Expression of Cbl-interacting protein of 85 kDa in MPTP mouse model of Parkinson's disease and 1-methyl-4-phenyl-pyridinium ion-treated dopaminergic SH-SY5Y cells
Minjuan Bian
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
These authors contributed equally to this work
Search for more papers by this authorMei Yu
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
These authors contributed equally to this work
Search for more papers by this authorShanzheng Yang
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorHui Gao
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorYalin Huang
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorChunguang Deng
Shanghai Nan Fang Model Organism Research Center, Shanghai 201203, China
Search for more papers by this authorYanqin Gao
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorFengyan Sun
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorCorresponding Author
Fang Huang
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
*Corresponding author: Tel, 86-21-54237296; Fax, 86-21-64174579; E-mail: [email protected]Search for more papers by this authorMinjuan Bian
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
These authors contributed equally to this work
Search for more papers by this authorMei Yu
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
These authors contributed equally to this work
Search for more papers by this authorShanzheng Yang
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorHui Gao
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorYalin Huang
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorChunguang Deng
Shanghai Nan Fang Model Organism Research Center, Shanghai 201203, China
Search for more papers by this authorYanqin Gao
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorFengyan Sun
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
Search for more papers by this authorCorresponding Author
Fang Huang
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
*Corresponding author: Tel, 86-21-54237296; Fax, 86-21-64174579; E-mail: [email protected]Search for more papers by this authorThis work was supported by the grants from the National Natural Science Foundation (30570633) and the Shanghai Metropolitan Fund for Research and Development (04BZ14005 and 07DJ14005)
Abstract
The newly discovered Cbl-interacting protein of 85 kDa (CIN85) is involved in many cellular processes, but its functions in the brain and in neurodegenerative diseases remain unclear. In this paper, we investigated the distribution of CIN85 protein in different regions of adult mouse brain using Western blot analysis and immunohistochemistry, and found that CIN85 was ubiquitously expressed in mouse brain. In the striatum and substantia nigra, two regions most deeply affected in Parkinson's disease, the level of CIN85 protein was relatively high. In the MPTP mouse model of Parkinson's disease, the expression of CIN85 in the striatum and substantia nigra was complicated. But in 1-methyl-4-phenyl-pyridinium ion-treated human dopaminergic SH-SY5Y cells, the expression of CIN85 increased dramatically. Knocking down of CIN85 by short hairpin RNA reduced SH-SY5Y cell death. Therefore, CIN85 might play different roles in the dopaminergic cell line and in the nigrostriatum of mouse brain under neurotoxin challenge.
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