [email protected] Hebai Shen: Tel, 86-21-64321800; Fax, 86-21-54242640; E-mail, [email protected]Search for more papers by this authorCorresponding Author
Hebai Shen
Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China
*Corresponding authors: Jian Fei: Tel, 86-21-65982429; Fax, 86-21-65982429; E-mail, [email protected] Hebai Shen: Tel, 86-21-64321800; Fax, 86-21-54242640; E-mail, [email protected]Search for more papers by this authorThis work was supported by grants from the National Basic Research Program of China (No. 2002CB713803), the National Natural Science Foundation of China (No. 20573075) and the Science and Technology Commission of Shanghai Municipality (Nos. 06XD14014, 0752nm028, 07DZ22303, 06DZ05137 and 54319939)
Abstract
Studies on the DNA-binding properties of transcription factors are important in searching for the downstream genes regulated by these factors. In the present study, we report on the DNA-binding property of a Cy s2His2-type transcription factor, zinc finger protein 191 (Zfpl91), which has been newly found to play a significant role in mice. By constructing a fusion protein containingthe DNA-binding domain of Zfpl91, we characterized target DNA by determining the protein's binding specificity and dependence on zinc. The data showed that the DNA-binding domain of Zfpl91 can specifically bind to the TCAT repeat motif and that there is a cooperative effect amongthe target DNA's multiple binding sites. Furthermore, the binding reaction is dependent on zinc. This work provides a foundation for further studies on the role of Zfpl91 in gene regulation and development.
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