Role of ornithine decarboxylase in breast cancer
Wensheng Deng
Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
These authors contributed equally to this work
Search for more papers by this authorXian Jiang
The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical Medical School, Harbin Medical University, Harbin 150001, China
These authors contributed equally to this work
Search for more papers by this authorYu Mei
Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
Search for more papers by this authorJingzhong Sun
Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
Search for more papers by this authorRong Ma
Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
Search for more papers by this authorXianxi Liu
Experimental Center of Medical Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China
Search for more papers by this authorHui Sun
Experimental Center of Medical Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China
Search for more papers by this authorCorresponding Author
Hui Tian
Department of Thoracic Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
*Corresponding authors:Xueying Sun: Tel/Fax, 86-451-53643628; E-mail, [email protected]Hui Tian: Fax, 86-531-86927544; E-mail, [email protected]Search for more papers by this authorCorresponding Author
Xueying Sun
The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical Medical School, Harbin Medical University, Harbin 150001, China
Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland 1005, New Zealand
*Corresponding authors:Xueying Sun: Tel/Fax, 86-451-53643628; E-mail, [email protected]Hui Tian: Fax, 86-531-86927544; E-mail, [email protected]Search for more papers by this authorWensheng Deng
Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
These authors contributed equally to this work
Search for more papers by this authorXian Jiang
The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical Medical School, Harbin Medical University, Harbin 150001, China
These authors contributed equally to this work
Search for more papers by this authorYu Mei
Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
Search for more papers by this authorJingzhong Sun
Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
Search for more papers by this authorRong Ma
Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
Search for more papers by this authorXianxi Liu
Experimental Center of Medical Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China
Search for more papers by this authorHui Sun
Experimental Center of Medical Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China
Search for more papers by this authorCorresponding Author
Hui Tian
Department of Thoracic Surgery, Qilu Hospital of Shandong University, Jinan 250012, China
*Corresponding authors:Xueying Sun: Tel/Fax, 86-451-53643628; E-mail, [email protected]Hui Tian: Fax, 86-531-86927544; E-mail, [email protected]Search for more papers by this authorCorresponding Author
Xueying Sun
The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical Medical School, Harbin Medical University, Harbin 150001, China
Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland 1005, New Zealand
*Corresponding authors:Xueying Sun: Tel/Fax, 86-451-53643628; E-mail, [email protected]Hui Tian: Fax, 86-531-86927544; E-mail, [email protected]Search for more papers by this authorThis work was supported by a grant from the National Natural Science Foundation of China (30571844 and 30571808)
Abstract
Ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis that decarboxylates ornithine to putrescine, has become a promising target for cancer research. The aim of this study is to investigate the role of ODC in breast cancer. We detected expression of ODC in breast cancer tissues and four breast cancer cell lines, and transfected breast cancer cells with an adenoviral vector carrying antisense ODC (rAd-ODC/Ex3as) and examined their growth and migration. ODC was overexpressed in breast cancer tissues and cell lines compared with non-tumor tissues and normal breast epithelial cells, and there was a positive correlation between the level of ODCmRNA and the staging of tumors. The expression of ODC correlated with cyclin D1, a cell cycle protein, in synchronized breast cancer MDA-MB-231 cells. Gene transfection of rAd-ODC/Ex3as markedly down-regulated expression of ODC and cyclin D1, resulting in suppression of proliferation and cell cycle arrest at G0–G1 phase, and the inhibition of colony formation, an anchorage-independent growth pattern, and the migratory ability of MDA-MB-231 cells. rAd-ODC/Ex3as also markedly reduced the concentration of putrescine, but not spermidine or spermine, in MDA-MB-231 cells. The results suggested that the ODC gene might act as a prognostic factor for breast cancer and it could be a promising therapeutic target.
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